( mirVana PARIS Kit--Optimized for...)Isolate Total RNA and Protein From the Same Sample

mirVana PARIS Kit--Optimized for Recovery of siRNA and miRNA

Ambion's mirVana PARIS Kit combines the technological advances from both the mirVana miRNA Isolation Kit, optimized for small RNA recovery, and the PARIS (Protein And RNA Isolation System) Kit, specifically developed to isolate protein and RNA from the same experimental sample (see MicroRNAs: Isolation to Functional Analyses). The result is a versatile kit that provides quantitative recovery of native protein and all RNA species, including siRNA and miRNAs. The kit is ideal for downstream assessment of protein and mRNA expression levels, analysis of microRNA (miRNA) expression patterns, and correlation of siRNA expression with protein and mRNA knockdown in RNAi experiments [1].

Reproducible, Quantitative Recovery of Protein and RNA

There is a direct relationship between the inefficient recovery of 5S rRNA and the loss of smaller RNA species, such as miRNAs and siRNAs, during total RNA isolation [2]. For example, total RNA samples prepared with an RNA isolation procedure not optimized for small RNA recovery lack RNA species smaller than ~200 nt, including 5S rRNA, tRNA, and miRNA (Figure 1, PARIS lane). Even with the sensitivity of the mir

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