Navigation Links
Isolation of Microorganisms

Jrgen Frhlich, Helmut Knig and Dietmar Kahle
Jrgen Frhlich and Helmut Knig; Institute for Microbiology and Wine Research,
Johannes-Gutenberg-University; Mainz, Germany
Dietmar Kahle, Eppendorf AG Problem The isolation of microorganisms (such as bacteria or yeast) from complex mixed cultures and their cultivation in a pure culture is an essential prerequisite for their precise identification and characterization.

Several different processes are used for this purpose, including the classic platecasting process invented by Robert Koch, as well as dilution methods or selective enrichment.

However, it can prove to be very difficult to isolate microbes when the conditions for their growth are not known or when the microbes grow in a heterogeneous mixed culture in very small numbers only.

Such experiments are now possible thanks to new techniques which have been developed recently (e.g., optical laser tweezers).

This article outlines a novel method which, with the aid of a micromanipulation workstation, enables the isolation of individual bacteria from a mixed culture.

These cells can then be cultivated further or analyzed via single cell PCR*.

Isolation of individual bacteria Material The workstation consists of an inverse research microscope with up to 100-fold magnification (phase contrast), an Eppendorf Micromanipulator 5171 or TransferMan device as well as the Eppendorf CellTram Oil hydraulic piston pump.

Isolation was carried out with special sterile glass capillaries. The dimensions of these microcapillaries depend on the size of the cells to be isolated. For typical experiments, capillaries with an inner diameter of 5 m and a tip angle of 45 were used. These capillaries were designed in accordance with our specifications (Eppendorf).

Dried bacteria cultures were resuspended using suitable sterile nutrient solutions. The isolated cells were then transferred into the appropriate micro test tubes for further processing.

Advantages
  • This process can be used for cloning and isolating microorganisms (such as bacteria, archaea, protozoa and fungi) as well as eukaryotic organelles and cell nuclei.
  • The risk of contamination during the identification of non-cultivatable microorganisms from mixed cultures by means of single cell PCR is greatly reduced.
  • Microorganisms present in low numbers can also be examined.
  • In addition to aerobic microorganisms, it is also possible to isolate anaerobic microorganisms (with the appropriate accessories).
  • The electronic control of the micro-manipulator and the high-resolution piston pump guarantee rapid and simple isolation.
Conclusion Microorganisms which were previously difficult to access can now be isolated and characterized rapidly using this simple and relatively inexpensive method.

J. Frhlich and H. Knig (1999) Rapid isolation of single microbial cells from mixed natural and laboratory populations with the aid of a micromanipulator. System. Appl. Microbiol. 22, 249-257.


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Basic Plasmid DNA Isolation Protocol
10. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
11. RNA-free Plasmid DNA Isolation Protocol
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Isolation Microorganisms

(Date:7/29/2014)... SoundConnect , an award winning ... to announce the strategic additions of implementation, support, ... Charleston and Atlanta offices. SoundConnect’s organizational expansion ... hosted solutions, collaboration and mobility that improve business ... SoundConnect was founded in 2004, with the mission ...
(Date:7/29/2014)... (PRWEB) July 29, 2014 The advanced ... the brain that are driving a revolution in the ... new peer-reviewed journal published by SPIE, the international ... issue coincides with the launch of the BRAIN Initiative ... articles that lay out a vision for and provide ...
(Date:7/29/2014)... The Quantum Cheshire Cat: Can a particle be separated ... Nature Communications , published the results of the ... magnetic field, conducted by Chapman University in Orange, CA, ... often seen a cat without a grin," thought Alice ... the most curious thing I ever saw in my ...
(Date:7/29/2014)... , July 29, 2014  The Society ... new and updated program for the 9 th ... 12, 2014 in Amelia Island , Florida.  ... and investigators remains unchanged, however, the topics and ... and networking opportunity among all stakeholders with a ...
Breaking Biology Technology:SoundConnect Organization and Culture is Evolving 2SPIE Launches 'Neurophotonics' Journal with Special Section on BRAIN Initiative 2SPIE Launches 'Neurophotonics' Journal with Special Section on BRAIN Initiative 3SPIE Launches 'Neurophotonics' Journal with Special Section on BRAIN Initiative 4The quantum Cheshire cat: Scientists separate a particle from its properties 2The quantum Cheshire cat: Scientists separate a particle from its properties 3The quantum Cheshire cat: Scientists separate a particle from its properties 4TransCelerate, Lilly, Pfizer, and FDA Highlight Collaborative Program at the Site Solutions Summit 2TransCelerate, Lilly, Pfizer, and FDA Highlight Collaborative Program at the Site Solutions Summit 3
... MADISON -- The same chemical reaction that causes iron to ... stress chips away at healthy cells and is a process, ... conditions in humans ranging from Alzheimer,s, heart disease and stroke ... writing in the current edition (Feb. 21, 2008) of the ...
... N.C., Feb. 20 Addrenex,Pharmaceuticals has reached its ... a new drug application (NDA) for its first ... Food and Drug,Administration, Addrenex and its licensing partner ... first half of 2009., Addrenex has moved ...
... SGI Altix, Altix XE Cluster and SGI InfiniteStorage ... Environment for All Stages of Research, SUNNYVALE, ... Annual Lorne Genome Conference -- To create an ... bioinformatics,research, the Malaysia Genomics Institute (MGI) selected SGI ...
Cached Biology Technology:Genetic pathway critical to disease, aging found 2Genetic pathway critical to disease, aging found 3Addrenex Pharmaceuticals Reaches Milestone with Filing of NDA for First Product, CloniBID, to Treat Hypertension 2Malaysia Genome Institute Advances Bioinformatics Research Using SGI Technology 2Malaysia Genome Institute Advances Bioinformatics Research Using SGI Technology 3Malaysia Genome Institute Advances Bioinformatics Research Using SGI Technology 4
(Date:7/30/2014)... Washington, DCToday scientists unveiled the first high-resolution map ... the entire country of Per. The new and ... a sea change for future market-based carbon economies. ... extremely high ecological diversity and it provides the ... degradation for conservation, land use, and enforcement purposes. ...
(Date:7/29/2014)... in the University of Bristol,s Nutrition and Behaviour Unit ... into account when planning meals. , The research, to ... Ingestive Behavior Conference (SSIB 2014) in Seattle, USA this ... do. , The team was led by Professor Jeff ... Psychology. , As part of a Biotechnology and Biological ...
(Date:7/29/2014)... warms and sea ice retreats, the North is changing. ... open water which is predicted to extend across the ... Storms thus have the potential to create Arctic swell ... unpredictable element to the region. , A University of Washington ... middle of the Arctic Ocean, and detected house-sized waves ...
Breaking Biology News(10 mins):Peru's carbon quantified: Economic and conservation boon 2Peru's carbon quantified: Economic and conservation boon 3Huge waves measured for first time in Arctic Ocean 2Huge waves measured for first time in Arctic Ocean 3
... GEOLOGY ends 2012 with 23 new articles spanning ... Naica Cave, Mexico; Diamantina, Brazil; the Galpagos hotspot; China; ... central West Greenland; the California arc; the Pacific Ring ... Sierra Nevada; Spain; New Zealand; Turkey; Connecticut, USA; and ...
... the extinct giant piranha, or megapiranha, have the most ... body size is taken into account, finds researchers in ... The research paper, Mega-Bites: Extreme jaw forces of living ... which allows them to attack and bite chunks out ...
... RICHLAND, Wash. Pacific Northwest National Laboratory has been ... large amounts of renewable energy until it,s needed, fight ... to the marketplace. The Federal Laboratory Consortium announced ... Richland is receiving three 2013 Excellence in Technology Transfer ...
Cached Biology News:GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 2GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 3GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 4GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 5GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 6GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 7GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 8GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 9GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 10GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 11GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 12GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 13GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 14GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 15GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 16GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 17GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 18GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 19GEOLOGY returns to Naica Cave, Mexico, and extends its reach to Mercury 20Black piranha, megapiranha have most powerful bites of fish living or extinct, finds GW researcher 2PNNL recognized for transferring innovations to the marketplace 2PNNL recognized for transferring innovations to the marketplace 3
... commonly used technique for studying protein function ... on SDS-PAGE and transferred to a membrane ... are probed with specific antibodies. Unlike nucleic ... Southern and Northern blots, it has been ...
... System, Basic Package Superior Protein Detection ... The ProXPRESS 2D Proteomic Imaging System is ... both proteomic and general life science research ... the use of all dyes in the ...
... our Regulatory Edition of Molecular Imaging (MI) ... for life science research and drug discovery ... with U.S. Food and Drug Administrations Code ... Electronic Records; Electronic Signatures [21 CFR Part ...
... OPA1-01009, OPEP-01009 is a 16 amino acid (amino acids 3-18) ... (DFF). The sequence of this peptide is (amino to ... S - V - K - L - R - ... R - K(18). This peptide may be used ...
Biology Products: