Navigation Links
Laser microdissection and nucleic acid purification - a Leica - QIAGEN ,,, cooperation

DNA from LMD Samples Using Optimized QIAGEN Protocols Figure 2 Cells were laser-microdissected from flash-frozen and formalin-fixed human colon tissue using the Leica AS LMD System. DNA was isolated following the optimized QIAGEN protocol, either with (+) or without () cleanup using the QIAquick PCR Purification Kit. PCR of the GAPDH gene was carried out for 35 cycles using the HotStarTaq Master Mix Kit and primers from QIAGEN Operon. M: markers. TaqMan Analysis of RNA from LMD Samples Figure 3 Cells were laser-microdissected from flash-frozen and formalin-fixed human colon tissue using the Leica AS LMD System. RNA was isolated following the optimized QIAGEN protocols (Protocol 1: optimized protocol for frozen tissue; Protocol 2: optimized protocol for formalin-fixed tissue). Real-time, quantitative RT-PCR was carried out on an ABI PRISM 7700 Sequence Detector using the QuantiTect Probe RT-PCR Kit and primers and probes for -actin or TNF-alpha, as indicated. All reactions were run in quadruplicate. A Sample amplification plot for -actin using RNA purified from flash-frozen tissue. B Threshold cycles for -actin. C Threshold cycles for TNF-alpha. Protocol 2, optimized for formalin-fixed tissues, includes digestion with QIAGEN Proteinase K for higher RNA yield and quality.
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
2. Working With Laser Capture Microdissection Samples
3. Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions
4. Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
5. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
6. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
7. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
8. Walkaway automation for nucleic acid purification
9. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
10. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
11. Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins
Post Your Comments:
(Date:1/15/2014)... Calif. (PRWEB) January 15, 2014 Cynvenio, ... and management through the genomic analysis of tumor cells ... Therapy Finder™, a web-based cancer decision support application powered ... option with Cynvenio’s ClearID™ genomic test, Therapy Finder will ...
(Date:1/15/2014)... This webinar will focus on EMA ... assessment in biosimilars. , Regulatory frameworks are evolving many ... however the complex nature of biopharmaceuticals makes the demonstration ... extremely challenging. Based on the specific aspects of biosimilar ...
(Date:1/15/2014)... Reston, VA (PRWEB) January 15, 2014 ... education have continued their sponsorship of an annual competition ... interest in, and access to, innovative STEM study. The ... , TEAMS: Tests of Engineering Aptitude, Mathematics, and ...
(Date:1/15/2014)... January 15, 2014 2013 was a ... Brain State Technologies®. They saw continued independent research led ... who were awarded a $1 million grant from the ... “Brain and Behavior” a peer reviewed journal, Amy Grant ...
Breaking Biology Technology:Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 4Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 5Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3
... there was a monopoly phone service prior to the 1984 ... exclusivity in the territory and we will provide universal service ... a geographic subscriber base, the traditional phone companies ensured that ... fair price. There was one price across the board. That ...
... - Does the Wisconsin Alumni Research Foundation's ... for approval? , ,The foundation's stem cell patent is ... it is overly broad and is suppressing scientific research. ... the more than 300 academic licenses it has issued ...
... to clarify the definition of records for the treatment ... incorporate them into Electronic Health Records, has been signed ... 650 creates an exception to current law pertaining to ... to mental health records when they are treating patients ...
Cached Biology Technology:Does redefining universal service help bridge the digital divide? 2Does redefining universal service help bridge the digital divide? 3Does redefining universal service help bridge the digital divide? 4Does redefining universal service help bridge the digital divide? 5WARF stem cell patent faces long and winding road 2WARF stem cell patent faces long and winding road 3WARF stem cell patent faces long and winding road 4WARF stem cell patent faces long and winding road 5
(Date:7/9/2014)... by researchers at the NIH,s National Center ... collaborators to treat sickle cell disease has ... The drug candidate, Aes-103, is the first ... mechanism of sickle cell disease. Baxter now ... for regulatory approval and commercialization. , Sickle ...
(Date:7/9/2014)... protect neuronal function and increase the survival rate ... her team, School of Pharmaceutical Sciences, Southwest University, ... mice were administered with low dose of levothyroxine ... aged rats exhibited an obvious improvement in cognitive ... to 93%. The underlying mechanism was demonstrated that ...
(Date:7/9/2014)... less fit in terms of cardiorespiratory capacity, strength ... southern adolescents are more obese and present higher ... from the centre-north of Europe. , These are ... study conducted by scientists from the University of ... 25 other European research groups. The study compared ...
Breaking Biology News(10 mins):First drug candidate from NIH program acquired by biopharmaceutical company 2First drug candidate from NIH program acquired by biopharmaceutical company 3First drug candidate from NIH program acquired by biopharmaceutical company 4Adolescents from southern Europe are less fit and more obese than central-northern European peers 2
... delivers new insight about rapid toxin evolution in venomous snakes: ... race with opossums, a group of snake-eating American marsupials. Although ... this fact has not been factored into previous explanations for ... usually seen as a feeding, or trophic, adaptation. But new ...
... contrast agents that sneak into bacteria disguised as glucose ... sensitivity and specificity. These agents -- called maltodextrin-based imaging ... other inflammatory conditions. "These contrast agents fill the ... of bacteria in vivo and distinguish infections from other ...
... 18, 2011) Researchers from the John Theurer Cancer ... 14 cancer-related studies during the recently concluded American Society ... June 3 7, 2011 in Chicago. The studies ... treatment outcomes, and patient quality of life issues. ...
Cached Biology News:Rapid venom evolution in pit vipers may be defensive 2Rapid venom evolution in pit vipers may be defensive 3New contrast agents detect bacterial infections with high sensitivity and specificity 2New contrast agents detect bacterial infections with high sensitivity and specificity 3John Theurer Cancer Center researchers shared 14 leading edge studies at recent ASCO meeting 2John Theurer Cancer Center researchers shared 14 leading edge studies at recent ASCO meeting 3John Theurer Cancer Center researchers shared 14 leading edge studies at recent ASCO meeting 4John Theurer Cancer Center researchers shared 14 leading edge studies at recent ASCO meeting 5John Theurer Cancer Center researchers shared 14 leading edge studies at recent ASCO meeting 6
... enables early selection of the best ... quantitative data for sensitivity, dynamic range, ... Furthermore quantitative antibody fingerprints can be ... your antibodies., The full-service package includes ...
... faster than western blotting. Get results sooner and ... on gel detection. Eliminates the need for a ... All reagents are ready-to-use. No reagents to ... Only 2 reagents required. Guarantees a simple to ...
... and filter paper sandwiches are composed of ... sheets of thick filter paper that are ... paper sandwich. These 7 x 8.5 cm ... fit Ready Gel-sized gels and are supplied ...
The VersArray hybridization chamber is used for manual (coverslip) hybridization of microarrays. The chamber accommodates 2 slides and is watertight to allow incubation of slides in a water bath. Dim...
Biology Products: