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Isolation and Characterization of Anti-Viral Nucleoside Compounds from the Chinese Herb Taraxacum Mongolicum

Conclusions

This method shows a successful example of using the LC/MS/MS methodology for identifying the presence of nucleotide and nucleoside analogs in crude herbal extract samples. This technology can be used as a general tool to characterize other natural products.

The extraction method was simple and effective. Following a rational study design, the powerful LC/MS/MS method allows a direct injection of a crude herbal extract to characterize active ingredients such as nucleoside and nucleotide analogs in herbs.

The results demonstrated that there may be at least three nucleoside analogs present in the herbal extract of Taraxacum mongolicum. Since this herb has anti-Herpes simplex virus type-1 properties, the nucleoside analogs may be responsible for the anti-viral activities. To positively identify the chemical structures, more experiments are being conducted, such as NMR and Qq-TOF for detailed structure elucidation and exact molecular weight measurement. The identified peaks will also be tested for antiviral activities in vitro.


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