Navigation Links
Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

t. #LCM1104)
◊ Capsure HS LCM caps (Arcturus, Cat. #LCM0214)

IV. RNA PURIFICATION, AMPLIFICATION, ASSESSMENT OF QUALITY/YIELD
◊ PicoPure RNA Isolation Kit (Arcturus, Cat. #KIT0204)
◊ RNase-free DNase Set (Qiagen, Cat. #79254)
◊ RiboAmp HS RNA Amplification Kit (Arcturus, Cat. #KIT0205)
◊ Bioanalyzer 2100 (Agilent Technologies)
◊ NanoDrop ND-1000 Spectrophotometer

V. TWO-COLOR cDNA MICROARRAY LABELING, HYBRIDIZATION
◊ Glass cDNA microarrays for the species of interest (made in-house, printed on Corning slides and hybridized using the Corning Pronto protocol)
◊ Corning Pronto Plus Microarray System (Promega Cat. #40056)
◊ All other reagents are identical to Arcturus Protocol #3. Visit www.arctur.com for the complete Protocol #3.

VI. IN SITU HYBRIDIZATION
◊ Gene-specific probes
◊ pCRII TOPO cloning kit (Invitrogen Cat. #K460001)
◊ p33-labeled dUTP (Amersham)
◊ Cold formaldehyde (Sigma)
◊ DEPC-treated PBS (Ambion Cat. #9625)
◊ Triethanolamine (Sigma cat. #T0449)
◊ Acetic anhydride (Sigma cat. #45830)
◊ SSC buffer (Sigma cat. #S8015)
◊ RNase A (Ambion Cat. #2271)
◊ X-ray film emulsion (Kodak)

VII. REVERSE TRANSCRIPTION AND TAQMAN QUANTITATIVE PCR
◊ RETROscript Kit (Ambion, Cat. #1710)
◊ Gene-specific 6-FAM, MGB-labeled probes and sequence detection primers
◊ Taqman Master Mix (Applied Biosystems, Cat.#4304437)
◊ ABI Prism Optical Reaction Plate (Applied Biosystems, Cat. #4314320)

Additional lab supplies and equipment needed:
◊ Surgical instruments '"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12

Related biology technology :

1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
2. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
3. Working With Laser Capture Microdissection Samples
4. Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions
5. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
6. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
7. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
8. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
9. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
10. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
11. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells

Post Your Comments:
(Date:11/5/2009)... , Fuel economy ratings for the new 2010 model ...onomy.gov web site, which Oak Ridge National Labo...ment of Energy and the U.S. Environmental Protecti... fuel economy list are hybrid electrics, led by th...st proves that hybrids have gone mainstream," said...
(Date:11/5/2009)...Call/ -- Imagenetix, Inc. (OTC Bulletin Board: IAG...t and CEO of Imagenetix, will be presenting the Co...e on Thursday, December 3rd, at 10:30 AM PST at th... ,, LD MICRO is a by-invitation only newsletter...s in the micro-cap space. Since 2002, the firm has...
(Date:11/5/2009)...FirstCall/ -- BioSpecifics Technologies Corp. (Nas...ng first-in-class collagenase-based products, toda...arter ended September 30, 2009. ,, "We were ex... FDA,s Arthritis Advisory Committee unanimously re...LEX(TM) for the treatment of Dupuytren,s disease, ...
(Date:11/5/2009)...-FirstCall/ -- WaferGen Biosystems, Inc. (OTC Bull...-the-art genetic analysis systems, today announced...any,s Scientific Advisory Board. His career has b... molecular diagnostics for cancer detection and ot...ational biology systems for several biotechnology ...
Breaking Biology Technology:November 2009 story tips from the US Department of Energy's Oak Ridge National Laboratory 2November 2009 story tips from the US Department of Energy's Oak Ridge National Laboratory 3Imagenetix to Present at 2nd Annual LD MICRO Conference at the Luxe Sunset Bel Air 2BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 2BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 3BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 4BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 5WaferGen Names Christopher Sears, Ph.D., Architect of High-Value Molecular Diagnostics and Computational Biology Platforms, to Company's Scientific Advisory Board 2WaferGen Names Christopher Sears, Ph.D., Architect of High-Value Molecular Diagnostics and Computational Biology Platforms, to Company's Scientific Advisory Board 3WaferGen Names Christopher Sears, Ph.D., Architect of High-Value Molecular Diagnostics and Computational Biology Platforms, to Company's Scientific Advisory Board 4
...ndsen, the managing director of the Wisconsin Alu...term on the national Patent Public Advisory Commit...the U.S. Patent and Trademark Office in its proc...out about his appointment through a letter in Dece... Now a part-time federal government employee, he i...
...lans to hit the markets with an IPO in 2005 or bey...latest technology and your other corporate assets ...tential IPO, your corporate image and brand name r...ut in the marketplace. Both are critical for real ...and the way their unique name played out is a clea...
...hone provider serving areas of Wisconsin, Minnesot...on to phones through the MobiTV network , a produ... $8.59 per month and provides access to 21 channel...s and cartoons as well as the Discovery Channel, C...ypically use the service for 10 to 15 minutes at a...
Other Biology Technology:WARF director to advise U.S. patent office 2Is corporate branding key for IPOs? 2Is corporate branding key for IPOs? 3Midwest Wireless adds live TV to list of cellular services 2
(Date:11/4/2009)... St. Louis, MO, November 4, 2009 Childhood obesit...tions. With more than one fourth of advertising on...s and beverages and continuing questions about the...November/December issue of the Journal of Nutriti...tising aimed at children might be a large contribu...
(Date:11/4/2009)... The University of Oklahoma at Norman has received...e funding agencies as part of the federal stimulus...ding received by OU researchers to $23 million. T...research ranging from archaeology to weather. , ...ematic approach to matching areasof scholarly exce...
(Date:11/4/2009)..., REDWOOD CITY, Calif., Oct. 20 ...ntity and authentication solutions, today announce...nt-of-sale (POS) software for quick-service restau...to their NextGen POS and Classic POS solutions. WA...nt biometrics to boost cashier accountability and ...
Breaking Biology News(10 mins):TV bombards children with commercials for high-fat and high-sugar foods 2TV bombards children with commercials for high-fat and high-sugar foods 3OU achieves $10 million in stimulus grants for 33 projects on the Norman campus 2OU achieves $10 million in stimulus grants for 33 projects on the Norman campus 3WAND Point-of-Sale Solutions Integrate U.are.U(R) Fingerprint Biometrics From DigitalPersona for Quick-Service Restaurants 2WAND Point-of-Sale Solutions Integrate U.are.U(R) Fingerprint Biometrics From DigitalPersona for Quick-Service Restaurants 3Cascades Launches Information Campaign on Hand Drying 60316 1Cascades Launches Information Campaign on Hand Drying 60316 2Cascades Launches Information Campaign on Hand Drying 60316 3Childrens Hospitals and Clinics of Minnesota Announces Scott Leitz as New Director of Child Health Policy and Advocacy 60312 1Childrens Hospitals and Clinics of Minnesota Announces Scott Leitz as New Director of Child Health Policy and Advocacy 60312 2Dendreon Announces Webcast Presentations at Upcoming Investor Conferences 14644 1
...allenges of adapting to climate change as a warmer...food and navigate. To help them adapt, the ESA-bac...atellite-based snow maps. , "Snow is of paramount...ty determines whether reindeer are able to access ...ear," said Anders Oskal, the Director of the Inter...
...tients who smoked, drank, didn,t exercise or didn,...rse survival outcomes than those with better healt...sity of Michigan Comprehensive Cancer Center. , "...rs and genes that might be linked to cancer surviv...ay a major role in his or her survival," says stud...
... , , , , , , , ...report finds that man-made toxic chemicals used as...n all US coastal waters and the Great Lakes. The c... more information. , , , , ,... , , , , , , , NOAA scientists, i...
Other Biology News:Satellite snow maps help reindeer herders adapt to a changing Arctic 2Health choices predict cancer survival, U-M study finds 2NOAA report calls flame retardants concern to US coastal ecosystems 2NOAA report calls flame retardants concern to US coastal ecosystems 3NOAA report calls flame retardants concern to US coastal ecosystems 4
Custom siRNA, HPLC purified from Ambion
Rabbit Anti-MPV17 Polyclonal Antibody, Unconjugated from Proteintech Group, Inc.
GEL FILTRATION CAL KIT HMW from GE Healthcare, formerly Amersham Biosciences
...vides the premium quality siRNAs you need for your...dily ordered online by providing either the 19-21 ...d antisense siRNA sequences. (Also check out Ambio...signed siRNAs, which are already designed for you....
Biology Products: