Primers for Human Gene Transcripts of Varying Abundance Levels according
to the instructions.3
RNA of all abundance levels was detected
in samples isolated from 200 cells. High abundance RNA was detected in a
portion of the RT-PCR product equivalent to 2.5 cells, medium abundance
RNA from a portion equivalent to 10 cells, and low abundance RNA from
portions equivalent to 13 cells (Figure 1).
In addition, RNA isolated from malignant glioma cells using the
StrataPrep microprep kit has been used in differential display
experiments. These experiments identified several differentially
expressed genes in the invasive rim of the tumor relative to the
noninvasive tumor core.4
The StrataPrep total RNA microprep kit is ideal for the isolation of
RNA from small cell samples harvested by LCM. The method generates pure,
intact RNA with high efficiency that can be effectively amplified by
conventional and quantitative RT-PCR.
The authors thank Michael Berens, Neurology Research, Barrow
Neurological Institute, Phoenix, AZ for providing LCM samples.
1. Emmert-Buck, M. R., et al. (1996) Science 274:
2. Dolter, K. and Braman J. (2000) Strategies 13: 56-58.
3. Rogers, B. and McKenzie, D. (1998) Strategies 11: 100-102.
4. Berens, M. E., et al. (2000) Proceedings of the American
Association for Cancer Research 41:232, Abstract 1477.
Source:Page: All 1 2 Related biology technology :1
. Restriction Digests of DNA Isolated with the Perfectprep
Gel Cleanup Kit2
. A Better Way to Isolate RNA AND Protein From the Same Sample3
. Isolate It All: siRNA miRNA
Total RNA Native Protein4
. Isolate Total RNA and Protein From the Same Sample5
. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis6
. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System7
. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module8
. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 10009
. The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies10
. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 300011
. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000