Navigation Links
Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

reagent (prepared at least 30 minutes in advance according to manufacturers instructions) for 1 min. at RT (see Special Considerations, Note 1).
  • Rinse slides in 100mM Tris-HCl Buffer pH. 7.5 for 1 min. at RT.
  • Apply Vector Red chromogenic substrate until adequate intensity of staining develops (~5 - 10 min). Immerse slides in RNase-Free water to stop reaction.
  • Sections are counterstained with Harris modified hematoxylin for ~ 5 sec.
  • Sections are subsequently dehydrated in graded ethanols (95% 2X, 1 min each, 100% 3X, 3 min each ) and cleared in xylene (3X, 2 min each). Allow slides to air dry under a vacuum hood for 30 min (see Special Considerations, Note 2).

  • C. LCM and RNA Extraction

    Laser capture was performed under direct microscopic visualization on the CD68positively stained areas by activating a thermoplastic film mounted on optically transparent LCM caps over selected regions. 30 proximal aortic sections from each apolipoprotein E-deficient mouse were microdissected in regions immunostained positive for CD68/Macrosialin and with morphologically-identifiable cells having the characteristic foamy appearance. Approximately 300 laser pulses were performed on each section using the PixCell II LCM Instrument. The following parameters were used: 15m laser diameter, 40 mW power, 3.0 msec duration.



    1. RNA was extracted/isolated using the PicoPure RNA Isolation Kit as per manufacturers instructions.
    2. Examine the surface of the LCM cap under the microscope to ensure complete cell extraction.
    3. To eliminate potential genomic DNA contamination, RNA samples were treated with the DNase (Qiagen) directly onto the column as per manufacturers instructions.
    4. RNA concentra
      '"/>


    Source:


    Page: All 1 2 3 4 5 6 7 8 9 10 11

    Related biology technology :

    1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
    2. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
    3. Working With Laser Capture Microdissection Samples
    4. Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
    5. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
    6. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
    7. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
    8. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
    9. Epitope-Tagging Vectors for Functional Analysis in Yeast
    10. Map and Link Human Genetic Disorders with SSLP Analysis
    11. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
    Post Your Comments:
    (Date:7/10/2014)... 10, 2014 Women in the ... to an additional family planning option. Sayana® Press ... at all levels of the health system and ... a widely used contraceptive—Pfizer’s Depo-Provera® (depot medroxyprogesterone acetate)—with ... is the first of four African countries expected ...
    (Date:7/10/2014)... 2014 Product and process impurities ... abundance and are often “lost in the noise,” ... Join presenters Dr. Rowel Tobias, Senior Scientist, Protein ... Head of Quality at Nanotherapeutics, Inc., to lear ... speed detection and quantitation while achieving the required ...
    (Date:7/10/2014)... 2014 Deep Knowledge Ventures last ... on ‘Commercialising Longevity Research’ and welcomed a host ... London Bioscience Innovation Center for the event, organised ... Analytics Ltd. The meeting highlighted the need for ... for age-related disease, as well as the crucial ...
    (Date:7/10/2014)... Cleveland, Ohio (PRWEB) July 10, 2014 ... in ion channel-expressing cell lines, and the gap that ... new cells that expand its industry-leading portfolio of validated ... focuses on ion channel-expressing cell lines, as ion channels ... able to control this activity creates the potential to ...
    Breaking Biology Technology:Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 2Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 3Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 4Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 5Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 6Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks 2DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 2DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 4DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 5DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 6Leader in Ion Channel Research Announces Newly Validated and Re-Engineered Cells 2Leader in Ion Channel Research Announces Newly Validated and Re-Engineered Cells 3
    ... 13 Nile Therapeutics, Inc.,(Nasdaq: NLTX ), today ... present at the Rodman & Renshaw 5th Annual Global ... at Le Meridian Beach,Plaza Hotel in Monte Carlo, Monaco. ... the status of Nile,s current clinical development,programs., The ...
    ... Foamix Offers New Intravaginal Foam Technology with Improved Usability & ... ... Israel, May 13 Despite the high incidence,of women,s health conditions ... and ovules, a recent survey,found that an alarming majority of women ...
    ... with CelVida Yields First Milestone, NEW YORK, May ... pioneering the pre-disease collection, processing and long-term,storage of adult ... a,site has been secured for a new stem cell ... Robin Smith, MD MBA, NeoStem,s Chairman and Chief ...
    Cached Biology Technology:Nile Therapeutics to Present at the Rodman & Renshaw 5th Annual Global Healthcare Conference 272% of Women Are Dissatisfied With Their Current Treatment of Vaginal Infection, Survey Finds 2NeoStem to Expand Stem Cell Collection Center Network to Florida 2NeoStem to Expand Stem Cell Collection Center Network to Florida 3
    (Date:7/10/2014)... Fe, Albuquerque, and other major cities in New Mexico, ... treated municipal wastewater rather than precious potable water supplies. ... 40% of all golf courses receive treated effluent. Reusing ... , Additionally, golf courses and homeowners alike fertilize their ... fertilizer is nitrate. A New Mexico State University turfgrass ...
    (Date:7/10/2014)... , June 27, 2014  The American ... National Institute of Standards and Technology (NIST) and ... its academic research and forensic science expert members ... Board is an element of the NIST,s Organization ... are among the first appointments made to the ...
    (Date:7/10/2014)... NEW YORK , June 27, 2014 /PRNewswire/ ... against Provectus Biopharmaceuticals, Inc. ("Provectus" or the "Company")(NYSE-MKT: ... action, filed in United States District Court, Middle ... Division, is on behalf of a class consisting ... otherwise acquired Provectus securities between December 17, 2013 ...
    Breaking Biology News(10 mins):'Tailored' water -- the latest in lawn care 2Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 2Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 3SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 3
    ... for a University of Alberta paleontology researcher, who after months of ... as a new species of pterosaur, a flying reptile that lived ... stumped when the small piece of jaw bone was first pulled ... A,s paleontology department. "It could have been from a dinosaur, ...
    ... 4, 2011) − Scientists at the University of Kentucky have ... break up blood clots, speeds up the progress of prion ... plasminogen a promising new target for the development of drugs ... senior author Chongsuk Ryou, a researcher at the UK Sanders-Brown ...
    ... Lithuania, Jan. 10, 2011 Neurotechnology , ... announced the release of MegaMatcher 4.0 , ... fingerprint, iris, facial and palmprint biometrics in a ... MegaMatcher 4.0, Neurotechnology has incorporated palmprint technology along ...
    Cached Biology News:Research identifies drug target for prion diseases, 'mad cow' 2New MegaMatcher 4.0 from Neurotechnology Integrates Fingerprint, Iris, Facial and Palmprint Biometrics In One High-Performance SDK 2New MegaMatcher 4.0 from Neurotechnology Integrates Fingerprint, Iris, Facial and Palmprint Biometrics In One High-Performance SDK 3
    AVOID FREEZE/THAW CYCLES. Recognizes the ~32 kDa DNase IIα chain and the ~40 kDa proenzyme. DNase II causes both DNA condensation and DNA fragmentation. ...
    Vented Caps for 75 cm2 Flasks...
    I-Ab MHC Class II...
    ... System, Series 4000 incorporates the critical design ... biologists in biomarker research. The Series 4000 ... for converting biomarker discoveries to biomarker assays ... 4000 is designed to fit into the ...
    Biology Products: