Navigation Links
Isolate It All: siRNA miRNA ,,, Total RNA Native Protein

ring sample homogenization, total RNA was isolated from four different mouse tissues with the mirVana PARIS Kit or the mirVana miRNA Isolation Kit. With the latter kit, tissue samples are homogenized directly in a solution containing a chaotropic denaturant, which reduces the opportunities for RNA degradation.

Comparison of the purified RNA on a denaturing gel showed no significant RNA degradation with either kit (Figure 5A). Most importantly, analysis of small RNA expression profiles showed no difference between the two procedures. let-7 microRNA was more abundant in brain than kidney, liver, or thymus, whereas both 5S and 5.8S rRNA were detected at the same level in all 4 tissues. As previously reported [3, 4], miR-124 was expressed only in brain. Together, these results show that homogenization in Cell Disruption Buffer prior to addition of chaotropic denaturant does not significantly affect RNA quality. The advantage is that this lysate contains native protein that can be used directly in a host of downstream applications [2, 5], such as two-dimensional gel electrophoresis (Figure 5B).

Figure 5. Isolation of Total RNA and Protein from Different Tissues. Total RNA and protein were isolated from 4 different mouse tissues (~50 mg) with the mirVana miRNA Isolation Kit (RNA only) or the mirVana PARIS Kit. (A) Small RNA analysis. One g of total RNA was analyzed by denaturing gel and Northern blot as described in Figures 2 and 3.(B) Two-dimensional gel analysis. About 125 g of total protein from mouse brain was resolved using a pH 4-7 IPG gel followed by a 8-1
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
2. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
3. A Better Way to Isolate RNA AND Protein From the Same Sample
4. Isolate Total RNA and Protein From the Same Sample
5. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
6. Custom and library siRNA for efficient gene silencing
7. Custom and library siRNA for efficient gene silencing
8. Cancer siRNA Oligo Set Version 1.0
9. Library siRNA
10. Custom siRNA Oligo Synthesis Service
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Isolate All siRNA miRNA Total RNA Native Protein

(Date:5/20/2013)... 20, 2013   Flexion Therapeutics , Inc. today ... to its management team as chief financial ... financial management experience with biotechnology and medical device companies. ... chief financial officer for Novavax (NASDAQ: NVAX ... than $250 million through a combination of investment funding ...
(Date:5/20/2013)... Interventional cardiologist Tony Farah, MD, and ... Cardiovascular Institute have helped pioneer many breakthroughs ... over the past three decades, from new disease-fighting ... coronary artery stent implantation. , Today, AGH officials announced ... role in the study of a novel, first-of-its kind ...
(Date:5/20/2013)... Recognizing exciting and innovative scientific research, ... 2013 Class of Young Professors. Over the ... outstanding international group with more than $824,000 to ... meet global challenges.     , The DuPont Young Professor ... untenured research faculty working in areas of interest ...
(Date:5/20/2013)... Kitware, a leader in data visualization, today ... exploration of hospital costs across the United States ... recent release of “Medicare Provider Charge Data,” a dataset ... from the Centers for Medicare & Medicaid Services. , ... hospitals across the country has sparked considerable controversy; however, ...
Breaking Biology Technology:Flexion Therapeutics Names Frederick Driscoll Chief Financial Officer 2Allegheny General Hospital Cardiovascular Institute Becomes Exclusive Pittsburgh Site for Study of Novel Coronary Artery Disease Device 2Allegheny General Hospital Cardiovascular Institute Becomes Exclusive Pittsburgh Site for Study of Novel Coronary Artery Disease Device 3Allegheny General Hospital Cardiovascular Institute Becomes Exclusive Pittsburgh Site for Study of Novel Coronary Artery Disease Device 4DuPont Celebrates Scientific Innovation by Recognizing Young Professors 2DuPont Celebrates Scientific Innovation by Recognizing Young Professors 3Kitware Enables Interactive Exploration of CMS Medicare Data 2
... UltraTape Industries, a division,of Delphon Industries and ... today the availability of sample kits for ... are currently 5,sample kits available including those ... Tapes, ESD Tapes, Medical and,Pharmaceutical Tapes, and ...
... CLARA, Calif., April 22 /PRNewswire/,-- Agendia BV, a ... molecular,diagnostics, and Agilent Technologies, Inc. (NYSE: A ... collaborate to develop new in-vitro,diagnostic tests. In addition, ... products will continue to be supplied on Agilent ...
... $250,000 under March ... ... BMRX), a leading developer and supplier of consumer-based,finger-activated products, today announced ... personnel of Sequiam,Corporation (OTC Bulletin Board: SQUME) through a previously announced,agreement ...
Cached Biology Technology:UltraTape Announces Sample Kits Available for Purchase Through Website 2Agendia and Agilent Announce Plans to Jointly Develop New Diagnostic Tests, Extend Supply Agreement 2Agendia and Agilent Announce Plans to Jointly Develop New Diagnostic Tests, Extend Supply Agreement 3bioMETRX, Inc. Initiates Integration of Personnel and Assets of Sequiam Corp. 2
(Date:5/16/2013)... Illustrating a commitment to the support of ... (AGA) Research Foundation has announced the inaugural AGA ... recipients. Supported by the National Institute of Diabetes ... award helps underrepresented minority students to further their ... , "By establishing this new award, AGA demonstrates ...
(Date:5/16/2013)... ancient shorelines to predict the stability of today,s largest ice ... from three million years ago, for example when Earth ... be evidence of a high sea level due to ice ... scientists to think that if the world,s largest ice sheets ... same in our modern, progressively warming world. , However, ...
(Date:5/16/2013)... 2013 The relationship between the heritable risk ... been clear. Schizophrenia is commonly associated with cognitive ... clues that reduced IQ may be linked to ... cognitive ability may precede the onset of schizophrenia ... healthy relatives of people diagnosed with schizophrenia. , ...
Breaking Biology News(10 mins):Underrepresented minority students receive fellowships in digestive disease and nutrition research 2World's biggest ice sheets likely more stable than previously believed 2Genetic risk for schizophrenia is connected to reduced IQ 2
... Innes Centre in Norwich have discovered how roots find their ... in the forthcoming edition of Science, also explains how germinating ... burrow. The key is in the fuzzy coat of ... We have identified a growth control mechanism that enables these ...
... important discovery has been made with respect to the mystery ... research professor at Arizona State University, found that some of ... Earth have been shown to carry handedness in a larger ... in this weeks Early Edition of the Proceedings of the ...
... technique that combines low temperature measurements and theoretical calculations, ... for the first time the electronic structure of single ... properties of DNA is an important issue in many ... in the study of DNA damage by ultraviolet radiation ...
Cached Biology News:ASU researcher may have discovered key to life before its origin on Earth 2Electronic structure of DNA revealed for 1st time by Hebrew University and collaborating researchers 2
... is an affordable, compact benchtop imager ... providing a broad array of automated ... high-resolution images with high-performance laser autofocus, ... and emission filter changers, and a ...
... include: Microarray hybridization chamber assembly (1 ... clamp, thumbscrew) Microarray chamber user guide ... intended to be cleaned and reused. The ... Catalog Oligo Microarray kits and all custom ...
... Blood Vessel Staining Kit is a very ... vascular endothelial cells, containing both anti-von Willebrand ... applicable to a variety of species. This ... for the staining of frozen and paraffin-embedded ...
... Ham's F-12 was developed for culturing hybrid ... The modification consists of doubling the amino ... The salt concentrations have been altered as ... sulfate per liter, which may render it ...
Biology Products: