Navigation Links
Isolation of Low Molecular Weight Digestion Products of the Human Platelet Thromboxane A2 Receptor, Rev A

Joseph W. Turek and Guy C. Le Breton, Department of Pharmacology, University of Illinois at Chicago, College of Medicine, Chicago, IL 60612. Address correspondence to: Joseph W. Turek, 835 South Wolcott, Department of Pharmacology M/C 868, University of Illinois at Chicago, Chicago, IL 60612. E-mail: jturek1@icarus.uic.edu


Introduction

The human platelet thromboxane A2 (TXA2) receptor, in addition to its normal role in hemostasis, has been implicated in the pathogenesis of multiple cardiovascular diseases.1 Studies suggests that increased platelet production of the natural ligand, TXA2, may lead to enhanced platelet aggregatory activity, predisposing an individual to spontaneous platelet aggregation, followed by thrombus formation. Attempts to develop specific antagonists to block the TXA2 receptor have been hampered by a lack of information concerning the receptor ligand-binding domain.

Labeling of receptors, followed by sequential receptor digestion, accumulation, and sequence identification of labeled fragments, has become a commonly used approach to identify receptor ligand-binding domains.26 However, the initial digestion product contains a wide mixture of proteins, from smaller peptides which cannot be separated by traditional glycine gel electrophoresis, to larger proteins not effectively purified using reversed-phase or size exclusion HPLC. In addition, the limited availability of certain receptor proteins (including the TXA2 receptor) has hindered the isolation of sufficient amounts of labeled fragments. To circumvent these problems, we have coupled continuous elution preparative gel electro
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
(Date:10/1/2014)... Weighing applications in pharmaceutical manufacturing ... product. However, while the pharmaceutical industry is one ... Good Manufacturing Practices (GMP) usually provide only broad ... to interpret these guidelines and assess process risks ... TOLEDO experts have created a new white paper, ...
(Date:10/1/2014)... The participants committed to not only creating unique business ... adjusting, tweaking and testing virtual models and 3D test ... , “I enjoyed being involved as a judge,” stated ... showed a commendable range exploring the technical capabilities of ... and the creativity of the contestants.” , Array Architects ...
(Date:10/1/2014)... (PRWEB) September 30, 2014 Award ... SoundConnect announces four new-hosted collaboration bundles that ... deliver high quality conferencing experiences for low, flat ... to choose from, businesses have optimized solutions to ... solutions include audio only minutes (1K, 5K, ...
(Date:10/1/2014)... 30, 2014 This report analyzes ... US$ Million by the following Product Segments: ... Specific end-use segments also analyzed are Pharmaceuticals ... The report provides separate comprehensive analytics for ... East, and Latin America. Annual estimates and ...
Breaking Biology Technology:Comply with Weighing-Related Regulations in Pharma Manufacturing 2Array Architects Challenges Staff to Create with 3D Printer 2SoundConnect Launches New Audio, Web and Video Bundles 2Global Specialty Enzymes Industry 2Global Specialty Enzymes Industry 3Global Specialty Enzymes Industry 4Global Specialty Enzymes Industry 5Global Specialty Enzymes Industry 6Global Specialty Enzymes Industry 7Global Specialty Enzymes Industry 8Global Specialty Enzymes Industry 9Global Specialty Enzymes Industry 10Global Specialty Enzymes Industry 11Global Specialty Enzymes Industry 12Global Specialty Enzymes Industry 13Global Specialty Enzymes Industry 14Global Specialty Enzymes Industry 15Global Specialty Enzymes Industry 16Global Specialty Enzymes Industry 17Global Specialty Enzymes Industry 18Global Specialty Enzymes Industry 19Global Specialty Enzymes Industry 20Global Specialty Enzymes Industry 21Global Specialty Enzymes Industry 22Global Specialty Enzymes Industry 23Global Specialty Enzymes Industry 24Global Specialty Enzymes Industry 25Global Specialty Enzymes Industry 26
... /PRNewswire-FirstCall/ - Angiotech Pharmaceuticals, Inc. (NASDAQ: ... corporate partner, Boston Scientific Corporation (NYSE: ... Food and Drug Administration (FDA) to market its ... highly deliverable, next-generation drug-eluting stent (DES) specifically designed ...
... Senomyx, Inc. (Nasdaq: SNMX ), a leading ... develop novel flavor ingredients for the food, beverage, and ... L. Linemeyer, Ph.D., to the position of Vice President, ... in directing biology research programs and collaborative research projects ...
... Accumetrics, Inc. announces today it has signed new exclusive ... countries. Distribution of Accumetrics, VerifyNow products will be ... Austria and Switzerland; and Kordia in the Netherlands, Belgium ... life-saving antiplatelet therapies, such as aspirin, Plavix(R) and ...
Cached Biology Technology:Angiotech Pharmaceuticals announces FDA approval of next-generation TAXUS(R) Liberte(R) Atom(TM) Stent system 2Angiotech Pharmaceuticals announces FDA approval of next-generation TAXUS(R) Liberte(R) Atom(TM) Stent system 3Angiotech Pharmaceuticals announces FDA approval of next-generation TAXUS(R) Liberte(R) Atom(TM) Stent system 4Angiotech Pharmaceuticals announces FDA approval of next-generation TAXUS(R) Liberte(R) Atom(TM) Stent system 5SENOMYX ANNOUNCES APPOINTMENT OF DAVID L. LINEMEYER, PH.D., AS VICE PRESIDENT, BIOLOGY 2SENOMYX ANNOUNCES APPOINTMENT OF DAVID L. LINEMEYER, PH.D., AS VICE PRESIDENT, BIOLOGY 3Accumetrics, Inc. Announces European Distribution Channels for France, Austria, Switzerland, Netherlands, Belgium and Luxembourg 2Accumetrics, Inc. Announces European Distribution Channels for France, Austria, Switzerland, Netherlands, Belgium and Luxembourg 3
(Date:9/30/2014)... know what goes wrong with your muscles because of age, ... "normal" actually is. That,s where a new research report published ... Journal comes in. In the report, a team ... that can help scientists "see" which genes are active in ... never-before-detected gene activity and that men have approximately 400 more ...
(Date:9/30/2014)... HEIGHTS, Ill. (September 30, 2014) People who suffer ... can do to control their asthma besides properly taking ... a new article in the Annals of Allergy, ... American College of Allergy, Asthma and Immunology (ACAAI), asthma ... examining their lifestyle. The woman described in the Annals ...
(Date:9/29/2014)... Monday, September 29: Between 1970 and 2010 ... around the globe dropped 52 percent, says the ... Wildlife Fund (WWF). This biodiversity loss occurs disproportionately ... use of high-income countries. , In addition to ... data point to other warning signs about the ...
Breaking Biology News(10 mins):Scientists identify which genes are active in muscles of men and women 2Asthma symptoms kicking up? Check your exposure to air pollution 2Half of global wildlife lost, says new WWF report 2
... The Field Museum announced today that it has received 409,416 ... of Monaco Foundation for a three-year project: Establishing new conservation ... biological inventories by The Field Museum,s Environment, Culture, and Conservation ... landscapes in Ecuador, Peru, and Bolivia. Eight of these areas ...
... CryoLife, Inc. (NYSE: CRY) Elite cardiovascular surgeons from around ... the two-day Ross Summit to review and discuss current peer-reviewed ... and to practice the technical nuances required to perform this ... type of specialized aortic valve surgery in which the patient,s ...
... Rush University Medical Center is participating in a ... to determine whether a vitamin-like substance, in high doses, ... disorder that affects about one million people in the ... we have for Parkinson,s can only mask the symptoms ...
Cached Biology News:Prince Albert II of Monaco Foundation helps fund Field Museum conservation efforts in Peru 2Growing outcomes evidence spurs renewed interest in unique heart valve procedure 2Can an over-the-counter vitamin-like substance slow the progression of Parkinson's disease? 2
Complement C1r [Human]...
... ISO 9001:2000 Certified USDA Research Registered ... SOP's Training AALAS Certified Technicians ... Procedures GLP Documentation Upon Request Focus ... point for all your custom immunology service ...
... ECL Plus Western Blotting Reagent Pack, ... and secondary detection of membrane-bound mouse and ... nitrocellulose or PVDF membranes. Detection reagents are ... for use with ECL Plus. Category: ...
Agar, 5 lb. Bacteriological.Moisture: < 10%, ash: < 6.5%. Category: Nucleotides & Enzymes & Biochemicals, Ultrapure Biochemicals ....
Biology Products: