Navigation Links
Keys to Successful Densitometry

ed, relative to nonsaturated samples. Therefore, spots or bands containing saturated pixels should not be used for precise densitometry analysis.

Background: The Integrated Density function sums all pixel values, minus a background value for each pixel, within an area of defined dimensions. Small differences in pixel value, even a value of 1 or 2, summed over an entire area can have a large cumulative effect on integrated density calculations. For this reason, care should be taken to have background values roughly equivalent throughout the analysis area and to properly define the background. Accurate densitometric analysis of images whose background levels vary greatly within analysis areas is difficult. In general, before beginning the analysis, the user should define the background level from an area of the image closest to the bands or spots of interest. This will ensure that the appropriate background value is subtracted from every pixel within the analysis area. If the user does not define the background, the software will automatically select the most common pixel value (the modal value) within the entire image and use this value for all background measurements.

Defining the background value before analysis can be accomplished either manually or automatically using the Set Background function located in the Analyze menu. By choosing the automatic option, users can define an area that they consider to be representative of background levels near bands of interest. Typically, this area will be right above or below the bands to be analyzed. In order to visualize background levels more readily, the False Color option from the Process menu can be used. By replacing the gradual grayscale with sharp differences in color, the False Color option displays small differences in pixel value more readily and allows the background threshold levels to be defined such that the most accurate results are pro
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Successful PCR amplification and subcloning of a GC-rich DNA fragment
2. General Considerations for Successful Transfection Experiments
3. Designing a Successful qRT-PCR Experiment
4. Precursor miRNAs for Successful miRNA Functional Studies
5. Recommendations for Successful siRNA Library Screens
6. Setting up Successful siRNA Library Screens
7. Successful stabilization of gene expression profiles
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Keys Successful Densitometry

(Date:10/30/2014)... 2014 SoundConnect , an ... Office 365 end users the key to turning ... system by adding voice enablement and hybrid audio ... Online, a communication tool that includes presence, instant ... voice, O365 becomes a comprehensive unified communications solution. ...
(Date:10/30/2014)... October 30, 2014 James Sherley says he ... adult tissue stem cell technology since his days as a ... Philadelphia in the late 1990’s. Sherley founded the ASCTC ... company holds all the intellectual property developed in Sherley’s research ... the Massachusetts Institute of Technology (MIT), and more recently as ...
(Date:10/30/2014)... New York, NY (PRWEB) October 30, 2014 ... investment plan for its ICH Stability Testing and Package ... companies, this investment centers on a dramatic increase in ... larger volume of stability, storage and aging samples to ... first stage of this major capital expenditure has already ...
(Date:10/30/2014)... Grace Century portfolio project, Provia Laboratories ... of the Board, to the National Stem Cell Ethics ... made by the Ministry of Health on behalf of the ... , More than a year after Bahamian Parliament passed the ... Perry Christie said the government has completed the accompanying regulations ...
Breaking Biology Technology:SoundConnect Launches Voice Enablement and Hybrid Audio Conferencing for Office 365 2SoundConnect Launches Voice Enablement and Hybrid Audio Conferencing for Office 365 3Boston’s Adult Stem Cell Technology Center, LLC Introduces A New Technology for Monitoring Previously Elusive Adult Stem Cells 2Boston’s Adult Stem Cell Technology Center, LLC Introduces A New Technology for Monitoring Previously Elusive Adult Stem Cells 3Boston’s Adult Stem Cell Technology Center, LLC Introduces A New Technology for Monitoring Previously Elusive Adult Stem Cells 4Whitehouse Labs Announces Major Capital Investment in Stability and Storage Testing Capacity 2Whitehouse Labs Announces Major Capital Investment in Stability and Storage Testing Capacity 3Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 2Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 3
... amounts are in U.S. dollars, QUEBEC CITY, May ... TSX: AEZ), a global biopharmaceutical company focused on,endocrinology and ... quarter ended March 31, 2008., First Quarter ... second efficacy trial of the Phase ...
... Calif., May 6 Finesse Solutions, LLC,Santa ... control solutions for,life sciences process applications, announced ... of Software. Basil brings over 19 years ... a focus on,implementing high efficiency engineering, resource ...
... Genta,Incorporated (OTC Bulletin Board: GNTA) announced today ... Regulatory Authority (FINRA, formerly,the NASD) has cleared a ... stock on the Over-the-Counter Bulletin Board (OTCBB). As ... for the Company,s,common stock will begin on Wednesday, ...
Cached Biology Technology:AEterna Zentaris Reports First Quarter 2008 Financial and Operating Results 2AEterna Zentaris Reports First Quarter 2008 Financial and Operating Results 3AEterna Zentaris Reports First Quarter 2008 Financial and Operating Results 4AEterna Zentaris Reports First Quarter 2008 Financial and Operating Results 5AEterna Zentaris Reports First Quarter 2008 Financial and Operating Results 6AEterna Zentaris Reports First Quarter 2008 Financial and Operating Results 7Basil Zimmo Joins Finesse Solutions as Vice-President of Software 2Genta Announces Transfer of its Common Stock to the OTC Bulletin Board 2Genta Announces Transfer of its Common Stock to the OTC Bulletin Board 3
(Date:10/29/2014)... a large cohort of kidney cancer patients in Europe ... -- and reveals an apparent link between exposure to ... Romania. , The research, by an international team led ... Innovation Centre in Montreal, underscores the importance of investigating ... found in plants of the Aristolochia genus, also has ...
(Date:10/28/2014)... (NIH) announced awards to expand the Office of ... for Advancing Translational Sciences (ORDR-NCATS) collaborative Rare Diseases ... physician scientists at 22 consortia will work with ... and investigate new treatments for patients with rare ... million in fiscal 2014 funding from NIH. , ...
(Date:10/28/2014)... and juices are associated with a lower risk of ... University of East Anglia (UEA). , Research published ... and flavanones (both subclasses of dietary flavonoids) significantly decrease ... cause of cancer death among women. , The ... aged between 25 and 55 for more than three ...
Breaking Biology News(10 mins):Study sheds light on genetic architecture of kidney cancer 2Study sheds light on genetic architecture of kidney cancer 3NIH's ORDR-NCATS RDCRN & NICHD awards U54 cooperative agreement for natural history study 2NIH's ORDR-NCATS RDCRN & NICHD awards U54 cooperative agreement for natural history study 3Tea and citrus products could lower ovarian cancer risk, new UEA research finds 2
... WASHINGTON Synthetic biology promises to enable cheap, lifesaving ... suffer from malaria, and to create innovative biofuels that ... the science and its applications are raising questions: ... purposely changing the definition of what is life? ...
... Carolina, there are five strange looking "patches" cleared out ... circles carved by aliens. , They,re actually budding longleaf ... St. Louis and their collaborators at North Carolina State ... Washington have created these ecological patches with the help ...
... French . Plant researchers from ... announced a major breakthrough in a developmental process called ... reversal of what is called epigenetic silencing in plants. ... better understanding of gene regulation in the continuing quest ...
Cached Biology News:Synthetic biology: Is ethics a showstopper? 2Study on wildlife corridors shows how they work over time 2Study on wildlife corridors shows how they work over time 3Study on wildlife corridors shows how they work over time 4Plants display 'molecular amnesia' 2Plants display 'molecular amnesia' 3
... traps a wide variety of solvent vapors that can ... flask (GCF400) allows for the collection of liquid into ... time minimizes waiting period., Ultra low -104 ... , Digital Display of ...
... RCT series of refrigerated cold traps offers ... of low volatility/aqueous solvents or high volatility ... other vacuum concentrators/centrifugal evaporators. Also used for ... such as used in electron microscopes. Can ...
... Trap is specifically designed for efficient ... as DMSO and DMF in the ... refrigeration system set at -5 o ... DMSO, while completely eliminating the risk ...
96 Well Plate Sterile, RE bottom; consistent medium binding affinity for hydrophobic complexes and proteins....
Biology Products: