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Isolate It All: siRNA miRNA ,,, Total RNA Native Protein

Emmanuel Labourier, Ambion, Inc


Isolate total RNA and native protein from the same sample
Enrich for small RNAs (<200 nt)
Ideal for assessing siRNA or miRNA expression and processing

Small interfering RNAs (siRNAs) and microRNAs (miRNAs) have emerged as powerful post-transcriptional regulators of gene expression in many different organisms, thus making the analysis of small RNA molecules increasingly important. In addition to monitoring small RNA expression, analysis of protein expression levels is critical for thorough analysis of the effects of small RNAs. In RNAi experiments, for example, exogenously introduced siRNAs are used to target the degradation of specific messenger RNAs (mRNAs), resulting in gene knockdown at both the mRNA and the protein level. In contrast, miRNAs are endogenous 21-24 nt RNAs that primarily act as repressors of translation and therefore affect only protein expression levels. Here we discuss a procedure for downstream monitoring of protein, mRNA, siRNA, and miRNA expression levels from the same experimental sample.


Two Kits in One
To isolate protein, small RNA and long RNA species from the same sample, Ambion scientists have developed a unique tool: the mirVana PARIS Kit. Quantitative recovery of small RNA during total RNA isolation requires op
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