Navigation Links
Isolation of Microorganisms

>Isolation was carried out with special sterile glass capillaries. The dimensions of these microcapillaries depend on the size of the cells to be isolated. For typical experiments, capillaries with an inner diameter of 5 m and a tip angle of 45 were used. These capillaries were designed in accordance with our specifications (Eppendorf).

Dried bacteria cultures were resuspended using suitable sterile nutrient solutions. The isolated cells were then transferred into the appropriate micro test tubes for further processing.

Advantages
  • This process can be used for cloning and isolating microorganisms (such as bacteria, archaea, protozoa and fungi) as well as eukaryotic organelles and cell nuclei.
  • The risk of contamination during the identification of non-cultivatable microorganisms from mixed cultures by means of single cell PCR is greatly reduced.
  • Microorganisms present in low numbers can also be examined.
  • In addition to aerobic microorganisms, it is also possible to isolate anaerobic microorganisms (with the appropriate accessories).
  • The electronic control of the micro-manipulator and the high-resolution piston pump guarantee rapid and simple isolation.
Conclusion Microorganisms which were previously difficult to access can now be isolated and characterized rapidly using this simple and relatively inexpensive method.

J. Frhlich and H. Knig (1999) Rapid isolation of single microbial cells from mixed natural and laboratory populations with the aid of a micromanipulator. System. Appl. Microbiol. 22, 249-257
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Basic Plasmid DNA Isolation Protocol
10. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
11. RNA-free Plasmid DNA Isolation Protocol
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Isolation Microorganisms

(Date:1/22/2015)... DIEGO , Jan. 22, 2015   Cypher ... and Sequenom, Inc. (NASDAQ: SQNM ), ... development agreement for next generation noninvasive prenatal tests ... genome interpretation technology, called Mantis™, to advance analysis ...
(Date:1/22/2015)... , Jan. 22, 2015   GenoSpace , a precision ... tools to enable the broad use of genomic, imaging and ... the appointment of Michelle Munson , CEO of Aspera, ... Logo - http://photos.prnewswire.com/prnh/20150122/170713 ...
(Date:12/25/2014)... The report “Service Quality Management and Telco Customer Experience ... Global Advancements, Worldwide Forecasts & Analysis (2014-2019)” defines and ... of revenues. , Browse 152 market data tables, ... on “Service Quality Management (SQM) and Telco Customer Experience ...
(Date:12/24/2014)... NEW YORK , Dec. 24, 2014 PlasmaTech ... care in critical areas, announced the closing of an underwritten ... to purchase up to an aggregate 3,500,000 shares of common ... $.01 per warrant.  The warrants have a per share exercise ...
Breaking Biology Technology:Cypher Genomics and Sequenom Announce Development Agreement 2Cypher Genomics and Sequenom Announce Development Agreement 3Cypher Genomics and Sequenom Announce Development Agreement 4GenoSpace Expands Board with Appointment of Michelle Munson 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 3Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 4PlasmaTech Biopharmaceuticals, Inc. Announces Closing of Public Offering 2PlasmaTech Biopharmaceuticals, Inc. Announces Closing of Public Offering 3
... Calif., June 2 BioGenex, a developer of innovative ... for molecular diagnostics, announced today that Abbott has entered ... and distribution agreement to market the Xmatrx(R) platform for ... the terms of the agreement Abbott customers will have ...
... ... MRI reagent, Cell Sense. , ... Pittsburgh, PA (Vocus) June 2, 2009 -- Celsense, Inc. announced today that it is one ... The funded studies will be the first effort to evaluate Cell Sense, the Company’s fluorocarbon-based ...
... VEENENDAAL, The Netherlands, June ... company, today announced the creation,of an exclusive partnership ... BV for an undisclosed sum. The two companies, ... and the combination,will strengthen Nucletron,s position as global ...
Cached Biology Technology:Abbott & BioGenex Extend the Technology Co-development and Distribution Agreement for Marketing the Xmatrx(R) Advanced Staining Platform for the Automation of FISH, IHC and ISH Molecular Diagnostic Assays 2Celsense, Inc Receives NIH Grant to Translate Cell Imaging Technology for Clinical Use 2Celsense, Inc Receives NIH Grant to Translate Cell Imaging Technology for Clinical Use 3Dutch Medical Equipment Producer Nucletron Strengthens Leading Position in Cancer Radiation Therapy Market 2Dutch Medical Equipment Producer Nucletron Strengthens Leading Position in Cancer Radiation Therapy Market 3
(Date:12/17/2014)... Dec. 15, 2014 The Defense Logistics Agency is ... and prevent counterfeit microcircuits from entering into its supply ... in-house microcircuit anti-counterfeit initiative dubbed DNA marking. The capability ... their reliability throughout the supply chain. The new quality ...
(Date:12/15/2014)... DUBLIN , Dec. 12, 2014 Research and Markets ... of the "Global Facial Recognition Market 2015-2019" ... http://photos.prnewswire.com/prnh/20130307/600769 Facial recognition is a technology ... measures the overall facial feature of a person such ...
(Date:12/10/2014)... 8, 2014 You,ve been here before: you desperately need to ... site key or the answer to your secret question. What,s your ... Today, Hoyos Labs , a digital infrastructure security company, launches ... the frustration that comes with usernames, passwords and PINs – ...
Breaking Biology News(10 mins):Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 2Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 3Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3
... This international collaboration has been the largest ever to ... genetic differences between patients infected by HIV, and is ... of infectious disease. Catalan participants have been coordinated by ... of Hospital Germans Trias i Pujol, and Josep M. ...
... how does the cell know to send the signal that ... good sensations, the answer appears to be sugar. , ... in Nature Neuroscience online proposes a role for sugar as ... and brings news of nicotine’s arrival. , Structural biologist ...
... The length of the day relative to night, or ... flowering in many plant species. Short day (SD) plants require ... in order to flower. These are plants that flower as ... temperate regions. Long day (LD) plants will flower when nights ...
Cached Biology News:Identified main genetic variants involved in response to HIV 2Nicotine rush hinges on sugar in neurons 2Research identifies protein that signals flowering in squash plants 2Research identifies protein that signals flowering in squash plants 3
The VersArray hybridization chamber is used for manual (coverslip) hybridization of microarrays. The chamber accommodates 2 slides and is watertight to allow incubation of slides in a water bath. Dim...
5X StabilCoat Immunoassay Stabilizer is a 5X concentrate solution of StabilCoat Immunoassay Stabilizer. The shelf-life of 5X StabilCoat Immunoassay Stabilizer is 2 years when stored at 4C. Product ...
... Yellow crystalline solid. PROTECT FROM LIGHT. Useful ... not required. Purity: ≥98% by TLC. Soluble ... systems, preparation of a 10 mM stock in DMSO ... added to H 2 O with rapid mixing (e.g. ...
Monoclonal antibodies conjugated with rhodamine...
Biology Products: