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Isolate Total RNA and Protein From the Same Sample

obe 5' labeled and purified with the mirVana Probe & Marker Kit (Ambion). GAPDH protein levels were analyzed as in Figure 2. Real time PCR reactions were performed in quadruplicate using TaqMan primer and probe sets for 18S rRNA and GAPDH mRNA (Applied Biosystems).

Figure 4. Summary of Real Time PCR Results. Average Ct values, the range of Ct values, and standard deviations are given for the replicate samples used in real time RT-PCR experiments in Figures 2 and 3.


Powerful System

The mirVana PARIS Kit is a robust method ideal for quantification of protein and RNA, including small RNA species. The kit contains sufficient reagent for 40 protein and RNA isolations; each purification can accommodate 100 to 107 cells or 1 to 100 mg of tissue. The entire procedure is fast and easy, isolating RNA and proteins in less than 30 min. Also, the mirVana PARIS Kit is compatible with samples treated with RNAlater (Ambion), a reagent that stabilizes RNA within tissue prior to processing. For more information about the mirVana PARIS Kit and other tools dedicated to small RNA analysis, please visit www.ambion.com/miRNA.


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