Navigation Links
Keys to Successful Densitometry

ation

Integrated density is defined as the sum of pixel values minus a background value for each pixel within a bounded area. For a given area, increased brightness or size of a band or spot correlates to higher integrated density measurements. Integrated density measurements are useful for comparing band or spot intensities on images where no samples of known concentration (standards) are included. The Integrated Density function of Eagle Sight software version 3.0 allows rapid comparison between bands or spots within a single image from the Eagle Eye system.

figure 1

Determining integrated density with Eagle Sight software begins with choosing the Integrated Density function from the toolbar or Analyze menu (figure 1 (30k), RED). Integrated density information can then be viewed either by clicking directly on the band or spot or by clicking within a user-defined region of interest. This information can then be written on the image overlay, saved with the image and printed to preserve a hard copy of the final data.

Pixel Saturation: When acquiring an image for integrated density determinations, the user must keep the Saturation Warning function of the View menu enabled to avoid saturating pixels within bands or spots of interest. Enabling the Saturation Warning function results in image pixels with values of 255 to be displayed in red. Because 255 is the largest possible pixel value, increasing the exposure time of an image that already contains saturated pixels will not increase these pixel values further. However, pixel values below 255 will continue to increase with longer exposures until they too become saturated. Integrated density calculations on bands or spots having saturated pixels will produce measurements that are lower than expect
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Successful PCR amplification and subcloning of a GC-rich DNA fragment
2. General Considerations for Successful Transfection Experiments
3. Designing a Successful qRT-PCR Experiment
4. Precursor miRNAs for Successful miRNA Functional Studies
5. Recommendations for Successful siRNA Library Screens
6. Setting up Successful siRNA Library Screens
7. Successful stabilization of gene expression profiles
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Keys Successful Densitometry

(Date:1/15/2014)... The Microcompetition with Foreign DNA theory explains how latent viruses ... is the Epstein Barr Virus (EBV), and one of these ... chronic inflammatory disease that destroys the body’s joints. As predicted ... high concentrations of EBV DNA in their bodies (1). ...
(Date:1/15/2014)... Biotechnology Company, Limited ("TaiGen") today announced that they have ... pharmaceutical company, to develop and commercialize nemonoxacin (Taigexyn ® ... Turkey and other members of ... for the treatment of bacterial infections including those caused ...
(Date:1/14/2014)... 14, 2014 As pet owners drew up their lists of ... wrote: "Take better care of my furry companion." Nowadays there are ... top-brand carrying cases to take the little canine or feline darlings ... compelled to buy some pricey toys at the pet store. But ...
(Date:1/14/2014)... (PRWEB) January 14, 2014 In recent ... motives and methods in product development and promotion has ... industry. This mistrust, fueled by concerns about the insidious ... by reports of spectacular fines to the world’s biggest ...
Breaking Biology Technology:Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3
(Date:4/23/2014)... diner ripping open a dinner roll, a fuel cell ... a hydrogen molecule. Now researchers have captured a view ... of its hydrogen feast. The view confirms previous hypotheses ... work better for alternative energy uses. , This study ... the hydrogen halves end up in the structure of ...
(Date:4/23/2014)... the source of a unique rhythmic sound, recorded for ... is the Antarctic minke whale ( Balaenoptera bonaerensis ). ... 1960s who thought it sounded like a duck, the ... the Southern Ocean, but its source has remained a ... team of researchers deployed acoustic tags on two Antarctic ...
(Date:4/23/2014)... Buenos Aires yesterday, Argentina joins the European Molecular ... The move strengthens the ties between the European ... Argentinian scientists access to EMBL,s world-class facilities and ... since its inception, fostering excellent life science research ... core of EMBL,s mission," says EMBL Director General ...
Breaking Biology News(10 mins):Halving hydrogen 2Halving hydrogen 3Halving hydrogen 4Scientists identify source of mysterious sound in the Southern Ocean 2Scientists identify source of mysterious sound in the Southern Ocean 3
... Species of mammals and birds are threatened with extinction as ... McKee and colleagues from The Ohio State University in the ... the exponential growth of the human population will continue to ... does not appear to be a threshold above which population ...
... at the University of Pittsburgh has developed antibacterial compounds, ... be potential treatments for drug-resistant bacterial infections and appear ... small, making them inexpensive and easy to manufacture. The ... the journal Antimicrobial Agents and Chemotherapy . ...
... MINNETONKA, Minn. , June 19, 2013 MedNet ... clinical study management systems, is pleased to announce its participation ... 23-27 in Boston .  This represents the ... cloud-based eClinical solutions, has exhibited at this premier industry event. ...
Cached Biology News:Are we pushing animals over the edge? 2HIV-derived antibacterial shows promise against drug-resistant bacteria 2MedNet Solutions To Spotlight iMedNet EDC At The 49th DIA Annual Meeting 2
SphingoStripsTM contain fifteen different biologically active, nitrocellulose-immobilized lipids at 100 pmol per spot, which can be assayed for protein binding and specificity using a simple blot ove...
Mouse monoclonal antibody raised against a partial recombinant KRT4. NCBI Entrez Gene ID = KRT4...
... PrecisION Ion Channel Cell Lines ,High ... ,Ion channels are well known for having ... function and consequently have a key function ... that modulate ion channels have been investigated ...
... Channel Cell Lines ,High Quality, Functionally-Validated, Ion ... well known for having a critical role ... have a key function in pain, CNS ... channels have been investigated in therapeutic areas, ...
Biology Products: