Navigation Links
Laser microdissection and nucleic acid purification - a Leica - QIAGEN ,,, cooperation

Using this optimized protocol, transcripts from formalin-fixed tissues were detected one cycle earlier than when using the protocol for cryosections (Figure 3B). Low-abundance transcripts, such as TNF-alpha, can be detected and quantified from lasermicrodissected samples, provided that the samples are properly handled. Using the optimized protocols for each type of lasermicrodissected tissue, TNF- mRNA was easily detected in cryosections but was not detected after 40 cycles with RNA isolated from formalin-fixed tissues (Figure 3C).

Conclusions
  • These results emphasize the importance of sample preparation and nucleic acid isolation and analysis from lasermicrodissected samples. Flash-frozen,
    cryosectioned tissues provide a better source for nucleic acid isolation than formalin-fixed tissues.

  • QIAGEN protocols for RNA and DNA isolation have been developed in conjunction with the Leica AS LMD System.



'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
2. Working With Laser Capture Microdissection Samples
3. Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions
4. Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
5. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
6. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
7. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
8. Walkaway automation for nucleic acid purification
9. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
10. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
11. Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins
Post Your Comments:
(Date:4/28/2015)... Spartan Bioscience today announced that Paul ... by the Ottawa Chamber of Commerce as one of ... selected from over 240 applicants based on his business ... Spartan, Dr. Lem founded Plexagen Diagnostics, and also served ... a Medical Degree from the University of Ottawa, and ...
(Date:4/27/2015)... , April 27, 2015  For about a ... is their ability to utilize and catabolize glucose ... cancer cells is called the ,Warburg phenomenon", that ... of high oxygen presence, leading to enhanced lactate ... cells still choose glucose fermentation. A variety of ...
(Date:4/27/2015)... Diego, Calif. (PRWEB) April 27, 2015 ... of drug and chemical toxicity carries implications for advancing ... speaker April 28 at National University , the ... and presentation by Dr. Hanzlik, a professor at the ... drug metabolism and drug design, starts at 5 p.m. ...
(Date:4/27/2015)... BRUNSWICK, New Jersey , 27 ... société de découverte médicamenteuse spécialisée dans ... antibiotiques destinés à soigner les infections ... aujourd,hui la présentation de données prouvant ... majeur, le TXA709, dans la lutte ...
Breaking Biology Technology:Spartan Bioscience Announces Paul Lem Named Ottawa’s Top 40 Under 40 2Study Indicates Cancer Cells Alter Glucose Metabolism to Enhance Energy Production and Cell Growth 2Dr. Robert Hanzlik Discusses Pharmaceutical Testing Models at Event Hosted by National University 2Dr. Robert Hanzlik Discusses Pharmaceutical Testing Models at Event Hosted by National University 3TAXIS Pharmaceuticals démontre la puissance clinique du TXA709 dans la lutte contre la résistance aux antibiotiques 2TAXIS Pharmaceuticals démontre la puissance clinique du TXA709 dans la lutte contre la résistance aux antibiotiques 3TAXIS Pharmaceuticals démontre la puissance clinique du TXA709 dans la lutte contre la résistance aux antibiotiques 4TAXIS Pharmaceuticals démontre la puissance clinique du TXA709 dans la lutte contre la résistance aux antibiotiques 5TAXIS Pharmaceuticals démontre la puissance clinique du TXA709 dans la lutte contre la résistance aux antibiotiques 6
... in a line of reports paints a bleak picture ... such financing could be at its lowest level in ... to year's end. , ,The Money Tree Survey ... and the National Venture Capital Association, indicates that, unless ...
... Chippewa Valley representatives hope to score points with medical ... the largest medical device tradeshow in the Midwest. ... Minneapolis Convention Center on November 2nd and 3rd is ... company executives. , ,The twelve representatives include economic development ...
... Madison, Wis. - Sonic Foundry Inc. announced today ... "E-rate" program, which offers schools around the country the ... , ,The E-rate program is designed to provide discounts ... for the cost of education technology. Funded technology includes ...
Cached Biology Technology:While some bemoan lack of venture capital, entrepreneur touts `bootstrapping' for cash 2While some bemoan lack of venture capital, entrepreneur touts `bootstrapping' for cash 3While some bemoan lack of venture capital, entrepreneur touts `bootstrapping' for cash 4Chippewa Valley reps hope to score at Twin Cities tradeshow 2
(Date:4/2/2015)... 2015 In the current ... outperforms the strongest competitors, showing outstanding performance in ... system is capable of a throughput of 5.000.000 ... to be ten times faster than most competitors ...      (Photo: http://photos.prnewswire.com/prnh/20150402/738673-a ) ...
(Date:4/1/2015)... BOSTON , April 1, 2015   Medisafe ... 1.5 million users across iOS and ... has begun integrating biofeedback into the platform to allow ... health outcomes. For the first time, patients will be ... missing a dose impacts important biometrics, such as glucose ...
(Date:3/24/2015)... and Markets ( http://www.researchandmarkets.com/research/hdgz7h/global_iris ) has announced ... 2015-2019" report to their offering. The ... at a CAGR of 23.5 percent over the period ... and the growth prospects of the Global Iris Recognition ... size, the report considers the revenue generated from sales ...
Breaking Biology News(10 mins):DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 2DERMALOG AFIS Emerges as Performance Winner in NIST Benchmark Test 3Medisafe Announces Launch of Biometric Integration, Enabling Patients to Stay Adherent to Their Meds and See Tangible Health Results 2Global Iris Recognition Market 2015-2019 with 3M Cogent, Bioenable Technologies, Cross Match Technology, Iris ID Systems & Safran Dominating 2
... determined that milk thistle extract, a widely used ... on the course of patients with alcoholic liver ... These findings are published in The American ... studies, milk thistle extracts have been shown to ...
... more than half of children with sore throat, exceeding ... antibiotics for 27 percent of children who received an ... 9 issue of JAMA. , Pharyngitis (inflammation of the ... to family medicine physicians and pediatricians, according to background ...
... young scientists in the University of York's Department of ... patients some of the unpleasant and dangerous side-effects of ... together with post-doctoral research fellow Mark Godber, have developed ... tumours more efficiently. , Now, the group has secured ...
Cached Biology News:Children overprescribed antibiotics for sore throat 2Young scientists turn to gel to ease side-effects of cancer treatment 2
TUNEL Apoptosis Detection Kit...
... assay kit measures NOS activity by monitoring ... This assay is simple, sensitive, and specific ... with both crude and purified enzyme preparations. ... for 50 total reactions. Radiolabeled arginine and ...
HiTrap NHS-activated HP, 1 x 5 ml. Category: Chromatography Systems & Accessories, Systems....
Mouse Axl MAb (Clone 175128)...
Biology Products: