Navigation Links
Lactococcus lactis

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.519 12/2001 Microorganism Lactococcus lactis MG1363 Cell type Bacteria, gram positive Molecules injected Plasmid DNA (pGK12) Growth medium Complex medium with 1% glycine Washing solution 0.5 M sucrose, 10% glycerol Electroporation solution 0.5 M sucrose, 10% glycerol Outgrowth medium Ice-cold complex medium with 0.5 M sucrose, 20 mM MgCl2, 2 mM CaCl2 Cuvette 1 mm gap width Reference Dr. Horst Neve Bundesanstalt fr Milchforschung Institut fr Mikrobiologie Hermann-Weigmann Str. 1 D-24103 Kiel Phone +49 431 6091 Fax +49 431 609222 Making electrocompetent cells:

1. Grow cells overnight at 30 C to an O.D.620 of 0.7. 2. Wash twice with ice-cold washing solution. 3. Resuspend cells in 1/100 volume of electroporation solution. Keep on ice.

Electroporation of
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Lactococcus lactis
2. Kluyveromyces lactis
3. Kluyveromyces lactis

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Lactococcus lactis

(Date:11/24/2009)..., REYKJAVIK, Iceland, November 24 /PRNewswire... ) today announced that it has received notice fro...any,s common stock will be,suspended as of Novembe...e,Securities and Exchange Commission, which will r..., unless the company files an appeal to the Nasdaq...
(Date:11/24/2009)..., REYKJAVIK, Iceland, November 24 /PRNewswire... ) today announced the approval of all of its,"fir...he District of Delaware,(the "Bankruptcy Court"). ...lief under Chapter 11 of the United States Bankrup... orders issued by the Bankruptcy Court allow the c...
(Date:11/24/2009)...HANGHAI, Nov. 24 /PRNewswire-Asia/ -- Shanghai Bio...dministration (SFDA) has approved the,investigatio...n project,(Nodlin), an innovative insulin formulat...olaxy to initiate its first phase I clinical study... of high blood glucose and poor,metabolism. The co...
(Date:11/24/2009)...ary efficacy endpoint has been met for patients wi...cer, , QUEBEC CITY, Nov. 24 /PRNewswire-First...: AEZ) (the "Company"), a global biopharmaceutical..., today announced positive efficacy data from a Ph...njugate, AEZS-108 (formerly AN-152), in patients w...
Breaking Biology Technology:deCODE Receives Delisting Notice From Nasdaq, Plans to Appeal 2deCODE Receives Delisting Notice From Nasdaq, Plans to Appeal 3deCODE genetics, Inc. Announces Approval of First Day Motions by Bankruptcy Court 2deCODE genetics, Inc. Announces Approval of First Day Motions by Bankruptcy Court 3Biolaxy Secures IND Approval for Oral Insulin 2AEterna Zentaris Announces Positive Results for Phase 2 Study with LHRH-Receptor Targeted Cytotoxic Conjugate AEZS-108 in Endometrial Cancer 2AEterna Zentaris Announces Positive Results for Phase 2 Study with LHRH-Receptor Targeted Cytotoxic Conjugate AEZS-108 in Endometrial Cancer 3AEterna Zentaris Announces Positive Results for Phase 2 Study with LHRH-Receptor Targeted Cytotoxic Conjugate AEZS-108 in Endometrial Cancer 4
...GE, Tenn., Jan. 17, 2008 Scientific studies on cl...ng the 30 projects awarded more than 145 million p...ional Laboratory through the Department of Energys...ry and Experiment (INCITE) program. , Through I...ernment research facilities receive access to comp...
..., Holder Completes First Year Inspiring Others to ...tions, VALLEY FORGE, Pa., Jan. 17 In 2007, Good ... teamed up with "Iron Andy" Holder,as part of the ...n. Andy,set out to speak about living with diabete...ted with dozens of individuals who were inspired b...
...Previous Guidance for 2007, MADISON, N.J., Jan. 1...he nation,s leading provider of diagnostic,testing... report results,for the fourth quarter and year en...ce for 2008 on February 21, 2008, instead of the p...any is rescheduling its report of fourth quarter a...
Other Biology Technology:Oak Ridge leads DOE INCITE effort in 2008 2Oak Ridge leads DOE INCITE effort in 2008 3Oak Ridge leads DOE INCITE effort in 2008 4Good Neighbor Pharmacy(R) Ironman Competitor Inspires Individuals with Diabetes to Live Without Limits 2Good Neighbor Pharmacy(R) Ironman Competitor Inspires Individuals with Diabetes to Live Without Limits 3Quest Diagnostics Sets New Date to Report Fourth Quarter and Full Year 2007 Results 2Quest Diagnostics Sets New Date to Report Fourth Quarter and Full Year 2007 Results 3
(Date:11/24/2009)...s Research Institute (LRI) today named 12 new gran...illion, recognize innovative work across a broad s...heir creativity, novelty, and potential to drive s...and cure the complex disease of systemic lupus. ,...upus researchers across the nation and include int...
(Date:11/24/2009)...ic-level action of a remarkable class of ring-shap...s with the Lawrence Berkeley National Laboratory (...stallography beamline at the Advanced Light Source... gene expression and replication, and are vital to...infectious agents, such as the human papillomaviru...
(Date:11/24/2009)...nstitutes fish food is a matter of debate. A high-...get almost 50 percent of their carbon from trees a...the terrestrial and aquatic ecosystems. , But ne... this is not likely to be true. Algae provide a mu...cording to research published this week in the Pr...
Breaking Biology News(10 mins):Lupus Research Institute announces 2009 novel research grants 2Lupus Research Institute announces 2009 novel research grants 3Atomic-level snapshot catches protein motor in action 2Atomic-level snapshot catches protein motor in action 3Atomic-level snapshot catches protein motor in action 4Fish food fight: Fish don't eat trees after all, says new study 2Fish food fight: Fish don't eat trees after all, says new study 3WuXi PharmaTech Files 2008 Annual Report on Form 20 F 50138 1WuXi PharmaTech Files 2008 Annual Report on Form 20 F 50138 2High carbon dioxide levels cause abnormally large fish ear bones 9012 1High carbon dioxide levels cause abnormally large fish ear bones 9012 2SEIU UHW Members Win Key Vote to Keep Union at Calif Hospital 50136 1SEIU UHW Members Win Key Vote to Keep Union at Calif Hospital 50136 2
...chologists have long known that memories of distur... or the unexpected death of a loved one - are more...ane recollections of everyday matters. , Probing ... the University of Wisconsin-Madison have found th...n fire up two memory-forming regions of the brain ...
...om from a West Indian tarantula has been shown to ...e that sense high temperatures and the hot, spicy ...discovered. , The findings demonstrate that some p...strategy to deter predators -- triggering pain by ...The research provides new tools to understand how ...
... gardener knows--different plant species mature at...ities in natural habitats call this phenomenon "co... because it reduces overlap in the time period whe... study posted online the week of Sept. 4 in the Pr...logists working at Stanford,s Jasper Ridge Biologi...
Other Biology News:Anticipation plays a powerful role in human memory, brain study finds 2Anticipation plays a powerful role in human memory, brain study finds 3Tarantula venom and chili peppers target same pain sensor 2Global changes alter the timing of plant growth, scientists say 2Global changes alter the timing of plant growth, scientists say 3
Bacterial Strain NM522, Glycerol Stock from Promega
Mounting Medium from R&D Systems
Cultrex BME with phenol red from Trevigen
Human Erythropoietin, Unconjugated from R&D Systems
Biology Products: