Navigation Links
Isolate It All: siRNA miRNA ,,, Total RNA Native Protein

timized procedures; Ambion's mirVana miRNA Isolation Kit was specifically optimized for efficient isolation of RNA species smaller than 200 nt [1]. To develop the mirVana PARIS Kit, this technology was adapted and combined with the advantages of the PARIS Kit, the first commercially available kit designed to isolate both RNA and native protein from the same sample [2]. The result is a versatile kit for quantitative recovery of native protein and all RNA species, including small RNAs. A summary of each kit's specification is presented in Figure 1, and specific examples are provided below.


Figure 1. Which Kit to Choose?


Recover High Quality Total RNA
To assess the quality of total RNA isolated with the mirVana PARIS Kit, 1 x 106 HeLa cells were homogenized in the Cell Disruption Buffer provided with the kit. Because the homogenization is performed quickly on ice and in the presence of detergent, both protein and RNA can be recovered from the lysate. A fraction of the lysate can be used directly to analyze protein expression (e.g. Western blot, 2D gel electrophoresis) or for assays that require native protein (e.g. functional assay, reporter activity). For RNA isolation, the HeLa cell lysate was immediately mixed with an equal volume of 2X Denaturing Solution. This solution contains a high concentration of chaotropic denaturant
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
2. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
3. A Better Way to Isolate RNA AND Protein From the Same Sample
4. Isolate Total RNA and Protein From the Same Sample
5. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
6. Custom and library siRNA for efficient gene silencing
7. Custom and library siRNA for efficient gene silencing
8. Cancer siRNA Oligo Set Version 1.0
9. Library siRNA
10. Custom siRNA Oligo Synthesis Service
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Isolate All siRNA miRNA Total RNA Native Protein

(Date:8/27/2015)... -- The National Necrotizing Fasciitis Foundation (NNFF) ... doctors at Vanderbilt University Hospital in Nashville, ... Dr. John Crew , director of the ... in Daly City, CA. ... by Dr. Crew, that had previously saved the lives ...
(Date:8/27/2015)... /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the Company") today ... 30, 2015. Biorem,s complete second quarter financial statements and MD&A have ... ,000,s except earnings per shareThree-months ended , Six -months endedJune 30 ... 2015 , 2014 Revenue , 4,709 , ... profit , 1,656 , 56 , ...
(Date:8/27/2015)... 27, 2015 GlassesOff (OTCBB: GLSO), a ... in the human vision system, announced today a partnership with ... Chris Paul to develop a new mobile app ... improve their real-life on-court performance. Vision is the ... is shooting a ball or blocking a pass. The critical ...
(Date:8/27/2015)... ... August 27, 2015 , ... ... needs and expertly narrows down the product choices to a manageable shortlist of ... down the list to models that fit best with the customer’s industry and ...
Breaking Biology Technology:Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 3BIOREM Reports $718,000 Net Earnings for the Second Quarter 2BIOREM Reports $718,000 Net Earnings for the Second Quarter 3Eight-Time All-Star Chris Paul and GlassesOff Partner to Develop Innovative Mobile App Designed to Improve On-Court Performance 2Eight-Time All-Star Chris Paul and GlassesOff Partner to Develop Innovative Mobile App Designed to Improve On-Court Performance 3METTLER TOLEDO Updates Online Scale Selection Tool 2
... Nov. 3 Altogen Biosystems (http://www.altogen.com ),announced ... laboratories and research facilities for animal testing. Transfection ... introduce DNA and RNA molecules,into cells and tissues. ... barriers and deliver a gene or a small ...
... Nov. 3 Nile Therapeutics,Inc., (Nasdaq: NLTX ), ... at the following upcoming investor conferences:, -- Rodman ... ET,in the Kennedy II room on Monday, November 10, ... -- Lazard Capital Markets 5th Annual Healthcare Conference at ...
... Metabolex, Inc., a,biopharmaceutical company focused on the ... the treatment of metabolic diseases,announced that two ... been,recognized in Windhover,s "Top 10 most interesting ... MBX-2982, a potential,first-in-class treatment for type 2 ...
Cached Biology Technology:Altogen Biosystems Launches New Nanoparticle-based In Vivo Transfection Reagent 2Nile Therapeutics to Present at Two Upcoming Investor Conferences 2Two Metabolex Projects Recognized in Windhover's Top 10 Lists for Partnering Opportunities 2Two Metabolex Projects Recognized in Windhover's Top 10 Lists for Partnering Opportunities 3
(Date:8/4/2015)... N.Y., Aug. 4, 2015  AMRI (NASDAQ: AMRI ) today ... 30, 2015. Highlights: , Second quarter contract ... from 2014 , Adjusted contract margins of ... of $0.22, including a $0.05 decrease in EPS from royalties in ... $16 million "We are very pleased to present ...
(Date:7/31/2015)... 2015 BGI llevará a cabo la 10ª Conferencia ... 25 de octubre de 2015, en Shenzhen, ... décimo aniversario este año. Desde su inauguración en 2006, ... anuales más influyentes del mundo en los campos de ... más dinámicas, entusiastas y amenas. ICG-10 se ...
(Date:7/31/2015)... de julio de 2015 La 10 th ... por medio de BGI del 22 al 25 de octubre de ... Este año, la conferencia celebra su décimo aniversario. Desde ... en una de las reuniones anuales más influyentes del mundo ... reuniones más dinámicas, entusiastas y mejores a nivel científico. ...
Breaking Biology News(10 mins):AMRI Announces Second Quarter 2015 Results 2AMRI Announces Second Quarter 2015 Results 3AMRI Announces Second Quarter 2015 Results 4AMRI Announces Second Quarter 2015 Results 5AMRI Announces Second Quarter 2015 Results 6AMRI Announces Second Quarter 2015 Results 7AMRI Announces Second Quarter 2015 Results 8AMRI Announces Second Quarter 2015 Results 9AMRI Announces Second Quarter 2015 Results 10AMRI Announces Second Quarter 2015 Results 11AMRI Announces Second Quarter 2015 Results 12AMRI Announces Second Quarter 2015 Results 13AMRI Announces Second Quarter 2015 Results 14AMRI Announces Second Quarter 2015 Results 15AMRI Announces Second Quarter 2015 Results 16AMRI Announces Second Quarter 2015 Results 17AMRI Announces Second Quarter 2015 Results 18AMRI Announces Second Quarter 2015 Results 19AMRI Announces Second Quarter 2015 Results 20AMRI Announces Second Quarter 2015 Results 21AMRI Announces Second Quarter 2015 Results 22AMRI Announces Second Quarter 2015 Results 23AMRI Announces Second Quarter 2015 Results 24La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 3La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 2La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 3
... New research shows that a recently discovered enzyme that destroys ... to protect the mRNA during times of stress. The response ... , The study examined a recently discovered enzyme called PMR1. ... like a hand grenade with its pin in place. ...
... intricate structure of cartilage in what they hope will provide ... day be treated. , The disease, which affects more than ... wear and tear of the smooth, hard cartilage tissue that ... one another at the joint. , Scientists have long ...
... Exposure to phthalates, a common chemical found in everything ... reproductive problems and now, for the first time, is ... males, according to a study by the University of ... growing suspicion that low-dose exposures to phthalates and other ...
Cached Biology News:Destructive enzyme shows a benevolent side 2Cartilage discovery offers arthritis hope 2Phthalates now linked to fat, related health risks 2
Immunogen: Synthetic peptide (aa sequence is considered to be commercially sensitive) Storage: -20 C, Avoid Freeze/Thaw Cycles...
... Immunogen: Fusion protein derived from ... ASIP/PAR-3 (atypical PKC isotype-specific interacting protein/ ... not related to the protease-activated-receptors also ... Xenopus (positive controls: Caco-2 MDCK A6 ...
... C-1-tetrahydrofolate synthase, ... [Includes: Methylenetetrahydrofolate dehydrogenase ... cyclohydrolase (EC 3.5.4.9); ... 6.3.4.3)]. [Source:Uniprot/SWISSPROT;Acc:P11586] ...
...
Biology Products: