Navigation Links
Isolate and Analyze Total RNA from Cells Harvested by Laser Capture ,,, Microdissection

enes RT-PCR Primers for Human Gene Transcripts of Varying Abundance Levels according to the instructions.3 RNA of all abundance levels was detected in samples isolated from 200 cells. High abundance RNA was detected in a portion of the RT-PCR product equivalent to 2.5 cells, medium abundance RNA from a portion equivalent to 10 cells, and low abundance RNA from portions equivalent to 13 cells (Figure 1).

In addition, RNA isolated from malignant glioma cells using the StrataPrep microprep kit has been used in differential display experiments. These experiments identified several differentially expressed genes in the invasive rim of the tumor relative to the noninvasive tumor core.4

Conclusions

The StrataPrep total RNA microprep kit is ideal for the isolation of RNA from small cell samples harvested by LCM. The method generates pure, intact RNA with high efficiency that can be effectively amplified by conventional and quantitative RT-PCR.

Acknowledgments

The authors thank Michael Berens, Neurology Research, Barrow Neurological Institute, Phoenix, AZ for providing LCM samples.

REFERENCES

1. Emmert-Buck, M. R., et al. (1996) Science 274: 998-1001.
2. Dolter, K. and Braman J. (2000) Strategies 13: 56-58.
3. Rogers, B. and McKenzie, D. (1998) Strategies 11: 100-102.
4. Berens, M. E., et al. (2000) Proceedings of the American Association for Cancer Research 41:232, Abstract 1477.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
2. A Better Way to Isolate RNA AND Protein From the Same Sample
3. Isolate It All: siRNA miRNA Total RNA Native Protein
4. Isolate Total RNA and Protein From the Same Sample
5. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
6. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
8. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
9. The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies
10. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
11. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
Post Your Comments:
(Date:4/16/2015)... Carolina (PRWEB) April 17, 2015 ... steam system services, released their newly designed, state-of-the-art ... The customer facing website effectively promotes the Spirax ... , All the information that a site visitor ... system services, company capabilities, industries serviced, the latest ...
(Date:4/16/2015)... CA (PRWEB) April 16, 2015 ... the Buzz of BIO Contest for the ... Organization (BIO), the 2015 BIO International Convention will take ... Philadelphia, Pennsylvania. During the Buzz of BIO contest, industry ... to see present at the conference. The community voting ...
(Date:4/16/2015)... The Council for Agricultural Science ... Borlaug CAST Communication Award goes to Dr. Channapatna ... in Alabama. Award nominees must demonstrate an ability ... the use of television, radio, or other social ... a specialist in the field of plant genetics ...
(Date:4/16/2015)... MOSCOW , April 16, 2015 /PRNewswire/ ... (ChemRar), the Russian pharmaceutical investment and R&D ... announced collaboration for research, development and commercialization ... with the intention to stimulate ... industry.      (Logo: http://photos.prnewswire.com/prnh/20150416/739742-a) ,     ...
Breaking Biology Technology:Spirax Sarco website has a new look 2Proove Bisociences to Participate in the Buzz of BIO at the 2015 BIO International Convention 2Proove Bisociences to Participate in the Buzz of BIO at the 2015 BIO International Convention 3The 2015 Borlaug CAST Communication Award Goes to Channapatna Prakash 2Lancet and ChemRar High Tech Center Have Announced a Partnership in the Development and Production of Novel Drugs in Russia 2
... Andrew Conn, Lindy Durrant, and Ian Spendlove, Cancer Research,Campaign Academic Unit, Nottingham City Hospital, ... Introduction , ... and reproducible , method of DNA vaccine ... protective immunity in animal models to both infectious diseases and ...
... and Professor Norbert Nowotny, PhD, Clinical , Virology ... Vienna , Veterinaerplatz 1, A 1210 Vienna, Austria , ... , The purity of the DNA template ... the most critical factor in nucleotide sequencing. The presence of , ...
... Marttinen*, H.-L. Malinen*, V.-P. Jaakola** and , T. Alatossava* ... * Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, FIN-88600 , ... Environmental Science, University of Jyvskyl, , PL 35, FIN-40351 Jyvskyl, ... Introduction , β-lactoglobulin (βLG) is a major ...
Cached Biology Technology:Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice 2Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice 3Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice 4Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice 5Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice 6Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice 7Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice 8Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A 2Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A 3Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A 4Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 2Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 3Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 4Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 5Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 6Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 7Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 8Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 9Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 10Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 11Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing 12
(Date:3/26/2015)... 26, 2015 The Granite Club, ... and athletic club, today announced it has implemented a ... of movement for members and staff, while restricting access ... process, we selected FST,s IMID Access system because it ... our members and staff, in addition to unparalleled security," ...
(Date:3/23/2015)... -- SoundView Technology Group issues a new research update following ... smart wallet. SoundView was one of the selected user groups to ... Wocket in multiple scenarios and outlets. Soundview ... making both debit and credit card payments.  ... company meets their plans in 2015, it would push our ...
(Date:3/20/2015)... , Mar. 19, 2015 Research and Markets ... "Hand Geometry - Global Strategic Business Report" report ... markets for Hand Geometry in US$ Thousands. The report provides ... , Japan , Europe ... , and Rest of World. Annual estimates ...
Breaking Biology News(10 mins):Granite Club First Private Club in North America to Implement FST Biometrics Secure Access Solution, as Part of Multi-million Dollar Expansion 2Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 2Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 3Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 4Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 2Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 4
... the self-assembly of nanoparticles is a fundamental question in ... way to make nanocrystals in a fluid assemble into ... in living organisms. , "This establishes an ... and nanotechnology, that is, proteins and nanoparticles, and this ...
... most of the work in living cells, and the DNA ... proteins. The crucial job of reading the genetic instructions and ... protein factories humming away inside the cells of all living ... at the University of California, Santa Cruz, has been studying ...
... the sleek and efficient propulsion of squid, jellyfish and ... has designed a new generation of compact vortex generators ... and dock underwater vehicles at low speeds and with ... inspired by the plots of two classic sci-fi films ...
Cached Biology News:Researchers make nanosheets that mimic protein formation 2Biologists probe the machinery of cellular protein factories 2Biologists probe the machinery of cellular protein factories 3Squid-inspired design could mean better handling of underwater vehicles 2
RNA oligomers from 10 to 50 bases purified by PAGE and MS-checked. RNAs are supplied dried and fully deprotected....
... The iEMS Incubator/Shaker HT ... Thus, the iEMS Incubator/Shaker HT ... as DNA hybridization and primer ... HT is available as a ...
... a modified cap analog in which the ... replaced with OCH3. Because of this substitution, ... with the remaining hydroxyl group thus forcing ... a result, unlike transcripts synthesized with conventional ...
...
Biology Products: