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Isolation to Functional Analyses

e recovery of RNA species smaller than ~200 nt. Likewise, current RT-PCR methods developed for mRNA quantitation are incompatible with miRNA and siRNA analyses because these small RNAs are only about the size of the primers used for reverse transcription.

Ambion has developed methods specifically designed for small RNA isolation and quantitation to facilitate miRNA expression studies. These methods are also useful for the quantitation of siRNAs expressed by or introduced into cells and tissues. The mirVana miRNA Isolation Kit uses a rapid, modified GFF-based procedure to recover all RNA, including small RNA species, from cultured cell and tissue samples. With this kit, RNA samples can also be specifically enriched for RNAs smaller than ~200 nt. Such enrichment can dramatically increase the sensitivity of downstream assays. For examples of experimental data, see New Tools for Small RNA Analysis.


Obtaining Samples Containing miRNAs, siRNAs, and Other Small RNAs

The new mirVana PARIS Kit goes one step further. With this kit you can isolate protein and small RNA-containing total RNA from the same sample. The mirVana PARIS procedure also includes a protocol to enrich the population of small RNAs to increase sensitivity of downstream experiments. These features make this kit ideal for correlating mRNA, miRNA, or siRNA levels with
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