Navigation Links
Isolation of Microorganisms

Jrgen Frhlich, Helmut Knig and Dietmar Kahle
Jrgen Frhlich and Helmut Knig; Institute for Microbiology and Wine Research,
Johannes-Gutenberg-University; Mainz, Germany
Dietmar Kahle, Eppendorf AG Problem The isolation of microorganisms (such as bacteria or yeast) from complex mixed cultures and their cultivation in a pure culture is an essential prerequisite for their precise identification and characterization.

Several different processes are used for this purpose, including the classic platecasting process invented by Robert Koch, as well as dilution methods or selective enrichment.

However, it can prove to be very difficult to isolate microbes when the conditions for their growth are not known or when the microbes grow in a heterogeneous mixed culture in very small numbers only.

Such experiments are now possible thanks to new techniques which have been developed recently (e.g., optical laser tweezers).

This article outlines a novel method which, with the aid of a micromanipulation workstation, enables the isolation of individual bacteria from a mixed culture.

These cells can then be cultivated further or analyzed via single cell PCR*.

Isolation of individual bacteria Material The workstation consists of an inverse research microscope with up to 100-fold magnification (phase contrast), an Eppendorf Micromanipulator 5171 or TransferMan device as well as the Eppendorf CellTram Oil hydraulic piston pump.

Isolation was carried out with special sterile glass capillaries. The dimensions of these microcapillaries depend on the size of the cells to be isolated. For typical experiments, capillaries with an inner diameter of 5 m and a tip angle of 45 were used. These capillaries were designed in accordance with our specifications (Eppendorf).

Dried bacteria cultures were resuspended using suitable sterile nutrient solutions. The isolated cells were then transferred into the appropriate micro test tubes for further processing.

Advantages
  • This process can be used for cloning and isolating microorganisms (such as bacteria, archaea, protozoa and fungi) as well as eukaryotic organelles and cell nuclei.
  • The risk of contamination during the identification of non-cultivatable microorganisms from mixed cultures by means of single cell PCR is greatly reduced.
  • Microorganisms present in low numbers can also be examined.
  • In addition to aerobic microorganisms, it is also possible to isolate anaerobic microorganisms (with the appropriate accessories).
  • The electronic control of the micro-manipulator and the high-resolution piston pump guarantee rapid and simple isolation.
Conclusion Microorganisms which were previously difficult to access can now be isolated and characterized rapidly using this simple and relatively inexpensive method.

J. Frhlich and H. Knig (1999) Rapid isolation of single microbial cells from mixed natural and laboratory populations with the aid of a micromanipulator. System. Appl. Microbiol. 22, 249-257 .


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Basic Plasmid DNA Isolation Protocol
10. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
11. RNA-free Plasmid DNA Isolation Protocol
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... June 23, 2016 Houston Methodist Willowbrook ... Cy-Fair Sports Association to serve as their official ... Houston Methodist Willowbrook will provide sponsorship support, athletic ... with association coaches, volunteers, athletes and families. ... Cy-Fair Sports Association and to bring Houston Methodist ...
(Date:6/23/2016)... YORK , June, 23, 2016  The Biodesign ... to envision new ways to harness living systems and ... Modern Art (MoMA) in New York City ... than 130 participating students, showcased projects at MoMA,s Celeste ... Paola Antonelli , MoMA,s senior curator of architecture ...
(Date:6/23/2016)... ... June 23, 2016 , ... STACS DNA Inc., the sample tracking software ... State Crime Laboratory, has joined STACS DNA as a Field Application Specialist. , ... Tremblay, President and COO of STACS DNA. “In further expanding our capacity as a ...
(Date:6/23/2016)...  Blueprint Bio, a company dedicated to identifying, protecting ... has closed its Series A funding round, according to ... "We have received a commitment from Forentis Fund that ... meet our current goals," stated Matthew Nunez . ... complete validation on the current projects in our pipeline, ...
Breaking Biology Technology:
(Date:3/31/2016)... 31, 2016   ... the "Company") LegacyXChange is excited to release ... soon to be launched online site for trading 100% ... ) will also provide potential shareholders a sense of ... to an industry that is notorious for fraud. The ...
(Date:3/29/2016)... 29, 2016 LegacyXChange, Inc. (OTC: ... and SelectaDNA/CSI Protect are pleased to announce our successful ... a variety of writing instruments, ensuring athletes signatures against ... collectibles from athletes on LegacyXChange will be assured of ... DNA. Bill Bollander , CEO states, ...
(Date:3/22/2016)... March 22, 2016 ... Sensors Market for Consumer Industry by Type (Image, ... Application (Communication & IT, Entertainment, Home Appliances, ... Forecast to 2022", published by MarketsandMarkets, the ... to reach USD 26.76 Billion by 2022, ...
Breaking Biology News(10 mins):