Navigation Links
Isolate Total RNA and Protein From the Same Sample

//www.ambion.com/catalog/CatNum.php?">Vana miRNA Detection Kit [2, 3], miR-16 miRNA could barely be detected in 1 g of total RNA isolated from HeLa cells in this way. In contrast, RNA isolated using both the mirVana miRNA Isolation Kit and mirVana PARIS Kit procedures included these small RNA species.

Figure 1. Differential Recovery of Small RNAs. Total RNA was isolated from 1x106 HeLa cells with the indicated kit as per protocol. One g of total RNA was resolved on a 15% denaturing acrylamide gel and stained with EtBr (top) or analyzed by solution hybridization assay with the mirVana miRNA Detection Kit (Ambion) and a probe specific for miR-16 prepared by in vitro transcription with the mirVana Probe Construction Kit (Ambion). Gel was exposed for 6 (middle) or 24 (bottom) hours at -80C


Measuring Reproducibility

Variation in protein, mRNA, and/or small RNA recovery can be an issue if precise quantitation is required in downstream experiments (e.g. RNAi experiments, miRNA profiling). Therefore, reproducible, quantitative recovery of RNA and protein is critical. To determine the reproducibility of the mirVana PARIS procedure, both RNA and protein were isolated from replicates of two types of biological samples, and various quantitative and qualitative parameters were measured. The experimental samples tested were human cell lines assayed 2 days after plating (12 replicates, Figure 2), and mouse liver tissue kept frozen for 2 months at -80C prior to assay (6 replicates, Figure 3). For each experiment, total RNA and protein yields were determined, and samples were assessed for effi
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
2. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
3. A Better Way to Isolate RNA AND Protein From the Same Sample
4. Isolate It All: siRNA miRNA Total RNA Native Protein
5. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
6. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
7. High-Throughput Isolation of Total RNA
8. Fast Isolation of Total RNA from Small Numbers of Cells
9. Total RNA Isolation Protocol 1,2,3
10. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
11. Total RNA from Whole Blood for Expression Profiling
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Isolate Total RNA and Protein From the Same Sample

(Date:8/1/2014)... 2014 For the past five ... the auspices of the Armed Forces Institute of ... academic research organizations in the development of advanced ... is a multi-institutional, inter¬disciplinary network working to develop ... suffered by US servicemen and women in the ...
(Date:8/1/2014)... the photonic analog of topological electron flow in certain ... around the edge of the material but not through ... if electrons encounter impurities in the material the electrons ... In the photonic equivalent, light flows ... a meta-material consisting of an array of tiny glass ...
(Date:8/1/2014)... Chantel Lee ’15 and Eli Capello ’15 sacrificed a leisurely ... Juan Rodriguez, Professor of Physics, in an effort to combat ... caused by changes in the blood vessels of the retina. ... remarkable,” said Rodriguez. “They took on a graduate level project, ... carried it to near completion in just a few short ...
(Date:8/1/2014)... (PRWEB) August 01, 2014 Applied ... provider of DNA-based anti-counterfeiting technology and product authentication solutions, ... 2014 to discuss its fiscal third quarter results. The ... the SEC by the end of the day on ... Inc. management will host a conference call beginning at ...
Breaking Biology Technology:Wounded Warriors a Focus of the 12th New Jersey Symposium on Biomaterials Science 2On-chip topological light 2On-chip topological light 3Two Centenary College Students Tackle Diabetic Retinopathy Research 2Applied DNA Sciences to Host Conference Call Tuesday August 12, 2014 2Applied DNA Sciences to Host Conference Call Tuesday August 12, 2014 3
... a private,biotechnology company and leading developer of clean ... Singapore,Pte. Ltd. (CLS). CLS is the company,s first ... the company,s research and development operations in the,U.S. ... business in,both the innovator and generics markets. ...
... Inc. (Amex: PLX ), announced today the pricing ... common stock at a,public offering price of $5.00 per ... underwriters to purchase up to 1,500,000 additional shares of,common ... Bank is acting as sole book-running manager for this,offering. ...
... Inc. (Nasdaq: HGSI ) today announced that it ... for the quarter ended,September 30, 2007. These results are ... (Logo: http://www.newscom.com/cgi-bin/prnh/20010612/HGSLOGO ) The conference call will ... Thursday, November 1, 2007, at 11:00 am Eastern Time. ...
Cached Biology Technology:Codexis Opens Singapore Laboratory 2Protalix BioTherapeutics Announces Pricing of Public Offering of Common Stock 2Human Genome Sciences To Sponsor Conference Call To Discuss Third Quarter 2007 Financial Results 2
(Date:8/1/2014)... As a quantum state collapses from a quantum superposition to ... a path known as a quantum trajectory. For each start ... path, but it is not as easy to predict the ... points would be in our everyday, classical world. , In ... Nature , scientists from the Institute for Quantum Studies ...
(Date:8/1/2014)... reptile species have been recorded into the Reptile ... species and classification, making the reptile species among ... alongside bird and fish species. , For some ... the year that reptiles would become the most ... snakes, lizards, turtles, crocodiles, tuataras and amphisbaenians. , ...
(Date:8/1/2014)... fossil record about 200,000 years ago, but it was ... advanced tools became widespread. , A new study ... finds that human skulls changed in ways that ... same time that culture was blossoming. , "The modern ... cultural exchange probably came at the same time that ...
Breaking Biology News(10 mins):Mapping the optimal route between two quantum states 2Reptile Database surpasses 10,000 reptile species 2Reptile Database surpasses 10,000 reptile species 3Society bloomed with gentler personalities and more feminine faces 2
... Without aggressive action to reduce soot emissions, the time ... be significantly accelerated in order to achieve international climate ... December,s Copenhagen Accord, according to " Assessing the climatic ... online June 21 in the Proceedings of the National ...
... product is organic, it,s also environmentally friendly. ... organic pesticides can have a higher environmental impact ... require larger doses. Environmental sciences professor Rebecca ... effectiveness and environmental impact of organic pesticides to ...
... freshwater fishes has created a great home for parasites, according ... . The study provides a lens into the evolutionary world ... probably has the best studied freshwater fish parasites in the ... more species of flukes in a few fishes from the ...
Cached Biology News:Aggressive action to reduce soot emissions needed to meet climate change goals 2Aggressive action to reduce soot emissions needed to meet climate change goals 3Organic pesticides not always 'greener' choice, study finds 2Adaptation is (not) in the eye of the beholder 2
... Software reads and processes raw ... microarray data for analysis. ... one 2-year license for Feature ... , * one CD-ROM ...
... and SYBR Safe DNA Gel Stain combo offers these ... buying these same products separately ... bromide Eliminates risks to ... and RNA fragments from 100 bp to >30 kb. ...
Anti-WT-1 Liquid. In 10 μM PBS, 0.2 % BSA, pH 7.6. Recognizes the ~47-55 kDa Wilm's tumor protein in Wilm's tumor tissue. ...
... The economical ICS-90 uses continuous chemical ... to give you fast, accurate determinations of ... ranges. Easy to operate, quick to equilibrate, ... the compact ICS-90 helps you achieve maximum ...
Biology Products: