( t. #LCM1104) ◊ Capsure HS LCM ...)Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

IV. RNA PURIFICATION, AMPLIFICATION, ASSESSMENT OF QUALITY/YIELD
◊ PicoPure RNA Isolation Kit (Arcturus, Cat. #KIT0204)
◊ RNase-free DNase Set (Qiagen, Cat. #79254)
◊ RiboAmp HS RNA Amplification Kit (Arcturus, Cat. #KIT0205)
◊ Bioanalyzer 2100 (Agilent Technologies)
◊ NanoDrop ND-1000 Spectrophotometer

V. TWO-COLOR cDNA MICROARRAY LABELING, HYBRIDIZATION
◊ Glass cDNA microarrays for the species of interest (made in-house, printed on Corning slides and hybridized using the Corning Pronto protocol)
◊ Corning Pronto Plus Microarray System (Promega Cat. #40056)
◊ All other reagents are identical to Arcturus Protocol #3. Visit www.arctur.com for the complete Protocol #3.

VI. IN SITU HYBRIDIZATION
◊ Gene-specific probes
◊ pCRII TOPO cloning kit (Invitrogen Cat. #K460001)
◊ p³³-labeled dUTP (Amersham)
◊ Cold formaldehyde (Sigma)
◊ DEPC-treated PBS (Ambion Cat. #9625)
◊ Triethanolamine (Sigma cat. #T0449)
◊ Acetic anhydride (Sigma cat. #45830)
◊ SSC buffer (Sigma cat. #S8015)
◊ RNase A (Ambion Cat. #2271)
◊ X-ray film emulsion (Kodak)

VII. REVERSE TRANSCRIPTION AND TAQMAN QUANTITATIVE PCR
◊ RETROscript Kit (Ambion, Cat. #1710)
◊ Gene-specific 6-FAM, MGB-labeled probes and sequence detection primers
◊ Taqman Master Mix (Applied Biosystems, Cat.#4304437)
◊ ABI Prism Optical Reaction Plate (Applied Biosystems, Cat. #4314320)

Additional lab supplies and equipment needed:
◊ Surgical instruments '"/>

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