Navigation Links
Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

, Cat. # 12-550-19)
◊ Acetylthiocholine Iodide (Sigma, Cat. # A-5751)
◊ Sodium Acetate (Sigma, Cat. # S-2889)
◊ Sodium Citrate (Fluka, Cat. # 71402)
◊ Cupric Sulfate (Sigma, Cat. # C-1297)
◊ CapSure HS LCM Caps (Arcturus, Cat. # LCM0214)
◊ FastStart Taq DNA Poymerase (Roche, Cat. # 2 032 926)
◊ dNTP Mix PCR Grade (Invitrogen, Cat. # 18427-013)
◊ UltraPureTM Agarose (Invitrogen, Cat. # 15510-027)
◊ UltraPureTM 10x TAE (Invitrogen, Cat. # 15558-026)
◊ Ethidium Bromide (Sigma, Cat. # E-1510)
◊ Tissue-Tek OCT Compound (Sakura, Cat. # 4538)
◊ DEPC Treated Water (Ambion, Cat. # 9920)


Equipment and Labware

◊ General equipment necessary:
◊ Disposable gloves
◊ Flow hood
◊ Dry ice
◊ Liquid nitrogen
◊ Ice or cold block
◊ Nuclease-free, aerosol resistant tips
◊ RNase-free microcentrifuge tubes
◊ Scale
◊ KimwipesTM or similar lint-free towels

The following laboratory equipment is required to microdissect muscle fiber properly:
◊ Cryostat
◊ Cryostat knife
◊ Arcturus PixCell II Laser Capture Microdissection System with fluorescence package
◊ QImaingTM Retiga 1300 cooled mono 12-bit digital camera

The following laboratory equipment is required for RNA isolation and analysis by RT-PCR:
◊ Incubation oven
◊ Microcentrifuge
◊ Arcturus alignment tray
◊ Thermo Cycler with heated lid

'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
2. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
3. Working With Laser Capture Microdissection Samples
4. Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions
5. Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
6. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
7. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
8. Primary cells, rat heart muscle
9. High-throughput gene expression analysis to screen for anti-asthma drugs
10. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
11. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
Post Your Comments:
(Date:9/2/2015)... , Sept. 2, 2015 ... announced the addition of Jain PharmaBiotech,s new ... to their offering. ... pharmaceutical and biotechnology industries is anticipated. Nanotechnology ... drug development - from formulations for optimal ...
(Date:9/2/2015)... Sep. 02, 2015 ... the addition of Jain PharmaBiotech,s new report ... their offering. This report follows the ... that can be objectively measured and evaluated as ... as well as pharmacological responses to a therapeutic ...
(Date:9/2/2015)... , Sept. 2, 2015  Neurotech Pharmaceuticals, ... enrolled in the multicenter Phase 2 clinical trial ... long-term treatment of recurrent subfoveal choroidal neovascularization secondary ... is a unique vascular endothelial growth factor (VEGF) ... implant. "This landmark proof-of-concept study will ...
(Date:9/2/2015)... Israel , September 2, 2015 ... announced the intent of the National Institute of Allergy and ... Health (NIH) within the Department of Health and Human Services ... held in the United States in ... BiondVax,s universal flu vaccine candidate, Multimeric-001 (M-001), as a primer ...
Breaking Biology Technology:Global Nanobiotechnology Applications, Markets and Companies Report 2015-2024 2Global Nanobiotechnology Applications, Markets and Companies Report 2015-2024 3Updated 2015 Biomarkers Report - Latest Technologies, Markets and Companies Insights to 2024 with over 1,000 References 2Updated 2015 Biomarkers Report - Latest Technologies, Markets and Companies Insights to 2024 with over 1,000 References 3Neurotech Announces First Patient Enrolled in Novel Anti-VEGF Encapsulated Cell Therapy Study 2Neurotech Announces First Patient Enrolled in Novel Anti-VEGF Encapsulated Cell Therapy Study 3Neurotech Announces First Patient Enrolled in Novel Anti-VEGF Encapsulated Cell Therapy Study 4BiondVax Announces Intent to Launch a Phase 2 Trial in the United States in Collaboration with the U.S. National Institutes of Health 2BiondVax Announces Intent to Launch a Phase 2 Trial in the United States in Collaboration with the U.S. National Institutes of Health 3
... Wis. - With plans to erect as many as ... Systems is thinking about needs well beyond its existing ... ,Steve Dickmann, Epic's chief administrative officer, said the company anticipates ... existing employee count of 2,500. In addition, there remains plenty ...
... the director of technology programs for the University of Wisconsin-Madison ... forefront of trends in information technology workforce development. In part ... for courses that help IT professionals in their progression from ... week. , , Scott Converse also is a featured ...
... Dan Hanlon sees a lot of parallels between riding a motorcycle ... He has skinned his knees attempting to do both. , ,Hanlon ... speak during the Wisconsin Alumni Research Foundation's Gilson ... in managing an emerging-growth company. , ,While he figures to be ...
Cached Biology Technology:Epic Systems will build well beyond current staff size 2Motorcycling entrepreneur to speak in Madison on success, failure, capital 2Motorcycling entrepreneur to speak in Madison on success, failure, capital 3
(Date:8/12/2015)... Minn. , Aug. 12, 2015   MedNet ... supports the entire spectrum of clinical research, is proud ... , the company,s comprehensive SaaS-based eClinical technology platform, has ... during the first two quarters of 2015.   Q2 ... second largest quarters measured by contract value sold in ...
(Date:8/12/2015)... , Aug. 12, 2015  Synaptics Inc. (NASDAQ: ... interface solutions, today announced that four of its ... solution, have officially been named FIDO Certified™ by ... of the certification, Synaptics, Natural ID™ fingerprint solutions ... Authentication Framework (UAF) standard and are interoperable among ...
(Date:8/10/2015)... -- The latest 364 page report from business ... global border security market . Visiongain assesses ... Now: Border security is hard to achieve and ... critical issue you need to know about - and more ... of how this will impact your company and the border ...
Breaking Biology News(10 mins):MedNet Solutions Experiences Explosive Corporate Growth 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 3Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 2Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 3Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 4Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 5Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 6Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 7Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 8Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 9
... University research group has discovered that aggressive melanoma ... to proper embryo formation. , An article ... advanced online issue of the journal Nature Medicine. The ... growth factor by injecting aggressive melanoma cells into ...
... An ancient avenue for producing cellular energy, the glycolytic ... therapies. , A team of Harvard Medical School ... LDHA, in a variety of fast-growing breast cancer cells, ... mice. Control animals carrying tumor cells with an intact ...
... that may have broader implications for understanding kidney ... Philadelphia have identified a second gene that gives ... that affects multiple organs. The Children's Hospital team ... disease. , The researchers found that mutations in ...
Cached Biology News:Malignant melanoma cells secrete protein required for embryo formation 2Attacking cancer's sweet tooth is effective strategy against tumors 2Attacking cancer's sweet tooth is effective strategy against tumors 3Gene discovery may shed light on kidney disease 2
pH-insensitive fluorescence (pH 2-12)...
HOXC11 Antibody...
PPOX Immunogen: PPOX (AAH02357, 378 a.a. ~ 478 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Rabbit polyclonal to Raf1 ( Abpromise for all tested applications). entrezGeneID: 5894 SwissProtID: P04049...
Biology Products: