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Keys to Successful Densitometry

user can change the equation type from the Concentration Calibration menu until the equation that best fits the data is displayed. Determining the best equation is important because the software will use this curve to calculate the concentration of unknown bands or spots. After the standard curve is calibrated, values for samples of unknown concentration can be determined by detecting a band area, again either automatically or manually using the ROI function. Integrated density and concentration information can then be added to the image overlay, stored with the image and printed (figure 1 (30k),, RED and GREEN).

Conclusions

Eagle Sight software version 3.0 can be used to determine integrated density and concentration information from images of stained gels or other laboratory experiments. By ensuring that pixels within bands or spots of interest are not saturated during acquisition and that background levels and analysis areas are appropriately defined prior to analysis, researchers will be able to increase the accuracy and consistency of densitometry results.

REFERENCES

  1. Steege, M., and Cayouette, M. (1996) Strategies 9: 53-56.
  2. Cayouette, M., and Hansen, C. (1996) Strategies 9: 56-57.

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