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Keys to Successful Densitometry

Eagle Sight software aids image acquisition and processing

Michelle Cayouette *Michael Steege * Jane Moores
Stratagene Cloning Systems

Stratagenes new Eagle Sight software version 3.0 allows researchers to determine integrated density and concentration information from stained nucleic acids or proteins in gels. These functions provide a tool for quickly evaluating experimental data moments after an image has been acquired. To achieve more precise integrated density and concentration determinations, researchers using Eagle Sight software should understand how pixel saturation, background levels and analysis area can affect the accuracy of densitometry results.

The Eagle Eye II still video system is a digital imaging system that has been designed to document and analyze data from a wide variety of laboratory experiments. The newest version of the systems software, Eagle Sight software version 3.0, contains many features that aid image acquisition and processing.1,2 This version of Eagle Sight software can be used for determining molecular weight, integrated density and concentration information from applications such as stained nucleic acid gels or protein gels. The softwares densitometry functions are designed to provide results quickly and easily, even for researchers who have little experience working with the basic principles of densitometry. However, when the effects of pixel saturation, background levels and analysis area on densitometry calculations are understood, the analysis of integrated density and concentration will become more precise.

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