Navigation Links
Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis

RNAqueous-Micro Kit Ideal for Processing Laser Capture Microdissection Samples


Further development of Ambion's RNAqueous-Micro Kit has resulted in a 3-6 fold increase in total RNA yields from LCM samples. In addition, Ambion has introduced a new LCM Staining Kit that has been optimized to avoid the RNA degradation issues commonly seen during conventional staining protocols. LCM tissues processed with Ambion's new LCM Staining Kit, followed by total RNA isolation using the improved RNAqueous-Micro Kit, generates high quality total RNA suitable for amplification with Ambion's MessageAmp II aRNA Amplification Kit. It is now possible to carry out microarray expression profiling and qRT-PCR using limited quantities of total RNA recovered from LCM samples. The experiments below illustrate the combined use of these Ambion products to enable expression profiling from LCM-microdissected brain samples.


Higher RNA Recovery from LCM Samples

The ability to analyze global expression profiles or specific gene expression levels from microdissected samples is becoming increasingly important for understanding biological processes. While the RNAqueous-Micro Kit has always been ideal for isolating total RNA from microscale samples (<5 x 105 cultured cells and <10 mg tissue), Ambion scientists have now optimized its use for RNA recovery from microdissected samples. The improved kit shows a significant increase in
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
2. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
3. A Better Way to Isolate RNA AND Protein From the Same Sample
4. Isolate It All: siRNA miRNA Total RNA Native Protein
5. Isolate Total RNA and Protein From the Same Sample
6. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
7. Generate High-Quality, Directional Plasmid cDNA Libraries
8. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
9. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.
10. High Quality RNA Isolation Services
11. Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix
Post Your Comments:
(Date:8/27/2015)... RESEARCH TRIANGLE PARK, N.C. , Aug. 27, ... reports on the decision by FedEx, UPS and ... shipments of certain biological specimens classified as ,select ... expertise and long history of handling these sensitive ... network, knowledge of global transportation regulations, trained and ...
(Date:8/26/2015)... ... August 26, 2015 , ... ... investment in the development and manufacture of highly valued cardiac markers used in ... team's extensive expertise with protein chemistry has led to the development and commercialization ...
(Date:8/26/2015)... 2015 China Cord Blood Corporation (NYSE: ... leading provider of cord blood collection, laboratory testing, hematopoietic ... announced that the Company has been informed that Magnum ... owned by Mr. Yuen Kam, chairman of the board ... shares (the "Shares") of  Excellent China Healthcare Investment Limited ...
(Date:8/25/2015)... SHANGHAI , Aug. 25, 2015   WuXi ... open-access R&D capability and technology platform company serving the ... Holdings Ltd. ("Lee,s Pharm," Hong Kong Exchange Stock Code: ... Hong Kong with 20 years of operations ... the two companies have signed an agreement whereby WuXi,s ...
Breaking Biology Technology:Marken Ensures Safe Transportation Of Select Agents And Toxins 2Lee Biosolutions Announces Increased Investment in the Development and Manufacturing of Biomarkers Used in Early Detection Of Heart Disease 2China Cord Blood Corporation Receives Notice of Transactions Regarding its 7% Senior Convertible Notes Due 2017 2China Cord Blood Corporation Receives Notice of Transactions Regarding its 7% Senior Convertible Notes Due 2017 3WuXi PharmaTech to Be Exclusive Supplier of Laboratory Testing Services to Lee's Pharm 2WuXi PharmaTech to Be Exclusive Supplier of Laboratory Testing Services to Lee's Pharm 3
... - Dr. Brad Thompson, President,and CEO of Oncolytics ... a,corporate overview of the company at Toronto CFA ... being held at the National Club, 303 Bay,Street, ... Inc. Oncolytics is a Calgary-based biotechnology company ...
... Nov. 15 Imaging,Diagnostic Systems, Inc., (OTC Bulletin Board: ... announced that its CT Laser,Mammography (CTLM(R)) system, designed as ... its future clinical applications were featured in,a cover story ... magazine. This,major article was contributed by Eric Milne, MD, ...
... Nov. 14 Advanced Life Sciences,Holdings, Inc. (Nasdaq: ... the,discovery, development and commercialization of novel drugs in ... today,announced its financial results for the third quarter ... The net loss for the three months ...
Cached Biology Technology:Media Advisory - Oncolytics Biotech Inc. to Present at Toronto CFA Society 2New Optical Breast Cancer Imaging Technology Garners Cover Story in National Radiology Magazine 2New Optical Breast Cancer Imaging Technology Garners Cover Story in National Radiology Magazine 3Advanced Life Sciences Announces Third Quarter 2007 Financial Results 2Advanced Life Sciences Announces Third Quarter 2007 Financial Results 3Advanced Life Sciences Announces Third Quarter 2007 Financial Results 4Advanced Life Sciences Announces Third Quarter 2007 Financial Results 5Advanced Life Sciences Announces Third Quarter 2007 Financial Results 6Advanced Life Sciences Announces Third Quarter 2007 Financial Results 7Advanced Life Sciences Announces Third Quarter 2007 Financial Results 8Advanced Life Sciences Announces Third Quarter 2007 Financial Results 9
(Date:8/20/2015)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" ... the growing mobile commerce market and creator of the ... Card Alliance and the EMV Migration Forum.  ... Card Alliance Latin America (SCALA) Chapter provides NXT-ID, Inc. ... leaders promoting adoption of smart card technology. ...
(Date:8/12/2015)... , Aug. 12, 2015   MedNet Solutions ... the entire spectrum of clinical research, is proud to ... the company,s comprehensive SaaS-based eClinical technology platform, has led ... the first two quarters of 2015.   Q2 2015 ... largest quarters measured by contract value sold in the ...
(Date:8/10/2015)... and TELTOW, Germany , August 10, ... (SMI), a world leader in Eye Tracking Technology for ... its OEM Eye Tracking Platform for integration into all ... contains reference designs for seamless integration of eye tracking ... reality HMDs and augmented reality smart glasses. Omnivision,s leading ...
Breaking Biology News(10 mins):NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 2NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 4MedNet Solutions Experiences Explosive Corporate Growth 2SMI Completes OEM Eye Tracking Platform to Cover All Consumer Displays 2SMI Completes OEM Eye Tracking Platform to Cover All Consumer Displays 3
... LA JOLLA, CAThe seeds sprouting in your spring garden may still ... a finite energy pack contained within each seed. Once those resources ... on a "green" photosynthetic program. Researchers at the Salk Institute for ... relayed. In a study published in the May 24, 2011, ...
... 18, 2011 The Initiative for a Competitive Inner City ... 100 list of the fastest-growing inner city companies in the ... year,s list.  The Inner City 100 program recognizes successful inner ... innovative business practices and job creation in America,s urban communities. ...
... A nationwide network to monitor and maintain honeybee health ... five-year, $5 million program funded by the U.S. Department ... led by Penn State. The Bee Informed Partnership ... management practices and use them to develop best practices ...
Cached Biology News:It's not easy being green 2It's not easy being green 3Prometheus Research Ranked #3 in 13th Annual ICIC and Fortune Magazine's Inner City 100 2Prometheus Research Ranked #3 in 13th Annual ICIC and Fortune Magazine's Inner City 100 3Penn State leads in honey bee health initiative 2
... is a 96-well ELISA-compatible plate (containing 12 x ... Monoclonal Antibody is immobilized by a method that ... the tail fiber protein of the T7 bacteriophage. ... 10 9 phage per well and low ...
... Telomerase Detection Kit is a ... for the fluorometric detection and ... activity. It incorporates refinements to ... adds the ability to quantitate ...
... The expertise that made the original Gene Pulser ... system available has been applied in the Gene ... The modular design of the Gene Pulser II ... accessory that has the widest range of settings ...
Caliper offers a wide array of standard LabChip products designed to be used in combination with our AMS 90 and LabChip 3000 microfluidic platforms....
Biology Products: