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Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis

RNAqueous-Micro Kit Ideal for Processing Laser Capture Microdissection Samples


Further development of Ambion's RNAqueous-Micro Kit has resulted in a 3-6 fold increase in total RNA yields from LCM samples. In addition, Ambion has introduced a new LCM Staining Kit that has been optimized to avoid the RNA degradation issues commonly seen during conventional staining protocols. LCM tissues processed with Ambion's new LCM Staining Kit, followed by total RNA isolation using the improved RNAqueous-Micro Kit, generates high quality total RNA suitable for amplification with Ambion's MessageAmp II aRNA Amplification Kit. It is now possible to carry out microarray expression profiling and qRT-PCR using limited quantities of total RNA recovered from LCM samples. The experiments below illustrate the combined use of these Ambion products to enable expression profiling from LCM-microdissected brain samples.


Higher RNA Recovery from LCM Samples

The ability to analyze global expression profiles or specific gene expression levels from microdissected samples is becoming increasingly important for understanding biological processes. While the RNAqueous-Micro Kit has always been ideal for isolating total RNA from microscale samples (<5 x 105 cultured cells and <10 mg tissue), Ambion scientists have now optimized its use for RNA recovery from microdissected samples. The improved kit shows a significant increase in
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