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Kinetic determination of vitamin B1

owers students analyzed five types of commercial vitamin tables using a simplified technique: samples were run at room temperature without stirring, the reaction was followed on a recorder, and rates were determined by hand from the plots. Typical results included determinations within 2% of the stated amount of thiamine in a therapeutic type, within 5% in a chewable type, and within 6% in a one-a-day type. Replication was within 5% RSD for tablets from the same bottle and for preparations of the same type by different manufacturers.

Through their development of a highly sensitive and selective technique for thiamine determinations, Ryan and Ingle have demonstrated the applicability of kinetic fluorescence to the analytical laboratory. Bowers experiments emphasize the simplicity and repeatability of the technique.

Because it offers sensitive fluorescence measurements, full system control, extensive graphics and data-processing functions and automated keystroke programming, the FLUOROLOG spectrofluorometer is ideal for kinetic fluorescence determinations. The calibration procedure described herein can be applied readily to reaction-rate determinations with your FLUOROLOG system.


1 Ryan, M.A; Ingle, J.D. Anal. Chem. 1980, 52, 21772184.
2 Bower, J. J. Chem. Ed. 1982, 59, 975977.


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