Navigation Links
L2

oncentration to 2 x 106 cell/ml.
  • Add and mix plasmid DNA (15 g/ml final concentration, in bidistilled H2O).
  • Transfer 400 l cell suspension into electroporation cuvettes (2 mm gap width). The cell suspension must be free of air bubbles.
  • Electroporation:

    Mode Eukaryotes Voltage (V) 180 V Time constant (T) 100 s No. of pulses (n) 1
  • After the pulse, allow the cell suspension to stand in the cuvette for 10 minutes at room temperature.
  • Carefully transfer the cell suspension from the cuvette into 3 ml M3 / 5% FCS, and cultivate them in a 35 mm culture dish of a 6-well plate.

    Detection methods for transfection:

    The expression of EGFP driven by the insect actin promotor can be detected clearly after 24-48 hours by FACS analysis
    or under a fluorescence microscope. Result: Survival rate: 70-80% Transfection rate (stable): 6-9% based on the number of surviving cells. Results were measured 24-48 hours after transfection.


    '"/>


  • Source:


    Page: All 1 2

    Related biology technology :

    1. Kluyveromyces lactis
    2. Lactococcus lactis
    3. Lactococcus lactis
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG:

    (Date:8/23/2014)... Rochelle, Virginia (PRWEB) August 23, 2014 ... of NDA Partners LLC announced today that Janet ... a Premier Expert consultant. NDA Partners Premier Experts ... stature enable them to bring extraordinary value to ... and implement critical solutions to help clients successfully ...
    (Date:8/22/2014)... August 23, 2014 This is ... state of the Papain industry in Global and ... the industry, including definitions, applications and industry chain ... analysis are provided with a focus on history, ... A comparison between the international and Chinese situation ...
    (Date:8/22/2014)... Muncie, IN (PRWEB) August 22, 2014 Today ... Review in the United States Court of Appeals for the ... for Model Aircraft published in the Federal Register on June ... Interpretive Rule is contrary to the text and intent of ... 112-95, and violates Congress’ specific prohibition on any new rules ...
    (Date:8/22/2014)... Aug. 22, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the ... ended June 30, 2014.  Biorem,s complete 2014 second quarter financial statements and ... Summary:Three-months ended June 30, , Six-months ended June ... , 2013 , 2014 , 2013 ... , 9,195 Gross profit , 56 , ...
    Breaking Biology Technology:Noted Epidemiologist and Former FDA Deputy Director Janet B. Arrowsmith, MD, Joins NDA Partners as a Premier Expert Consultant 2Papain Industry Global & China Development Trend and Proposals Analysis Research Report Available at DeepResearchReports.com 2Papain Industry Global & China Development Trend and Proposals Analysis Research Report Available at DeepResearchReports.com 3AMA Files Petition for Review of FAA’s Interpretive Rule 2Biorem Reports 2014 Second Quarter Results 2Biorem Reports 2014 Second Quarter Results 3
    ... Iceland, and ALAMEDA, California, April 23, deCODE genetics ... CRA ) today announced the signing of agreements ... to deCODE,s,genetic markers for increased risk of major ... fibrillation (AF) and type 2,diabetes (T2D). These markers ...
    ... April 23 Cord Blood America, Inc. (OTC Bulletin ... company ( http://www.cordblood-america.com ) focused ... to families nationwide and internationally, which recently announced it ... said today it has eliminated another $817,000 of long-term ...
    ... honors President Carter, Carter Center for fight against neglected ... Progress (GHP) and ONE, as well as members of ... foundations and the business community, welcomed a delegation of ... reception on Capitol Hill today. The delegation presented a ...
    Cached Biology Technology:deCODE and Celera Partner to Expand use of deCODE Risk Markers for Heart Attack, Stroke and Diabetes 2deCODE and Celera Partner to Expand use of deCODE Risk Markers for Heart Attack, Stroke and Diabetes 3deCODE and Celera Partner to Expand use of deCODE Risk Markers for Heart Attack, Stroke and Diabetes 4deCODE and Celera Partner to Expand use of deCODE Risk Markers for Heart Attack, Stroke and Diabetes 5deCODE and Celera Partner to Expand use of deCODE Risk Markers for Heart Attack, Stroke and Diabetes 6deCODE and Celera Partner to Expand use of deCODE Risk Markers for Heart Attack, Stroke and Diabetes 7Cord Blood America Further Reduction, $817,000, in Long-Term Debt 2African Health Delegates Welcomed to Washington 2
    (Date:8/22/2014)... , August 22, 2014 ... ) has announced the addition of ... 2014-2018"  report to their offering.  ... ,Biometrics is a technology that helps ... on their physical or behavioral features. ...
    (Date:8/22/2014)... a leading source of information for businesses and professionals, ... Riverside in its 2014 list of "some of the ... generate the list, the company analyzed citation data over ... has had enormous impact. , The following UC Riverside ... the greatest number of highly cited papers in one ...
    (Date:8/22/2014)... at the 29th Annual International Papillomavirus Conference shows ... Inc. combats three types of human papillomavirus, including ... cervical cancer. , When tested in several cultured ... HPV-16, HPV-18 and HPV-11 cells, according to Louise ... presented the findings at the Seattle conference. The ...
    Breaking Biology News(10 mins):Biometrics Market in North America 2014-2018: Key Vendors are 3M Cogent, Cross Match, NEC and Safran SA 2Biometrics Market in North America 2014-2018: Key Vendors are 3M Cogent, Cross Match, NEC and Safran SA 35 UC Riverside scientists among world's most influential scientific minds 2
    ... filled with widespread species, while moderate and higher elevations were ... is turning out to be partially incorrect. Widespread species now ... new study describes four species once considered to be the ... Plica plica . The study was published in ...
    ... researchers found that chicken eggs can provide a better ... professor in Clemson,s College of Agriculture, Forestry and Life ... Research Excellence award that allowed researchers to identify two ... congenital deafness and spine deformities in humans. Chapman ...
    ... turns out that your messenger RNA may catch more than one ... mRNA may travel one way down a cell, then board another, ... get where it ultimately needs to be. It,s a ... differentiating a brain cell from, say, a muscle cell, said ...
    Cached Biology News:The collared treerunner is more than a single species 2Clemson research finds chickens offer clues to human birth defects 22-way traffic enable proteins to get where needed, avoid disease 2