Navigation Links
Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

Sven Fraterman and Neal Rubinstein, Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia


Abstract
To locate gene products in distinct muscle fiber populations of different muscle allotypes, a novel protocol was established. A rapid method to distinguish muscle fiber types using histochemistry and immunohistochemistry was used to provide criteria for their selective isolation by laser capture microdissection while preserving messenger RNA (mRNA). Polymerase chain reaction showed a differential expression pattern of muscle specific genes in different muscle fibers in laser captured material.


Introduction
While vertebrate limb muscles have traditionally been the paradigm for studies of skeletal muscle myogenesis, a few atypical muscle groups such as extraocular muscles (EOMs) have distinct functional specializations and patterns of gene expression1. Differences between muscle groups (called allotypes) have been the subject of research for the past decades. The limb and EOM allotypes differ in their pattern of innervation. All limb fibers and 80% of EOM fibers are singly innervated fibers (SIFs) which have only one neuromuscular junction. About 20% of EOM fibers are multiply-innervated fibers (MIFs): they have multiple neuromuscular junctions2. In the past, immunohistochemistry and in situ hybridization were mainly used to study differences between muscle fiber populations3,4, While useful, these techniques are limited in their capability to assess multiple gene products from a mixed muscle fiber population. Moreover, the mixed fiber populations of these muscles make it impossible to study the composition of individual fiber types. To overcome these problems, laser capture microdissection was used to isolate different muscle fiber populations and analyze multiple gene products by polymerase chain reaction
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
2. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
3. Working With Laser Capture Microdissection Samples
4. Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions
5. Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
6. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
7. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
8. Primary cells, rat heart muscle
9. High-throughput gene expression analysis to screen for anti-asthma drugs
10. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
11. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
Post Your Comments:
(Date:4/24/2015)... Diego, CA (PRWEB) April 24, 2015 ... Diego and other San Diego area universities, including UCSD ... 28 to judges from prominent local, national and nonprofit ... funding in the 5th Annual Social Innovation Challenge ... have proposed unique social venture ideas addressing a diverse ...
(Date:4/24/2015)... /PRNewswire/ - BioAmber Inc. (NYSE: BIOA ) has ... of bio-succinic acid used to produce a new ... that Bayer MaterialScience has begun commercializing.    ... eco, a series of bio-based waterborne polyurethane dispersions ... 65%.  IMPRANIL ® eco has been developed ...
(Date:4/24/2015)... 2015 PAREXEL International Corporation (NASDAQ: ... announced that Partnerships in Clinical Trials named PAREXEL,s ... Clinical Innovator of the Year.  The honor recognizes Mr. ... Company,s Perceptive MyTrials ® Data-Driven Monitoring solution. ... the 2015 Partnership Awards in Boston ...
(Date:4/23/2015)... 2015 The Thomas Brain Health ... a groundbreaking pilot study to see if early ... as little as three to six months. , ... nutritional medicine—will aggressively target and simultaneously treat the ... aging brain. These include oxidative stress, neuroinflammation, mitochondrial ...
Breaking Biology Technology:5th Annual Social Innovation Challenge at University of San Diego - Students Pitch to Live Judging Panel to Take Home $75K 25th Annual Social Innovation Challenge at University of San Diego - Students Pitch to Live Judging Panel to Take Home $75K 3Bayer MaterialScience Names BioAmber As Its Supplier For New Product Line - Bio-succinic acid forms backbone of innovative new bio-based polyurethanes for textiles 2Bayer MaterialScience Names BioAmber As Its Supplier For New Product Line - Bio-succinic acid forms backbone of innovative new bio-based polyurethanes for textiles 3Bayer MaterialScience Names BioAmber As Its Supplier For New Product Line - Bio-succinic acid forms backbone of innovative new bio-based polyurethanes for textiles 4PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 2PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 3PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 4PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 5New Study Looks to Reverse Alzheimer’s Disease 2New Study Looks to Reverse Alzheimer’s Disease 3
... developed a new test to detect the levels of vitamin ... simpler diagnosis that could help to avoid the potentially fatal ... today, 23 June 2011, in IOP Publishing,s Journal of ... breath test to more accurately measure vitamin B12. ...
... 2011 Clinical Research Management, (ClinicalRM) a full-service ... applied research, clinical trials, and regulatory support, announced ... $97MM research support contract titled Support for Military ... performed at the Walter Reed Army Institute of ...
... 22, 2011 CMC Biologics today announced that it ... steel biopharmaceutical production facility at its Bothell campus and ... new logo and a compelling tagline. These recent changes ... (Logo:   http://photos.prnewswire.com/prnh/20110502/SF93356LOGO ) The ...
Cached Biology Technology:A breath of fresh air for detecting vitamin B12 deficiency 2ClinicalRM (formerly CRM) Awarded $97MM U.S. Army Contract to Support Military Clinical Research for the Warfighter 2CMC Biologics Expands Its Production Facility and Rebrands Its Corporate Identity 2
(Date:4/2/2015)... 2015 At its 2015 ACMG Annual Clinical ... , the American College of Medical Genetics and Genomics ... its Board.  Members of the ACMG Board of Directors ... and advancing its policies and programs. ACMG is the ... "It,s an eventful time in medical genetics and genomics.  ...
(Date:3/31/2015)... , March 31, 2015  Elephant Talk Communications ... a global provider of Software Defined Network Architecture (ET ... announced total revenue of approximately $20.4 million for the ... restated $19.5 million for the year ended December 31, ... 2014, the Company had completed its multi-year transition away ...
(Date:3/24/2015)... , Mar. 24, 2015 Research and Markets ( ... Voice Biometrics - Global Strategic Business Report" report to their ... and Voice Biometrics in US$ Thousands by the following Segments: Face ... the US, Canada , Japan ... Middle East & Africa ...
Breaking Biology News(10 mins):American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 4Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 5Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 6
... 2007 Men who have lower-pitched voices have more ... found. And their study suggests that for reproductive-minded ... The study, published in Biology Letters, offers insight into ... how we choose our mates. In previous studies, ...
... that could improve a class of drugs based on antibodies, ... structural changes in an antibody that will improve its effectiveness. ... create a new version of cetuximab, a drug commonly used ... 10 times greater affinity than the original molecule. The ...
... Legacy of Regulatory Submissions Leadership Strengthened with Clinical ... Trials Services and Standards-Based Data Management, ... Solutions,Inc. (ISI) announced today it agreed to terms ... Sciences industry moving to,standards-based regulatory submissions, the acquisition ...
Cached Biology News:MIT model could improve some drugs' effectiveness 2MIT model could improve some drugs' effectiveness 3Image Solutions, Inc. Acquires Zurich Biostatistics, Inc. 2Image Solutions, Inc. Acquires Zurich Biostatistics, Inc. 3
... on Macintosh operating systems to predict ... sequence up to 3,200 bases, which ... and GC clamps for mutation detection ... constant denaturing gel electrophoresis (CDGE), and ...
Request Info...
... cell GT cell and PowerPac basic power supply ... nucleic acids in agarose gels. The Mini-Sub cell ... cables and electrodes, a 7 x 7 cm ... gates, and two 1.5 mm fixed-height combs (8- ...
... Solution Polymorphic DNA Technologies offers a ... complete solution for SNP discovery by performing ... primers, PCR amplification, DNA sequencing and data ... SNP discovery, Polymorphic uses the gold standard ...
Biology Products: