Kluyveromyces lactis ( Electropora...)

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Kluyveromyces lactis

Electroporation of cells:

Add 5 l DNA (1 g/l) to 100 l of electrocompetent cells. Homogenize by gently mixing with pipette several times. Transfer mixture into a prechilled cuvette.
Wipe moisture from the cuvette and insert the cuvette into the device.
Electroporation:

Mode Prokaryotes O Voltage (V) 2,500 V Time constant (T) 5 ms
Add 1 ml YPD medium with 1 M sorbitol, incubate for 1 hour at 30 C.
Centrifuge cells 30 sec., resuspend in 0.4 ml sterile water, plate in aliquots on selective plates.

Expected results: Transformation efficiency up to 200 transformants/g of DNA.

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Related biology technology :

1. Kluyveromyces lactis
2. Lactococcus lactis
3. Lactococcus lactis

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