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Laser microdissection and nucleic acid purification - a Leica - QIAGEN ,,, cooperation

Stefanie Schrer, Werner Wittke,* and Christian Kupfer
QIAGEN GmbH, Hilden, Germany

* Leica Microsystems, Wetzlar, Germany Laser microdissection (LMD) provides a powerful technology to isolate specific cells or cell groups for further analysis. This eliminates the problems of cell-type heterogeneity within tissue samples by providing homogeneous cell populations for analysis. The technique allows highly specific comparisons, for example, of cancer cells or other diseased cells with normal cells in the same tissue sample.

Now QIAGEN has joined forces with Leica Microsystems, a leading supplier of highprecision optical microscopes, to bring you complete systems for LMD with nucleic acid purification and analysis of the dissected samples.

Laser Microdissection Using the Leica
AS LMD System
A major benefit of laser microdissection using the Leica AS LMD System is excision of cells from sections with the highest precision and without physical contact. Following cutting by a laser, the excised part falls into a collection tube under gravity, ensuring contaminationfree processing and minimizing sample damage. The Leica AS LMD System enables excision of cells or metaphase chromosomes from a wide range of samples, including paraffin-embedded sections, frozen sections,blood smears, and stained and immunolabeled specimens. The Leica Laser Microdissection Microscope provides an easy-to-use, automated method for microdissection (Figure 1).
QIAGEN has developed a set of protocols for is
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