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Isolation of Cosmids using Eppendorf's Perfectprep Plasmid ,,, Mini Kit

Perfectprep Plasmid Mini Kit to provide good consistent yields per well ranging from 110 g.

Conclusion:

The restriction digest seen in Figure 1 represents the ability of the Eppendorf's Perfectprep Plasmid Mini purification system to purify large construct DNA vectors. The simplicity of the Perfectprep Mini protocol (see Materials and Methods) offers the ability to isolate quality cosmid DNA in high quantities, easily and efficiently. The DNA obtained can be used for several downstream applications other than the one represented here, including transfection and sequencing. Eppendorf's Perfectprep Mini Kit is a simple solution to the diversified needs of researchers involved with DNA vector isolation.

ACKNOWLEDGEMENTS:

I would like to say thank you to James Griffith for his work and results. To Joel Lopez and Vince Prezioso, I would like to say thank you for all your help downstream.

REFERENCES:

  1. Ausubel, F.M., et.al., Current Protocols in Molecular Biology Vol. 1, Wiley and Sons, New York, 2001.
  2. Hartwell, L.H., et.al., Genetic, From Genes to Genomes, McGraw Hill, New York, 2000.
  3. Kelkar, H.S., et.al., The Neurospora Crassa Genome: Cosmid Libraries Sorted by Chromosome, Genetics 157, 2001, 979-990.
  4. New England Bio Labs, Beverly, MA.
  5. Promega Corporation, Madison, WI.

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... fosmid (40kb), and large plasmid insert (10-20kb) ... BAC vector. On command 20-50 fold copy ... DNA purification. Available with blue-white screening (pEZ ... the highest insert stability. Kits include electrocompetent ...
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