Navigation Links
Is RNA Amplification Necessary for Microarrays?

ether the individual genes that were differentially expressed in amplified samples would have been identified as such using total RNA. For this experiment, the number of "outliers" defined by authors as "A gene with expression ratios of greater than or equal to 2.0 or less than or equal to 0.5 in two reciprocal microarray analysis" identified using total RNA or amplified RNA (one or two rounds of amplification) were compared. The amplified samples identified approximately 80% of the genes selected as outliers with total RNA, with an 80% concordance between target made by one and two rounds of amplification. Thus amplification yielded approximately 2 times more outliers than total RNA, with 80% of these showing similar trends in expression between amplified and total RNA samples. Validation of the "outliers" between total RNA and amplified RNA was carried out using real-time PCR analysis. The PCR data suggested that the majority of outliers identified using amplified RNA are valid. Thus Feldman et al. also conclude that all RNA samples should undergo at least one round of amplification to maximize the quality of array data for detection of differentially expressed genes.

SYBR Green is a registered trademark of Molecular Probes, Inc.
Cy is a trademark of Amersham Biosciences.


back to top


Ordering Information
Cat
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Greater Amplification Specificity with New Hot Start PCR Enzyme
4. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
8. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
9. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
10. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
11. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNA Amplification Necessary for Microarrays

(Date:9/19/2014)... 19, 2014  InterMune, Inc. (Nasdaq: ITMN ... of Understanding to settle the litigation in the Delaware ... of Merger dated as of August 22, 2014, among ... As one term of the Memorandum of Understanding, the ... in the attached exhibit reflecting the amendment to the ...
(Date:9/19/2014)... Sept. 19, 2014  An entire month is dedicated ... neuropathies – Charcot-Marie-Tooth disease (CMT) – a debilitating neuromuscular ... extreme muscle atrophy, weakness, and foot and hand deformities. ... millions worldwide. CMT is a progressive disease, and over ... requiring mobility devices such as leg braces, wheelchairs and ...
(Date:9/19/2014)... (PRWEB) September 19, 2014 Barnhardt ... Product Label for its Organic Cotton . The ... amount of renewable biobased ingredients meets or exceeds levels ... materials composed in whole or in significant part of ... will allow our Organic cotton to be immediately identified ...
(Date:9/18/2014)... BELLINGHAM, Washington, USA (PRWEB) September 18, 2014 ... of Representatives of a bill designed to stimulate development ... high-value jobs is being praised by leaders of ... The bill, the Revitalizing American Manufacturing and Innovation (RAMI) ... vote. The bill’s authors have said they are optimistic ...
Breaking Biology Technology:InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 2InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 3InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 4InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 5InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 6InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 7InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 8InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 9InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 10InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 11InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 12InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 13InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 14InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 15InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 16InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 17InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 18InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 19September is Charcot-Marie-Tooth (CMT) Awareness Month - Scheduled events, activities and important partnerships announced throughout the month! 2Barnhardt Manufacturing Company Earns USDA Certified Biobased Product Certification and Label for Organic Cotton 2House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 2House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 3
... Debiopharm Group,(Debiopharm), a global independent biopharmaceutical development ... oncology, today,announced the establishment of a Debiopharm ... Lausanne (EPFL), focusing on Signal Transduction,in Oncogenesis. ... the objective,of the chair is to leverage ...
... to generate antibodies against ... research targets provided by Sigma-Aldrich, MUNICH, Germany ... SIAL ) and MorphoSys AG (Frankfurt Stock,Exchange: MOR; ... to design, produce and distribute unique,recombinant research antibodies ...
... of,Brazil,s leading distributors of pharmaceutical products, Profarma,Distribuidora de Produtos Farmaceuticos ... results for the fourth quarter of,2007 (4Q07) and full year ... market share breaks record and reaches 12.8% which is 0.8 ... in the 3Q07 and 3.2 percentage ...
Cached Biology Technology:Debiopharm and EPFL Establish an Oncology Chair 2Sigma-Aldrich and MorphoSys Enter Into a Collaboration and Licensing Agreement for Recombinant Research Antibodies 2Sigma-Aldrich and MorphoSys Enter Into a Collaboration and Licensing Agreement for Recombinant Research Antibodies 3
(Date:9/19/2014)... Oklahoma biology professor will study the unique bioelectric signaling ... eventually benefit people,s health. The OU researcher is ... required to produce the signals used to map the ... at a rate of 500-600 discharges a second throughout ... electric fish are extreme, but necessary for survival. , ...
(Date:9/19/2014)... , Sept. 19, 2014 Nxt-ID, Inc. (Nasdaq: ... authentication company focused on the growing m-commerce market, updates the "Wocket ... champion in the lightweight and light middleweight weight classes, Vinny ... it was announced that Game of Thrones actor ... Married with Children star Katey Sagal have been ...
(Date:9/18/2014)... located deep in the primitive brainstem has revealed how ... Harvard School of Medicine and the University at Buffalo ... the second "sleep node" identified in the mammalian brain ... to produce deep sleep. , Published online in August ... fully half of all of the brain,s sleep-promoting activity ...
Breaking Biology News(10 mins):NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 2NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 3No sedative necessary: Scientists discover new 'sleep node' in the brain 2
... design for new cancer therapies has historically been focused on the ... at Memorial Sloan-Kettering Cancer Center published on September 26 in ... genomic signature of a tumor rather than the tissue of origin ... of cancer is a multi-step process, but because of the efforts ...
... revealed that coastal animals have their own biological tidal ... Experts from the University,s Department of Genetics have ... reveals the discovery of an independent clock driving coastal ... Circadian and Circatidal Timekeeping in the Marine Crustacean ...
... all of which are used to help make decisions. Sound ... But in a new book Hear Where We Are: ... Executive Director of Ocean Conservation Research, proposes that sound serves ... means of helping people to form relationships with their environment. ...
Cached Biology News:Oncogenic signatures mapped in TCGA a guide for the development of personalized therapy 2Coastal animals have their own tidal timer -- separate from the 24-hour body clock 2Hear where you are: How sound not only informs, but places us 2
...
More cell-permeant than acridine orange....
Anti-rat Ig HRP detection kit...
One bottle containing 30 ml of protein solution and 0.1% sodium azide....
Biology Products: