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Isolation of Microorganisms

>Isolation was carried out with special sterile glass capillaries. The dimensions of these microcapillaries depend on the size of the cells to be isolated. For typical experiments, capillaries with an inner diameter of 5 m and a tip angle of 45 were used. These capillaries were designed in accordance with our specifications (Eppendorf).

Dried bacteria cultures were resuspended using suitable sterile nutrient solutions. The isolated cells were then transferred into the appropriate micro test tubes for further processing.

Advantages
  • This process can be used for cloning and isolating microorganisms (such as bacteria, archaea, protozoa and fungi) as well as eukaryotic organelles and cell nuclei.
  • The risk of contamination during the identification of non-cultivatable microorganisms from mixed cultures by means of single cell PCR is greatly reduced.
  • Microorganisms present in low numbers can also be examined.
  • In addition to aerobic microorganisms, it is also possible to isolate anaerobic microorganisms (with the appropriate accessories).
  • The electronic control of the micro-manipulator and the high-resolution piston pump guarantee rapid and simple isolation.
Conclusion Microorganisms which were previously difficult to access can now be isolated and characterized rapidly using this simple and relatively inexpensive method.

J. Frhlich and H. Knig (1999) Rapid isolation of single microbial cells from mixed natural and laboratory populations with the aid of a micromanipulator. System. Appl. Microbiol. 22, 249-257
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