Navigation Links
Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

ta en bloc. Transect the heart at the lower poles of the atria and perpendicular to them. This plane is parallel to that of the aortic valve.
  • Place the upper half on the heart (containing the atria) in an embedding mold filled with OCT cryoembedding medium, and allow it to infiltrate into the cavity of the heart.
  • Position the heart flat with the cut surface on the bottom of the embedding mold. Fill the embedding mold with OCT and freeze on dry ice.
  • Store specimens at 80 C.
  • Section tissue blocks at 6 m thickness and mount on positively charged Color Frost Plus slides.

  • B. Immunodetection of CD68+ Macrophages for LCM.

    Standard immunohistochemical staining protocols usually require prolonged incubation periods in aqueous media, which results in significant degradation of RNA. To overcome this limitation, a modified rapid immunostaining protocol was developed for macrophage-specific CD68/Macrosialin that does not significantly affect RNA yields (<12% reduction of total RNA in immunostained versus non-immunostained whole tissue sections) (11).

    1. Allow frozen sections to air dry for 1 min and then fix in 70% ethanol for 15 sec followed by cold acetone for 5 min.
    2. Incubate slides with anti-mouse CD68/macrosialin IgG (1:10 dilution) in 4% goat blocking serum (made in PBS) supplemented with SuperaseIn RNase inhibitor (0.4U/l) for 1 min at room temperature (RT).
    3. Rinse slides in PBS 3 times for 5 min each.
    4. Incubate slides with biotinylated rabbit-anti-rat IgG mouse-adsorbed secondary antibody (20g/ml final) in 4% goat blocking serum supplemented with SuperaseIn RNase inhibitor (0.4U/l) for 1 min at RT.
    5. Rinse slides in PBS 3 times for 5 min each.
    6. Incubate slides with Vectastain ABC Alk-Phos
      '"/>


    Source:


    Page: All 1 2 3 4 5 6 7 8 9 10 11

    Related biology technology :

    1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
    2. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
    3. Working With Laser Capture Microdissection Samples
    4. Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
    5. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
    6. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
    7. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
    8. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
    9. Epitope-Tagging Vectors for Functional Analysis in Yeast
    10. Map and Link Human Genetic Disorders with SSLP Analysis
    11. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
    Post Your Comments:
    (Date:5/21/2015)... N.J. (PRWEB) May 21, 2015 ... circulation audit and been admitted to BPA Worldwide ... BPA Worldwide will track audience data for Specialty ... coverage. , “By becoming a member of ... dedication to providing our clients with the most ...
    (Date:5/21/2015)... (PRWEB) May 21, 2015 uBiome, ... announced a partnership with PicnicHealth, a healthcare company ... Participants diagnosed with Inflammatory Bowel Disease (IBD) will ... a complementary uBiome research kit. Both companies were ... , For more information on this ...
    (Date:5/21/2015)... W. R. Grace & Co. ... Worms, Germany has received good manufacturing practice (GMP) ... International Pharmaceutical Excipient Council (IPEC) Foundation. ... SYLOID® FP brand of pharmaceutical grade excipient silica ... Curtis Bay, Maryland (USA) and Sorocaba, Brazil locations. ...
    (Date:5/21/2015)... SURPRISE, ARIZ. (PRWEB) May 21, 2015 ... E. Webb Medical Center (BDWMC), Sun Health Foundation funded ... medical center’s new inpatient pharmacy with the most up-to-date ... to open June 2. The pharmacy's 45 staff members ... much larger 4,895-square-foot space in the coming days. ...
    Breaking Biology Technology:Specialty Pharmacy Times Joins BPA Worldwide 2uBiome Partners with PicnicHealth 2uBiome Partners with PicnicHealth 3Grace European Facility Receives GMP Excipient Certification for SYLOID® FP Silica Gel 2Sun Health Foundation Provides $3.25M to Expand, Relocate Banner Del E. Webb Medical Center Pharmacy 2Sun Health Foundation Provides $3.25M to Expand, Relocate Banner Del E. Webb Medical Center Pharmacy 3
    ... Global Science, Scale and Talent Required to Meet Growing Demand ... ... will enable farmers to produce enough grain to meet the growing ... enterprises, regulators and policymakers from around the globe take a more ...
    ... Fundamental Mechanism of Angiogenesis is More Complicated Than ... Angiogenesis Website THOUSAND OAKS, Calif., April 16 ... that results from several preclinical studies investigating potential ... 2009 American Association for Cancer Research (AACR) Annual ...
    ... Financial ResultsVANCOUVER, April 16 /PRNewswire-FirstCall/ - Angiotech Pharmaceuticals, Inc. (NASDAQ: ... call discussing its financial results for the first quarter ended ... AM ET (8:00 AM Pacific)., Dial-in information ... follows:, North America (toll-free): ...
    Cached Biology Technology:DuPont Leader: Agriculture is Up to Global Productivity Challenge 2DuPont Leader: Agriculture is Up to Global Productivity Challenge 3DuPont Leader: Agriculture is Up to Global Productivity Challenge 4Preclinical Data From Amgen's Oncology Pipeline to be Presented at AACR 2Preclinical Data From Amgen's Oncology Pipeline to be Presented at AACR 3Preclinical Data From Amgen's Oncology Pipeline to be Presented at AACR 4Preclinical Data From Amgen's Oncology Pipeline to be Presented at AACR 5Preclinical Data From Amgen's Oncology Pipeline to be Presented at AACR 6Preclinical Data From Amgen's Oncology Pipeline to be Presented at AACR 7
    (Date:5/19/2015)... Sweden , May 18, 2015 ... fingerprint sensors FPC1020, FPC1025, FPC1145, FPC1155 and FPC1035 from one ... from June until and including Q3 2015 and the sensors ... . Communicated order values for 2015 hereby amount to ... for Q1 2015 of 140 MSEK and a number of ...
    (Date:5/18/2015)... 18, 2015 Fingerprint Cards (FPC) has ... FPC1145, FPC1155 and FPC1035 from one if its module partners. ... including Q3 2015 and the sensors will be used by ... values for 2015 hereby amount to 740 MSEK to date. ... 140 MSEK and a number of smaller orders not separately ...
    (Date:5/11/2015)... 11, 2015  Synaptics Incorporated (NASDAQ: SYNA ... today announced the appointment of Wajid Ali ... Officer, reporting to Rick Bergman , President ... Financial Officer, Kathleen Bayless , who announced ... Mr. Ali brings extensive financial management expertise to ...
    Breaking Biology News(10 mins):Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Synaptics Appoints Wajid Ali as Senior Vice President and Chief Financial Officer 2
    ... method for fighting skin wrinkles has been developed at ... Quality Sciences. In her doctoral research at the ... antioxidant that delays the aging process by countering the ... conducted her research under the supervision of Zecharia ...
    ... of the most basic features of a living organism. ... in turn control others genes whose activity turns on ... cycle, energy production, DNA repair, cellular suicide etc). Many ... special proteins (transcription factors) to 6 - 10 nucleotide ...
    ... Smoking tobacco is no longer considered sexy, but it may prove ... the online open access journal BMC Genomics could help explain why ... who have never smoked. A Canadian team led by ... took samples from the lungs of 24 current and former smokers, ...
    Cached Biology News:Antioxidant to retard wrinkles discovered by Hebrew University researcher 2Gene regulation in humans is closer than expected to simple organisms 2Smoking turns on genes -- permanently 2
    ... refrigerated cold traps offers a choice of ... solvents or high volatility solvents. Ideal to ... evaporators. Also used for maintaining vapor-free gas ... in electron microscopes. Can be used as ...
    96 Well Plate Sterile, RE bottom; consistent medium binding affinity for hydrophobic complexes and proteins....
    ... Incubated Console Orbital Shakers combine high ... unequalled programming power. The patented ... the unit to run with heavy ... filtration system reduces contamination. The ...
    The Holten mini vertical flow workbench provides a particle-free environment for sample protection against airborne contaminants....
    Biology Products: