Navigation Links
Isolation of Cosmids using Eppendorf's Perfectprep Plasmid ,,, Mini Kit

Perfectprep Plasmid Mini Kit to provide good consistent yields per well ranging from 110 g.

Conclusion:

The restriction digest seen in Figure 1 represents the ability of the Eppendorf's Perfectprep Plasmid Mini purification system to purify large construct DNA vectors. The simplicity of the Perfectprep Mini protocol (see Materials and Methods) offers the ability to isolate quality cosmid DNA in high quantities, easily and efficiently. The DNA obtained can be used for several downstream applications other than the one represented here, including transfection and sequencing. Eppendorf's Perfectprep Mini Kit is a simple solution to the diversified needs of researchers involved with DNA vector isolation.

ACKNOWLEDGEMENTS:

I would like to say thank you to James Griffith for his work and results. To Joel Lopez and Vince Prezioso, I would like to say thank you for all your help downstream.

REFERENCES:

  1. Ausubel, F.M., et.al., Current Protocols in Molecular Biology Vol. 1, Wiley and Sons, New York, 2001.
  2. Hartwell, L.H., et.al., Genetic, From Genes to Genomes, McGraw Hill, New York, 2000.
  3. Kelkar, H.S., et.al., The Neurospora Crassa Genome: Cosmid Libraries Sorted by Chromosome, Genetics 157, 2001, 979-990.
  4. New England Bio Labs, Beverly, MA.
  5. Promega Corporation, Madison, WI.

'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Isolation Cosmids using Eppendorf Perfectprep Plasmid Mini Kit

(Date:9/11/2014)... Texas , Sept. 11, 2014 XBiotech ... high throughput filling machine that the Company says will ... The fully integrated system is designed to fill, stopper ... the manufacturing process. Additionally, the unit is said to ... scanning system to provide a high level of quality ...
(Date:9/11/2014)... 11, 2014 The Registration Committee ... is now open for Pittcon 2015, the world’s ... The event will be held March 8-12, 2015, ... Louisiana. , The discounted registration fee of ... after 2/20/2015). This affordable price includes unlimited week-long ...
(Date:9/11/2014)... Francisco, California (PRWEB) September 11, 2014 ... of software and IT systems that support clinical ... Annotation™ (GVA™) Service that now supports the interpretation ... resulting in a single, user-friendly report that contains ... been used toward cancer treatment planning and support ...
(Date:9/11/2014)... 2014 /PRNewswire/ - Portage Biotech Inc. ("Portage" or "the ... that it intends to conduct a Private Placement ... funding its preclinical development activities and financial commitments ... The Company is proposing to complete a ... shares at a price of US$0.10 per Share, ...
Breaking Biology Technology:Conferee Registration Opens for Pittcon 2015; Housing & Travel Open for Reservations 2CollabRx Launches Expansion of Its Genetic Variation Annotation Service to Support Copy Number Variation (CNV) Data 2CollabRx Launches Expansion of Its Genetic Variation Annotation Service to Support Copy Number Variation (CNV) Data 3Portage Announces Proposed Private Placement 2Portage Announces Proposed Private Placement 3Portage Announces Proposed Private Placement 4
... Five exceptional oncology nurses out of nearly 2,400 ... | US Oncology for their dedication to their ... Oncology Nursing Award honors those nurses in the United ... determination in providing the highest quality oncology nursing care to ...
... 22, 2011 The founders of Oncobiologics, Inc. today ... services, will launch itself to the biopharmaceutical industry at ... held June 27-30 at the Walter E. Washington Convention ... by a group of leading scientists and engineers from ...
... features below 100 nanometres have numerous applications ranging ... Tecnalia participates in a intragated FP7 European Project ... on nanoimprinting techniques (NIL), as a core aspect ... benefits followed are cost reduction, simpler fabrication process ...
Cached Biology Technology:McKesson Specialty Care Solutions | US Oncology Honors Five Exceptional Oncology Nurses for Outstanding Work 2McKesson Specialty Care Solutions | US Oncology Honors Five Exceptional Oncology Nurses for Outstanding Work 3McKesson Specialty Care Solutions | US Oncology Honors Five Exceptional Oncology Nurses for Outstanding Work 4Oncobiologics, Inc. to Unveil New Biologics Proof-of-Concept Engine at BIO International Convention 2
(Date:9/12/2014)... Melbourne researchers have discovered that cartilage plays an ... joints seen in rheumatoid arthritis, rather than being ... Tommy Liu, Professor Ian Wicks, Dr Kate Lawler, ... and Eliza Hall Institute made the discovery while ... controlling inflammation during rheumatoid arthritis. The study was ...
(Date:9/12/2014)... Washington , September 12, 2014 ... source covering leading sectors including biometrics and mobile tech, issues ... launch of Apple Pay, its new payment service. ... founder of the Secure Identity and Biometrics Association (SIBA) and ... NXTD ) a company that is introducing its own ...
(Date:9/11/2014)... deadly legacy of DDT, American peregrine falcons ( Falco ... biologists proposed to stop rearing young birds in captivity ... Doug Bell published models that year in ESA,s journal ... for the falcon in California, with and without direct ... that the birds would continue to recover without captive ...
Breaking Biology News(10 mins):No innocent bystander: Cartilage contributes to arthritis 2Biometric Experts Comment on Launch of Apple Pay Smart Wallet Payment Service 2Biometric Experts Comment on Launch of Apple Pay Smart Wallet Payment Service 3Biometric Experts Comment on Launch of Apple Pay Smart Wallet Payment Service 4Biometric Experts Comment on Launch of Apple Pay Smart Wallet Payment Service 5Volunteer 'eyes on the skies' track peregrine falcon recovery in California 2Volunteer 'eyes on the skies' track peregrine falcon recovery in California 3Volunteer 'eyes on the skies' track peregrine falcon recovery in California 4Volunteer 'eyes on the skies' track peregrine falcon recovery in California 5
... in the fruitfly genome, Johns Hopkins scientists have identified those ... captured a global view of all the genes turned on ... to be published April 3 in Developmental Cell, has implications ... move similarly to spread beyond an original tumor, which are ...
... Italy and Virginia Tech in the United States have ... for the production xanthurenic acid (XA) in Anopheles gambiae, ... in the sexual reproduction of the malaria parasite (Plasmodium ... of XA could be an avenue for development of ...
... from a new, five-year study show that regular use of ... cancer by up to 71 percent and may offer similar ... , The study findings were released today at the ... Washington, D.C. , "We believe this is the first ...
Cached Biology News:Researchers identify genes in fruitflies that may shed light on human cancer spread 2Oxidation defense in mosquitoes benefits malaria parasite 2COX-2 inhibitors significantly reduce risk of cancer 2
Anti-Indolamine (Indoleamine) 2,3-dioxygenase Immunogen: Recombinant human IDO. Available Date: 38454...
Anti-Ephrin B1 Host: rabbit polyclonal Species Reactivity: human Applications: WB Storage: 4C...
Human Regulatory T cell Staining Kit #2 (w/ APC Foxp3 PCH101, FITC CD4, PE CD25)...
...
Biology Products: