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Kodak in vivo imaging system: precise coregistration of molecular imaging with anatomical X-ray imaging in animals

multi-wavelength fluorescence, luminescence and radioisotopic imaging in small animals. The In-Vivo FX includes all of the capabilities of the In-Vivo F and the high-resolution X-ray Imaging Module using a Radiographic (X-ray) Imaging Screen.

For both radiographic and radioisotopic imaging, patented Kodak phosphor screens coupled to speed-enhancing interference optics efficiently convert the ionizing radiation into light. The light is emitted by the screens and captured by the charge-coupled device (CCD) camera to form the image. Two different screen assemblies are available. One is optimized for the high-energy radioisotopes such as 111In, 99Tc and 18F, and the other is optimized for the low-energy, high-resolution requirements of X-ray imaging.


Multimodal imaging operation
The Kodak Image Station In-Vivo F and FX systems use the same operation and hardware for optical imaging of an animal (or multiple animals) immobilized and positioned in the animal chamber directly above the imaging chamber window (Fig. 1a). For fluorescence, excitation light from a high-intensity lamp is directed through the selected excitation filter to the animal. Fluorescence from the imaging agent inside the animal is then emitted and separated from the excitation light as it passes through the patented Kodak Wide Angle emission filter. The fluorescence enters the 10 zoom lens and is focused onto a 4 million pixel, cooled CCD. The digitized readout is efficiently interfaced to a personal computer (Windows or Mac).

Multiple optical images of different molecular entities with different fluorescent tags can be captured in the same animal by si
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