Cosmids are commonly used vectors in the lab, providing the capability to hold large fragments of DNA. They often help when creating genomic libraries where small plasmids and large constructs such as BAC's are not appropriate. Purification of cosmids is a necessary step during their use; Eppendorf's Perfectprep Plasmid Mini Kit is a rapid and effective way of purifying cosmid DNA from bacterial culture.
Cosmids have become important tools for molecular biologists, filling
a void left between small plasmid vectors and large vector constructs
such as BAC's. Large vectors such as cosmids are extremely useful in creating
genomic libraries with a capacity to hold fragments of DNA ranging from
30kb and 45kb DNA . Cosmid structures were created from a hybrid of
both phage and plasmid traits . The intrinsic characteristics of cosmids
allow them to undergo efficient ligation, transfection, replication and
selection; providing the exclusivity needed for creating genomic libraries.
The characterization of DNA inserts ligated into cosmids is one of the
most common downstream applications when creating a library. Due to the
size of cosmids it is important to find a method that will purify the
cosmid without shearing or excessive loss of DNA. Eppendorf's Perfectprep
Plasmid Mini Kit is very versatile, providing an