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Kodak in vivo imaging system: precise coregistration of molecular imaging with anatomical X-ray imaging in animals

ts of the animal paw (Fig. 2b), and the overlay image demonstrates the expected colocalization and the precise coregistration of these two modalities in the Kodak instrument (Fig. 2c).

Combined multiwavelength fluorescence and X-ray imaging is shown in Figure 3. We injected three different fluorescently tagged imaging agents subcutaneously into different regions of the mouse abdomen. Fluorescence imaging with different filter sets appropriate for each fluorochrome was followed by X-ray imaging. The four images, representing the three different fluorescent channels and the X-ray image, were easily contrasted and pseudocolored in Kodak MI 4.0 software and merged in Adobe Photoshop.

The image in Figure 4 demonstrates the combination of radioisotope 18F imaging (typically used in positron emission tomography (PET) imaging) with X-ray imaging. The mouse was injected with [18F] fluordeoxyglucose (FDG) PET and imaged with the Kodak Image Station In-Vivo FX using the radioisotopic imaging screen for 8 min with the camera in the highest-binning state. The image was contrasted and overlayed on the subsequent X-ray image to show the localization of the isotope in the heart of the animal.


Conclusion
Kodak has developed and commercialized powerful multimodal in vivo imaging systems that greatly enhance the localization of molecular signals in live animals. These systems are now used by top academic, biotechnology and pharmaceutical research institutes worldwide. The flexibility of the system allows the combination and coregistration of multiple wavelengths and multiple modalities of
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