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Isolation to Functional Analyses


While the microRNA (miRNA) field is rapidly expanding, these small, non-poly(A) tailed molecules still pose challenges for research. Most RNA analysis techniques have been optimized for longer molecules, not small 21-23 nt RNAs. Their small size precludes standard RT-PCR analysis, and purification and amplification methods that make use of a poly(A) tail, present on mRNAs, won't work.

Here and inside this issue we describe research tools developed specifically for miRNA analysis, including isolation and enrichment of the small RNA fraction, internal high specific activity labeling as well as end labeling of miRNA molecules, up and down modulation of expression, quantitative detection, and profiling via array analysis.

MicroRNAs (miRNAs) are an important class of small RNA molecules that are expressed in eukaryotes. Although the first miRNA was identified in a genetic screen in 1993 [1], it was not until 2001 that the breadth of the miRNA gene class was recognized with the cloning and sequencing of more than one hundred miRNAs from worms, humans, and mice [1-4]. These evolutionarily conserved, noncoding RNA molecules regulate translation of mRNAs through base pair interactions [reviewed in 5]. With some exceptions, worm and human miRNAs inhibit translation of specific mRNAs, while plant miRNAs induce mRNA degradation.

In cells, the ~22 nt mature, functional miRNAs are produced by a recently descr
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Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
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