Navigation Links
Is RNA Amplification Necessary for Microarrays?

ether the individual genes that were differentially expressed in amplified samples would have been identified as such using total RNA. For this experiment, the number of "outliers" defined by authors as "A gene with expression ratios of greater than or equal to 2.0 or less than or equal to 0.5 in two reciprocal microarray analysis" identified using total RNA or amplified RNA (one or two rounds of amplification) were compared. The amplified samples identified approximately 80% of the genes selected as outliers with total RNA, with an 80% concordance between target made by one and two rounds of amplification. Thus amplification yielded approximately 2 times more outliers than total RNA, with 80% of these showing similar trends in expression between amplified and total RNA samples. Validation of the "outliers" between total RNA and amplified RNA was carried out using real-time PCR analysis. The PCR data suggested that the majority of outliers identified using amplified RNA are valid. Thus Feldman et al. also conclude that all RNA samples should undergo at least one round of amplification to maximize the quality of array data for detection of differentially expressed genes.

SYBR Green is a registered trademark of Molecular Probes, Inc.
Cy is a trademark of Amersham Biosciences.


back to top


Ordering Information
Cat
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Greater Amplification Specificity with New Hot Start PCR Enzyme
4. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
8. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
9. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
10. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
11. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNA Amplification Necessary for Microarrays

(Date:1/15/2014)... More than 5 million Americans are currently ... seniors will die with Alzheimer’s or another dementia, according ... shocked many Americans into looking for ways to improve ... age-related cognitive disorders. Jonathan Weisman, president of Biohack Pure, ...
(Date:1/15/2014)... NY (PRWEB) January 15, 2014 The Microcompetition ... a major disease. One of these latent viruses is the ... rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic inflammatory ... theory, a study found that RA patients have high concentrations ...
(Date:1/15/2014)... 2013 was a banner year of continued innovation ... continued independent research led by the team at Wake ... million grant from the Susanne Marcus Collins Foundation, had ... reviewed journal, Amy Grant highlighted Brainwave Optimization® in First ...
(Date:1/15/2014)... Toronto, ON (PRWEB) January 15, 2014 ... an experimental new therapy for the treatment of tinnitus. ... unique tinnitus frequency, and over a period of weeks to ... Notched Sound Therapy in two forms: Notched Music and Notched ...
Breaking Biology Technology:Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3
... / Electroporator 2510 , , , , , , , ... Protocol No. 4308 915.521 02/2002 , , , , ... , Microorganism , Mycobacterium intracellulare ... type , Bacteria, gram positive, , ...
... , , , , , , , ... Protocol No. 4308 915.046 11/2001 , , , , , ... , Cell line , L2, Schneider cells, Drosophila, ... pActin-SV-EGFP, , , ...
... , , , , , , Multiporator , , ... , Transfection Protocol , Protocol No. ... , , , , , ... cells (suspension cell line), , , ...
Cached Biology Technology:Mycobacterium intracellulare 2L2 2HL-60 2
(Date:4/23/2014)... source of a unique rhythmic sound, recorded for decades ... the Antarctic minke whale ( Balaenoptera bonaerensis ). First ... who thought it sounded like a duck, the bio-duck ... Southern Ocean, but its source has remained a mystery, ... of researchers deployed acoustic tags on two Antarctic minke ...
(Date:4/23/2014)... gas boom has transformed the energy landscape in ... could cause conflict among the energy industry, residents ... researchers. They add that degraded water quality is ... The feature article appears in the ACS journal ... S. Mauter and colleagues point out that a ...
(Date:4/23/2014)... Heidelberg, 23 April 2013 EMBO today announced Sophie ... winner of the 2014 EMBO Gold Medal. The award ... define the organization and development of the cell. , ... to understand cellular polarity, in particular the way in ... size and cell division. In the last 11 years, ...
Breaking Biology News(10 mins):Scientists identify source of mysterious sound in the Southern Ocean 2Scientists identify source of mysterious sound in the Southern Ocean 3How to avoid water wars between 'fracking' industry and residents 2EMBO Gold Medal 2014 awarded to Sophie Martin 2
... , , , , ... AUDIO: Falon Tilley and Michael W. Beets discuss the successful implementation of ... Click here for more information. , , ... , , , , , ...
... by Jonathan Waxman is a collection of short stories that ... a doctor,s life before the dawn of management. Written in ... from the author,s decades of clinical experience. Chutney Wars, The ... a few of the chapters in this engagingly written book. ...
... have been awarded fellowships from the Alfred P. ... NYU,s Center for Neural Science, and Nicholas Stavropoulos, ... NYU Langone Medical Center and its Neuroscience Institute. ... and scholars "whose achievements and potential identify them ...
Cached Biology News:Healthy Lunchbox Challenge helps influence healthy eating habits in children 2Healthy Lunchbox Challenge helps influence healthy eating habits in children 3Healthy Lunchbox Challenge helps influence healthy eating habits in children 4Two NYU faculty win Sloan Foundation research fellowships 2
... polyclonal antibody raised against a ... Immunogen: K6HF (NP_004684, ... partial recombinant protein with GST ... Number: NM_004693 ...
... The binding specificity and signaling of the ... to be modified by alternative splicing--which has ... SpliceArrays you can perform a classical expression ... the different alternatively spliced forms of the ...
... form of cell suicide, which follows a ... of this process is a cascade of ... participate in a series of reactions that ... and result in the cleavage of protein ...
...
Biology Products: