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Isolation to Functional Analyses

protein levels. For examples of experimental data, see Isolate Total RNA and Protein From the Same Sample.


Preisolated Small RNA

Depending on the isolation method used, commercially prepared RNA may lack some or all of the small RNA fraction. To address this problem, Ambion scientists have optimized isolation procedures used to generate FirstChoice Total RNA from normal human, mouse, and rat tissues, and certify these RNAs to contain small RNAs including miRNA, siRNA, and small nuclear RNA.


Small RNA Analyses

Ambion has developed a series of kits to simplify the detection of small RNAs by solution hybridization, Northern analysis, or in situ hybridization. The mirVana miRNA Detection Kit uses a sensitive solution hybridization assay to detect small RNA species in total RNA populations. Solution hybridization allows detection of multiple miRNAs as well as siRNAs and their mRNA targets in the same assay (for examples of experimental data generated with this kit, see Detecting Attomole Amounts of Small RNA [12]. To simplify probe synthesis for small RNA analysis, Ambion has also introduced the mirVana Probe & Marker Kit to prepare 5' end labeled probes and t
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