Navigation Links
Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

#12223-012)

The following laboratory equipment is required to complete the protocol:
◊ Disposable gloves
◊ Dry ice
◊ Cryostat
◊ ABI Prism 7700 Sequence Detection System (Applied Biosystems)
◊ RNase AWAY (Fisher Scientific, Cat. #14-375-35)
◊ Nuclease-free pipette tips
◊ MicroAmp optical tubes (Applied Biosystems, Cat. #N801-0933)
◊ MicroAmp optical caps (Applied Biosystems, Cat. #N801-0935)
◊ Nusieve 3:1 agarose (BioWhittaker Molecular Applications)
◊ Immunostaining jars


RNase-free Technique
All reagents were maintained under RNase-free sterile conditions.

  1. Wear disposable gloves and change them frequently.
  2. Pipettmen are wiped with RNase AWAY prior to use according to manufacturers instructions.
  3. Cryosections are temporarily stored in the cryostat and later into a -80 C freezer to prevent RNA degradation.
  4. Use RNase-free solutions (water prepared by treatment with
  5. Concentration of antibodies and duration of incubations have been optimized so as to preserve the quality of RNA and to yield specific staining having a high signal/noise ratio.
  6. Addition of SuperaseIn RNase Inhibitor to the antibody cocktails during immunostaining is recommended as it prevents RNA degradation during the incubation steps. (NOTE: Do not add the SuperaseIn to the ABC Alk-Phos enzymatic reaction as it will completely inhibit the staining).


Special Considerations

  1. We have observed that addition of the SuperaseIn RNase inhibitor to the alka- line phophatase en
    '"/>


Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11

Related biology technology :

1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
2. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
3. Working With Laser Capture Microdissection Samples
4. Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
5. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
6. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
7. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
8. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
9. Epitope-Tagging Vectors for Functional Analysis in Yeast
10. Map and Link Human Genetic Disorders with SSLP Analysis
11. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
Post Your Comments:
(Date:1/22/2015)... (PRWEB) January 22, 2015 Selexis SA, ... mammalian Research Cell Banks (RCBs) used for drug discovery ... Cell Banks will include Next-Generation Sequencing (NGS) ... architecture de-risks biologic manufacturing by ensuring the integrity of ...
(Date:1/22/2015)... , Jan. 22, 2015   Cypher Genomics, Inc., ... Inc. (NASDAQ: SQNM ), the leading ... for next generation noninvasive prenatal tests (NIPT). Through ... technology, called Mantis™, to advance analysis of clinically-relevant ...
(Date:1/22/2015)... N.J. , Jan. 22, 2015  Derma Sciences, ... regeneration company focused on advanced wound care, announces ... placental tissues, have been added to the Premier, ... increases the opportunity for the AMNIOEXCEL® and AMNIOMATRIX® ...
(Date:12/24/2014)... , Dec. 24, 2014 PlasmaTech Biopharmaceuticals, Inc. (NASDAQ: PTBI, ... announced the closing of an underwritten public offering of 3,500,000 ... an aggregate 3,500,000 shares of common stock, at an offering ... warrants have a per share exercise price of $5.00, are ...
Breaking Biology Technology:Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2Cypher Genomics and Sequenom Announce Development Agreement 2Cypher Genomics and Sequenom Announce Development Agreement 3Cypher Genomics and Sequenom Announce Development Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 3Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 5PlasmaTech Biopharmaceuticals, Inc. Announces Closing of Public Offering 2PlasmaTech Biopharmaceuticals, Inc. Announces Closing of Public Offering 3
... BioTime, Inc. (OTCBB:BTIM) announced today that Robert N. ... of Directors. Dr. Butler is the founder, Chief ... Center-USA, a non-profit international research, policy, and education ... live longer and better, and advise society on ...
... service revenue grows approximately 33% ... - Operating margin of 9.9% ... ... 6 PAREXEL International,Corporation (Nasdaq: PRXL ) today announced its financial results for the,fourth quarter and ...
... BioSciences announced,today the successful acquisition of funding ... the development of a long lasting, safe, ... a consortium of,microbicide researchers from four major ... from the portfolio of microbicides,being developed by ...
Cached Biology Technology: Dr. Robert N. Butler Appointed to Board of Directors of BioTime, Inc. 2 Dr. Robert N. Butler Appointed to Board of Directors of BioTime, Inc. 3 Dr. Robert N. Butler Appointed to Board of Directors of BioTime, Inc. 4PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 2PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 3PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 4PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 5PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 6PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 7PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 8PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 9PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 10PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 11PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 12PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 13PAREXEL Reports Fourth Quarter and Fiscal Year 2008 Financial Results 14ImQuest Receives $6.4 M Five Year Award from NIH to Develop Long-Lasting Combination Topical Microbicides To Prevent HIV Transmission 2ImQuest Receives $6.4 M Five Year Award from NIH to Develop Long-Lasting Combination Topical Microbicides To Prevent HIV Transmission 3
(Date:12/15/2014)... , Dec. 12, 2014 Research and Markets ... the "Global Facial Recognition Market 2015-2019" report ... Facial recognition is a technology used ... the overall facial feature of a person such as ...
(Date:12/11/2014)... Dec. 09, 2014 Research and Markets ... http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has announced the addition of the ... offering. One major trend emerging in ... Multimodal biometric systems utilize more than one characteristic of ...
(Date:12/11/2014)... PAUL, Minn. , Dec. 10, 2014  Data ... physiologic monitoring, has released a new series of ... of preclinical toxicology researchers. M series, part of ... toxicologists collect the best possible physiologic data when ...
Breaking Biology News(10 mins):Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2New telemetry implants expected to change how large animal toxicology studies are conducted 2
... in Spanish . Xabier Basogain Olabe ... Engineering in Bilbao, is leading this project, known as Bonis. ... visual monitoring of epidemiology in isolated areas of Paraguay and ... practical trials will begin shortly and it is hoped to ...
... Two genes have been associated with autistic spectrum ... Researchers writing in BioMed Central,s newly launched journal ... for two brain proteins, LRRN3 and LRRTM3, were significantly ... the Wellcome Trust Centre for Human Genetics, University of ...
... twist. No small feat. Now the flexible and stretchable ... heart with better resolution and speed than that of ... the University of Illinois at Urbana-Champaign and the University ... silicon electronics device used for a medical application. The ...
Cached Biology News:University of the Basque Country develops system for identifying illnesses in Paraguay 2University of the Basque Country develops system for identifying illnesses in Paraguay 3Hugging the heart electronically 2Hugging the heart electronically 3
...
...
Mouse polyclonal antibody to TNPO2 - transportin 2 (importin 3, karyopherin beta 2b)...
Mouse monoclonal antibody to CDC2L5 - cell division cycle 2-like 5 (cholinesterase-related cell division controller)...
Biology Products: