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Isolation to Functional Analyses

he mirVana miRNA Probe Construction Kit to prepare in vitro transcribed probes. Other reagents useful for small RNA analysis include Decade Markers, a set of small RNA markers for polyacrylamide gel electrophoresis, and ULTRAhyb-Oligo, an ultrasensitive hybridization buffer optimized for Northern analysis using small RNA probes.

Like the study of protein-encoding genes, miRNA expression analysis could potentially identify miRNAs involved in many important processes (see MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers). The early results of miRNA studies suggest that these molecules play key roles in many biological processes, including differentiation and oncogenesis. Functional studies featuring reagents for over- and under-expressing miRNAs will then enable a better understanding of miRNAs just as cDNA expression vectors and siRNAs have done for other genes. For example, synthetic pre-miRNAs (Pre-miR miRNA) and antisense miRNAs (Anti-miR miRNA Inhibitors) have been developed to study gene expression and other phenotypic changes in transfection assays (see Reagents for Up- and Down-Regulation of miRNA Activity in Mammalian Cells). Also, the pSilencer 4.1-CMV series of vectors now enables researchers to conduct long-term ectopic miRNA expression and gene silencing exper
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