Navigation Links
Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

serially-diluted standards and samples were analyzed by gel electrophoresis to confirm the presence of the specific amplicon (Fig. 3B). To show that LCM enriches macrophage-specific transcripts, RNA was extracted either from whole sections (analogous to homogenized tissue) or from LCM-derived CD68 immuno-positive macrophage foam cells. Real-time quantitative RT-PCR for CD68 was performed on equivalent amounts of RNA (100 pg) and the results normalized to the control gene, cyclophilin A. As shown in Fig. 3C, the LCM-derived RNA was significantly enriched in the mRNA for the macrophage specific marker, CD68, compared to whole section RNA (33.6-fold). To determine the potential cellular contamination of the laser captured cells by adjacent medial smooth muscle cells (SMC) either from non-specific tissue adherence to the thermoplastic film or from imprecise laser beam positioning, smooth muscle cellspecific α-actin was measured by real-time quantitative RT-PCR. As shown in Fig. 3D, in contrast to the level of α-actin in RNA from whole sections, in the LCM-derived RNA, α-actin expression was at background levels after 40 cycles of amplification. These results show that lesional macrophage foam cells can be precisely and selectively isolated from atherosclerotic vessels by CD68-guided LCM. To test whether RNA derived by LCM of macrophage foam cells can be utilized to quantitatively assess the transcriptional regulation of target genes implicated in atherosclerosis, laser captured lesional macrophage RNA from proximal aortas of LPS and control stimulated apolipoprotein E-/- mice were analyzed. Mice were administered either an intraperitoneal injection of the bacterial endotoxin lipopolysaccharide (LPS) (100 g) or vehicle only and, ~4 hours after treatment, the proximal aortas were processed for each mouse as described above. The relative gene transcript levels of the following genes were measured: vascular cell adhesion molecule-1 (VCAM-1), intercellular adh
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11

Related biology technology :

1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
2. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
3. Working With Laser Capture Microdissection Samples
4. Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
5. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
6. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
7. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
8. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
9. Epitope-Tagging Vectors for Functional Analysis in Yeast
10. Map and Link Human Genetic Disorders with SSLP Analysis
11. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
Post Your Comments:
(Date:9/29/2014)... Claremont, CA (PRWEB) September 30, 2014 ... 1 million burn injuries require medical attention. In ... and brings serious complications to battlefield medical care. ... in Iraq and Afghanistan. Burn wounds heal slowly, ... that can be excessive, physically debilitating and functionally ...
(Date:9/29/2014)... in Iraq and Afghanistan, more than 300,000 soldiers have ... caused by exposure to bomb blasts and in ... of traumatic brain injury can range from the mild, ... impairments in memory and cognition. , Since 2007, ... importance and complexity of this problem, and has made ...
(Date:9/29/2014)... Research and Markets  has announced the ... & Services, End-User , Application - Global Forecast to ... The global oligonucleotide synthesis market is expected to reach ... growing at a CAGR of 9.8% from 2014 to ... the basis of products and services, applications, end users, ...
(Date:9/29/2014)... challenges in food security, emerging diseases and biodiversity ... published online in Science Express that ... of Arizona College of Agriculture and Life Sciences. ... of nine scientists has reviewed progress in addressing ... and environmental management using approaches that consider evolutionary ...
Breaking Biology Technology:Faster Healing with Fewer Scars 2Modeling shockwaves through the brain 2Modeling shockwaves through the brain 3Modeling shockwaves through the brain 4Oligonucleotide Synthesis (Diagnostics, PCR, QPCR, Gene Synthesis, NGS, DNA, RNAi) Market - Global Forecast to 2019 2Oligonucleotide Synthesis (Diagnostics, PCR, QPCR, Gene Synthesis, NGS, DNA, RNAi) Market - Global Forecast to 2019 3Evolutionary biology: It's not just for textbooks anymore 2Evolutionary biology: It's not just for textbooks anymore 3
... Cell Therapeutics, Inc. (CTI) (Nasdaq and MTA: CTIC) management will present at the 2009 BIO International Convention at ... of Building A, Level 3. , ... ... 10:40 AM Eastern/4:40 PM Central European/7:40 AM Pacific, ...
... (OTC Bulletin Board: TBIO) today announced financial results for the ... are presented in the tables that follow.The Company reported a ... first quarter of 2009 as compared to a net income ... of 2008.Net sales from continuing operations were $5.0 million during ...
... Microtest Laboratories , a leading single source provider ... current suite of analytical testing and ... dissolution testing is the perfect complement to ... Scientific Founder of Microtest Laboratories ( www.microtestlabs.com ). ...
Cached Biology Technology:Transgenomic, Inc. Reports First Quarter 2009 Results 2Transgenomic, Inc. Reports First Quarter 2009 Results 3Transgenomic, Inc. Reports First Quarter 2009 Results 4Transgenomic, Inc. Reports First Quarter 2009 Results 5Transgenomic, Inc. Reports First Quarter 2009 Results 6Transgenomic, Inc. Reports First Quarter 2009 Results 7Microtest Labs Adds Dissolution Testing to its Suite of Analytical and Stability Testing Services 2Microtest Labs Adds Dissolution Testing to its Suite of Analytical and Stability Testing Services 3
(Date:9/29/2014)... President Juan Manuel Santos has presented Dr. Cristin ... Society, with the prestigious Order of San Carlos. ... citizens, foreign civilians and military officers who have ... the field of international relations. , An international ... Samper was invited to the Colombian Residence in ...
(Date:9/29/2014)... article published online in Behavioral Brain Research ... brain as a result of chronic alcohol exposure that ... , Clinical assessments and research indicate that individuals with ... This can occur when people are actively drinking, when ... abstaining. , "Sleep-wake disturbances can last for months, ...
(Date:9/29/2014)... or esoteric. Yet in the modern world, it plays ... and a decisive role in the discovery and development ... UC Santa Barbara,s Paul Atzberger, a professor in the ... engineering, often works in areas where science and math ... Proceedings of the National Academy of Science ...
Breaking Biology News(10 mins):At the interface of math and science 2At the interface of math and science 3At the interface of math and science 4
... Materials engineers have created a new type of membrane ... be used for environmental cleanup, water purification and industrial ... conventional filters for separating oil from water and works ... usually mutually exclusive. Researchers attached the material to a ...
... the Netherlands Royal Academy of Arts and Sciences (KNAW) ... a new research programme: the Joint Scientific Thematic Research ... and the Academy will work together on developing bilateral ... and the Academy launched the joint research programme during ...
... Journal of Preventive Medicine special issue on climate ... Climate Changing People" conference today in Los Angeles. ... MD, DrPH, speaking about the impacts of climate change ... public health challenge. Health professionals need to understand the ...
Cached Biology News:New filtering technology has environmental, industrial applications 2New filtering technology has environmental, industrial applications 3NWO and Academy launch research program with China 2Issues at intersection of climate change and health impact global well-being 2Issues at intersection of climate change and health impact global well-being 3Issues at intersection of climate change and health impact global well-being 4
Tumor Suppressor Sampler Kit 10 mu g each...
AMAP Multi Site-directed Mutagenesis Kit Research Focus: other Storage Temperature: -20C Shipping Temperature: dry ice...
Umbilical cord blood eosinophils, For immunohistochemistry (IHC)...
Slide Chambers are convenient for performing pre-hybridization incubations and post-hybridization washes with tissue sections mounted on standard glass microscope slides. Each chamber holds up to 5 s...
Biology Products: