Navigation Links
LC-MALDI and MALDI-MS/MS for the Analysis of Complex Peptide Mixtures

Joseph Fox1, Joanne B. Connolly2, Matthew E. Openshaw2
1Shimadzu Scientifi c Inc., Columbia, US; 2Shimadzu Biotech/Kratos, UK


Introduction
LC-MALDI provides several advantages over conventional single spot (i.e. no separation) acquisitions for the analysis of complex peptide mixtures. These include (i) concentration and separation of the peptides prior to analysis, (ii) extended time available for MS/MS interrogation, (iii) separation of isobaric peptides resulting in increased sequence coverage. The ability to separate and concentrate the peptides prior to analysis results in higher sensitivity and means that more MS/MS spectra can be generated from the sample. Rather than acquiring all the MS/MS spectra from the same spot, with the sample being depleted with each MS/MS spectrum acquired, MS/MS spectra are generated from different positions on the MALDI target and sample depletion is less of an issue.

In the LC-MALDI technique, the protein mixture is enzymatically digested and the resulting peptides are separated prior to MALDI analysis (see Figure 1). Automated MALDI time-of-fl ight (TOF) mass spectrometric (MS) analysis is used to select suitable candidates for subsequent MS/MS fragmentation and proteins are identifi ed using the MS/MS data obtained for the individual peptides. We present results obtained using a new MALDI-TOF mass spectrometer, capable of true high-energy MS/MS (AXIMA-TOF2), demonstrating the utility of this technique for the analysis of complex protein mixtures. Also presented is data obtained for an iTRAQ labelled protein mixture, including a novel MALDI-MS/MS analysis mode (Low Mass Zoom (LMZ)) which can be used to enhance fragment ion resolution for low mass fragment ions, e.g. diagnostic ions used for relative quantifi cation (iTRAQ
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: MALDI and MALDI for the Analysis Complex Peptide Mixtures

(Date:12/17/2014)... (PRWEB) December 17, 2014 Gene ... that it has entered into a technology access ... (ADM) to apply DNA2.0’s proprietary protein engineering technology, ... process. , “We are extremely excited that the ... platform. This proprietary bioengineering technology has now ...
(Date:12/17/2014)... SOUTH SAN FRANCISCO, Calif. , Dec. 17, ... positive results of a Phase 2 study evaluating ... for the treatment of patients with severe, chronic ... the current standard of care, including topical steroids ... endpoint was percent change in Visual Analog Scale ...
(Date:12/15/2014)...  GlassesOff Inc. (OTCBB: GLSO) announced today the appointment ... director of the Company,s Board of Directors. ... its CEO until its acquisition by Stanley Black ... Recognized as the inventor of the first Wi-Fi-based Active ... -based RFID solutions focused on improving operational efficiency, safety ...
(Date:12/13/2014)... (PRWEB) December 12, 2014 Clarassance, a ... announced its new name: Therabron Therapeutics , Inc. ... and bronchioles (a type of structure in the lungs ... company’s mission to develop novel protein therapeutics for the ... directors decided to change the name to mark the ...
Breaking Biology Technology:ADM and DNA2.0 Enter Into Protein Engineering Technology Access and Service Agreement 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 3Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 4AeroScout Founder Joins GlassesOff's Board of Directors 2AeroScout Founder Joins GlassesOff's Board of Directors 3Maryland-based Biotech Company's Path Forward in Treating Respiratory Diseases Sparks Name Change 2
... Sonia Moralejo Garca at the University of the Basque Country ... and other magnetic devices of wide industrial application. , It ... that are ever smaller and that nanotechnology, although it its ... in our society. Who has not heard of nanotubes, nanoparticles ...
... INCREASE 14.6%. Q4 2007 DILUTED EPS UP 20.8% TO $.64. 2008 ... $2.62 PER SHARE., ST. LOUIS, Feb. 12 , HIGHLIGHTS: ... -- Q4 2007 Sales Set New Quarterly Record: At $532.1 ... high, beating the Q2 2007 record of $507.5 ...
... of Dow AgroSciences Announces Milestone in Wall Street ... ,Dow AgroSciences LLC and Sangamo BioSciences, Inc. (Nasdaq: ... a key research milestone as part of,their joint ... successful application of Sangamo,s zinc finger DNA-binding,protein nuclease ...
Cached Biology Technology:A researcher of UPV/EHU has designed nanomagnets for industry 2Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 2Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 3Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 4Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 5Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 6Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 7Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 8Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 9Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 10Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 11Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 12Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 13Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 14Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 15Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 16Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 17Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 18Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 19Sigma-Aldrich (Nasdaq: SIAL) Annual Sales Surpass $2 Billion Mark. 20Dow AgroSciences, Sangamo BioSciences Announce the Achievement of Key Milestone in Plant Agriculture Collaboration 2Dow AgroSciences, Sangamo BioSciences Announce the Achievement of Key Milestone in Plant Agriculture Collaboration 3Dow AgroSciences, Sangamo BioSciences Announce the Achievement of Key Milestone in Plant Agriculture Collaboration 4
(Date:12/10/2014)... , Dec. 9, 2014  Valencell, a leader in ... seeing a staggering demand from its licensees for highly ... revealed, is not solely coming from fitness and health ... "A wearable is only as useful as ... is the ultimate driver in long-term mass consumer adoption ...
(Date:12/10/2014)... 08, 2014 Research and Markets ... the "Biometrics Market in Japan 2014-2018" ... http://photos.prnewswire.com/prnh/20130307/600769 The integration of ... rural banking and upgradation of the driver,s license ... the market. Besides the aforementioned projects, biometrics is ...
(Date:12/5/2014)... , Dec. 4, 2014  Tute Genomics, a leader ... million in Series A1 funding led by UK-based Eurovestech. ... participated in the investment round. "We are ... community adopts next-generation sequencing and seeks new approaches for ... Reid Robison , MD MBA, and CEO of Tute ...
Breaking Biology News(10 mins):Wearable Technology Products Demand Highly Accurate Biometric Technology 2Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3
... the northwest coast of North America is reaching the level ... study led by a researcher at the University of British ... Marine Pollution Bulletin , examined stomach contents of beached northern ... states of Washington and Oregon, U.S.A. "Like the canary ...
... 3, 2012 From solar energy to water treatment, ... "sustainability" is more than just a buzzword. ... Council of Canada (NSERC) announced that Concordia had received ... an Institute in Water, Energy and Sustainability. Not only ...
... COLUMBIA, Mo. The most common ailment to affect a ... has developed a way to detect this problem using a ... Keegan, a professor of equine surgery in the College of ... can detect lameness earlier than veterinarians using the traditional method ...
Cached Biology News:Seabirds study shows plastic pollution reaching surprising levels off coast of Pacific Northwest 2$1.6 million for sustainability 2Motion sensors detect horse lameness earlier than veterinarians, MU study finds 2
RABBIT ANTI HUMAN TRRAP (CT) Immunogen: Recombinant C-terminal kinase-related domain of human TRRAP....
...
SHEEP ANTI DEHYDROEPIANDROSTERONE...
... by N.C. Price (1996) • ... on protein chemistry and the ... proteins. Data on the following ... size determination, amino acid analysis ...
Biology Products: