Jrgen Frhlich, Helmut Knig and Dietmar Kahle
Jrgen Frhlich and Helmut Knig; Institute for Microbiology
and Wine Research,
Johannes-Gutenberg-University; Mainz, Germany
Dietmar Kahle, Eppendorf AG
The isolation of microorganisms (such as bacteria or yeast) from complex
mixed cultures and their cultivation in a pure culture is an essential prerequisite
for their precise identification and characterization.
Several different processes are used for this purpose, including the
classic platecasting process invented by Robert Koch, as well as dilution
methods or selective enrichment.
However, it can prove to be very difficult to isolate microbes when the
conditions for their growth are not known or when the microbes grow in
a heterogeneous mixed culture in very small numbers only.
Such experiments are now possible thanks to new techniques which have
been developed recently (e.g., optical laser tweezers).
This article outlines a novel method which, with the aid of a micromanipulation
workstation, enables the isolation of individual bacteria from a mixed
These cells can then be cultivated further or analyzed via single cell
Isolation of individual bacteria
The workstation consists of an inverse research microscope with up to
100-fold magnification (phase contrast), an Eppendorf Micromanipulator 5171
device as well as the Eppendorf CellTram
Oil hydraulic piston pump.
Source:Page: All 1 2 3 Related biology technology :1
. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood2
. High-Throughput Isolation of Total RNA3
. Simple Isolation of RNA from Tissue and Cultured Cells4
. Fast Isolation of Total RNA from Small Numbers of Cells5
. Comparison of Growth Techniques and Media for the Purpose of Plasmid
Isolation from E. coli Using the Eppendorf
Perfectprep Plasmid Mini
. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini
. Mouse Tail Genomic DNA Isolation Protocol(1)8
. Genomic DNA Isolation Protocol(1,2,3)9
. Basic Plasmid DNA Isolation Protocol10
. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock
. RNA-free Plasmid DNA Isolation Protocol