Navigation Links
Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A

on techniques for recovering the proteins from gels are not needed. Finally, microsequencing can be performed directly from these solutions since tricine, unlike glycine, does not interfere with Edman degradation.


Acknowledgements

This work was supported by National Institutes of Health Grant HL-24530, NATO Grant CRG-940595, The Blowitz Foundation, and the American Heart Association Student Scholar in Cardiovascular Disease and Stroke Award. This work was conducted under the auspices of the Association for United States-French Biomedical Cooperation.


References
1. Moncada, S. and Vane, J. R., Pharmacol. Rev., 30, 293331 (1978).

2. Kurtenbach, E., Curtis, C. A. M., Pedder, E. K., Aitken, A., Harris, A. C. M. and Hulme, E. C., J. Biol. Chem., 265, 1370213708 (1990).

3. Dohlman, H. G., Thorner, J., Caron, M. G. and Lefkowitz, R. J., Annu. Rev. Biochem., 60, 653688 (1991).

4. Cohen, J. B., Sharp, S. D. and Liu, W. S., J. Biol. Chem., 266, 2335423364 (1991).

5. Boyd, N. D., Kage, R., Dumas, J. J., Krause, J. E. and Leeman, S. E., Proc. Natl. Acad. Sci., 93, 433437 (1996).

6. Servant, G., Laporte, S. A., Leduc, R., Escher, E. and Guillemette, G., J. Biol. Chem., 272, 86538659 (1997).

7. Schagger, H. and von Jagow, G., Anal. Biochem., 166, 368379 (1987).

8. Laemmli, U. K., Nature (London), 227, 680-685 (1970).

9. Gross, E., Methods in Enzymology, 11, 238-253 (1967).


Coomassie is a trademark of ICI


back to top
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
(Date:7/2/2008)...y 2 /- Bioheart, Inc. (Nasd...on the development of,autologous (patient-derived)...hronic and acute heart damage, today announced it ... the Russell Investment Group reconstituted its,co...on June 27, 2008., According to the Russell Inves...
(Date:7/2/2008)...f., July 2 /- Pearl Therapeutics, Inc... for major respiratory,diseases, today announced t... round of financing. This financing was executed a...ch closed in May 2007. Participating in the,intern...ntures, New Leaf,Ventures, 5AM Ventures and Nektar...
(Date:7/2/2008)...uly 2 /Xinhua-- WuXi PharmaTech (NYSE... medical device research,and development outsourci...,States, today announced that it has appointed Mr....Logo: http://www.newscom.com/cgi-bin/prnh/2004070..., Mr. Zhu worked as Head of Asia Human,Resources w...
(Date:7/1/2008)...uly 1 /- Microfluidic Systems Inc.,(M...Phase 3X agreement for,the production of Microflui...ND). This award is the fourth in a series of funde...rate since 2003. The continued funding,through Mar...utomated,systems for extended field trials in key ...
Breaking Biology Technology:Bioheart, Inc., Added to Russell Microcap Index 2Pearl Therapeutics Closes $18 Million Financing Round Led By Committed Investors 2WuXi PharmaTech (NYSE: WX) Appoints Mr. Bob Zhu as VP of HR 2Microfluidic Systems Receives Continued Funding From the Department of Homeland Security (DHS) Toward the Commercial Production of Autonomous Airborne Pathogen Detection Systems 2Microfluidic Systems Receives Continued Funding From the Department of Homeland Security (DHS) Toward the Commercial Production of Autonomous Airborne Pathogen Detection Systems 3
...AY, Wis. The war against computer viruses and spa... a software products and services company based in...us announced last week the availability of a new ...ndry Network Appliance , which is powered by its M...o human intervention the new product can catch 99 ...
...eone or something from the U.S.A. But the term is ...ets. Signs in D.C. announce American University. ...linds. Given that there are millions of trademarks... to be used as a trademark by a variety of busines... keyword in todays e-commerce market and is at the...
...ress MADISON, Wis. Members of the World Future S...n emerging trend in the corporate world the use o...an, owner of the consulting firm Partners for Prog...ce book Critical Shift, explained how simply follo...s, creating better communication, recall and commi...
...he rude noise somebody makes in a meeting after to...ulation also knows that Enterprise Resource Planni...de functional problems with a single check to a ve... today. What is apparently less obvious is that ER... into several situations recently where portfolio-...
Other Biology Technology:Green Bay entrepreneur launches new message filtering solution 2Green Bay entrepreneur launches new message filtering solution 3Thats my trademark 2Thats my trademark 3Thats my trademark 4Storytelling makes a comeback in an unlikely place 2Storytelling makes a comeback in an unlikely place 3Steering a project portfolio takes more than expensive software 2Steering a project portfolio takes more than expensive software 3
(Date:7/3/2008)... , ST. LOUIS Want to slow the signs of aging and...suggests cutting back on calories could be a promi...een shown to slow the aging process in rats and mi...iction affects the aging process in rodents, one p...sing a thyroid hormone, triiodothyronine (T3), whi...
(Date:7/2/2008)... , Arlington, Virginia (July 2, 2008) Led by a b...s industrial powers have the opportunity at the up...bstantive action against climate change. , Germa...million Euros over four years to conserve tropical... forest protection, starts to address a major sour...
(Date:7/2/2008)... , COLLEGE STATION, July 2, 2008 -- Two Texas A&M...onal tool that will help scientists more accuratel...perons, in bacteria. The tool, which allows scient... more accurate than previous methods because it st... from statistical predictions, they say. The resea...
(Date:7/2/2008)... , Grand Forks, North Dakota, July 2, 2008 Volun...Foundation International (JDRF), the American Diab...lth Board (NIHB) will present Senator Byron Dorgan...his leadership on extending the Special Diabetes P...d at 11:30 a.m. in the Buchli Room at the Hilton G...
Breaking Biology News(10 mins):Looking for the Founatain of Youth? Cut your calories, research suggests 2G8 summit: Opportunity for immediate action on climate change 2G8 summit: Opportunity for immediate action on climate change 3Texas A&M researchers develop tool to study complex clusters of genes 2Texas A&M researchers develop tool to study complex clusters of genes 3Sen. Byron Dorgan to be honored 2Independent Review Organizations Poised to Help Reduce Rising Healthcare Cost 10852 1Independent Review Organizations Poised to Help Reduce Rising Healthcare Cost 10852 2iCAD Ranks 231 in Mammography CAD User Satisfaction 10849 1iCAD Ranks 231 in Mammography CAD User Satisfaction 10849 2iCAD Ranks 231 in Mammography CAD User Satisfaction 10849 3Cell Genesys to Present at the BIO CEO and Investor Conference 2968 1Cell Genesys to Present at the BIO CEO and Investor Conference 2968 2Many Babies Exposed to Chemicals 10844 1Many Babies Exposed to Chemicals 10844 2Many Babies Exposed to Chemicals 10844 3
...s mating systems, reproductive success reflects ph...typic variation can include that of body size and ...nd performance. , In a study published in the Sept...her Lappin (Northern Arizona University) and Jerry...ern collared lizard (Crotaphytus collaris ), a sex...
...aveling the complex mechanisms in the brain that r...petites are controlled by systems very similar to ... type of neuron that is essential for feeding beha...g and undergo rapid weight loss. , Remarkably, t... only influenced eating behavior when they were re...
...ides insight into genetic basis of human diseaseHi...elds a map of genetic variation which can give clu...The current technologies collect genotypes, or inf...et many scientists believe that drilling down to t...l of each chromosome -- so-called haplotypes -- wi...
...the last ice age are the likely culprits in the ex...erican great mammals such as mammoths, mastodons a...ida researcher. , Determining whether the first ar...tinent at the end of the last Ice Age was responsi...ed scientists because both events occurred at the ...
Other Biology News:An essential regulator of body weight revealed 2An essential regulator of body weight revealed 3An essential regulator of body weight revealed 4California computer scientists double volume of data in NIH biotech repository 2California computer scientists double volume of data in NIH biotech repository 3California computer scientists double volume of data in NIH biotech repository 4Study shows big game hunters, not climate change, killed off sloths 2Study shows big game hunters, not climate change, killed off sloths 3
The DNA Engine instrument is the cycler that revolutionized thermal cycling. Unprecedented thermal uniformity and swappable blocks ensure consistent results, experiment-to-experiment and block-to-b
Peroxidase Protective Buffer is a diluent formulated to stabilize the activity of Chemicon horseradish peroxidase (HRP)-antibody conjugates at high conjugate dilution for extended periods.
Clear yellow solution. In PBS containing BSA, 0.01% thimerosal, and other proprietary stabilizers; 0.2 M filtered. Diluent formulated to stabilize activity of horseradish peroxidase antibody conjugat
GIBCO Dulbeccos Modified Eagle Medium (D-MEM); powder (high glucose) from Invitrogen
Biology Products: