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Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

esion molecule1 (ICAM-1), and monocyte chemoattractant1(MCP-1) (see Table I for gene primer sequences). As shown in Table II, LPS stimulation increased the expression of the Nuclear Factor-κB-mediated inflammatory genes, thereby confirming the ability of LCM and quantitative real-time RT-PCR to measure the regulation of genes after a perturbation or in pathologic (vs. normal) condition.




References
1. Smith, J.D., Trogan, E., Ginsberg, M., Grigaux, C., Tian, J., and Miyata, M. 1995. Decreased atherosclerosis in mice deficient in both macrophage colony- stimulating factor (op) and apolipoprotein E. Proc Natl Acad Sci USA. 92:8264-8268.

2. Qiao, J.H., Tripathi, J., Mishra, N.K., Cai, Y., Tripathi, S., Wang, X.P., Imes, S., Fishbein, M.C., Clinton, S.K., Libby, P., Lusis, A.J., et al. 1997. Role of macrophage colony-stimulating factor in atherosclerosis: studies of osteopetrotic mice. Am J Pathol. 150:1687-1699.

3. de Villiers, W.J., Smith, J.D., Miyata, M., Dansky, H.M., Darley, E., and Gordon, S. 1998. Macrophage phenotype in mice deficient in both macrophage-colony- stimulating factor (op) and apolipoprotein E. Arterioscler Thromb Vasc Biol. 18:631-640.

4. Chong, P.H., and Bachenheimer, B.S. 2000. Current, new and future treatments in dyslipidaemia and atherosclerosis. Drugs. 60:55-93.

5. Brewer, H.B., Jr. 2000. The lipid-laden foam cell: an elusive target for therapeutic intervention. J Clin Invest. 105:703-705.

6. Plutzky, J. 1999. Atherosclerotic plaque rupture: emerging insights and opportunities. Am J Cardiol. 84:15J20J.

7. Emmert-Buck, M.R., Bonner, R.F., Smith, P.D., Chuaqui, R.F., Zhuang, Z., Goldstein, S.R., Weiss, R.A., and Liotta, L.A. 1996. Laser capture microdissection. Scien
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