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Laser microdissection and nucleic acid purification - a Leica - QIAGEN ,,, cooperation

Using this optimized protocol, transcripts from formalin-fixed tissues were detected one cycle earlier than when using the protocol for cryosections (Figure 3B). Low-abundance transcripts, such as TNF-alpha, can be detected and quantified from lasermicrodissected samples, provided that the samples are properly handled. Using the optimized protocols for each type of lasermicrodissected tissue, TNF- mRNA was easily detected in cryosections but was not detected after 40 cycles with RNA isolated from formalin-fixed tissues (Figure 3C).

Conclusions
  • These results emphasize the importance of sample preparation and nucleic acid isolation and analysis from lasermicrodissected samples. Flash-frozen,
    cryosectioned tissues provide a better source for nucleic acid isolation than formalin-fixed tissues.

  • QIAGEN protocols for RNA and DNA isolation have been developed in conjunction with the Leica AS LMD System.



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