Navigation Links
Is RNA Amplification Necessary for Microarrays?

ether the individual genes that were differentially expressed in amplified samples would have been identified as such using total RNA. For this experiment, the number of "outliers" defined by authors as "A gene with expression ratios of greater than or equal to 2.0 or less than or equal to 0.5 in two reciprocal microarray analysis" identified using total RNA or amplified RNA (one or two rounds of amplification) were compared. The amplified samples identified approximately 80% of the genes selected as outliers with total RNA, with an 80% concordance between target made by one and two rounds of amplification. Thus amplification yielded approximately 2 times more outliers than total RNA, with 80% of these showing similar trends in expression between amplified and total RNA samples. Validation of the "outliers" between total RNA and amplified RNA was carried out using real-time PCR analysis. The PCR data suggested that the majority of outliers identified using amplified RNA are valid. Thus Feldman et al. also conclude that all RNA samples should undergo at least one round of amplification to maximize the quality of array data for detection of differentially expressed genes.

SYBR Green is a registered trademark of Molecular Probes, Inc.
Cy is a trademark of Amersham Biosciences.


back to top


Ordering Information
Cat
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
3. Greater Amplification Specificity with New Hot Start PCR Enzyme
4. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
5. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
8. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
9. Optimizing the Direct Amplification of Missing cDNA 5 Ends Using the Eppendorf Mastercycler gradient
10. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
11. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: RNA Amplification Necessary for Microarrays

(Date:1/15/2014)... 15, 2014  Bill Jacobs Automotive, a group of car dealerships ... with the Heartland Blood Center and offering free oil change ... takes place Saturday, Jan. 18 at the Bill Jacobs Cadillac ... (Photo: http://photos.prnewswire.com/prnh/20140115/MN46637 )  The Bill ...
(Date:1/15/2014)... England and SAN JOSE, California ... international biotechnology company developing antibody-drug conjugates for cancer, today announced ... Board of Directors. Dr Reynolds has over 20 years, development ... Medical Officer at Seattle Genetics. "I am delighted ...
(Date:1/15/2014)... January 15, 2014 Two champions of ... their sponsorship of an annual competition for middle and ... access to, innovative STEM study. The competition presents students ... Tests of Engineering Aptitude, Mathematics, and Sciences is ...
(Date:1/15/2014)... 15, 2014 AudioNotch is the internet's ... for the treatment of tinnitus. Patients listen to sound ... over a period of weeks to months, their tinnitus volume ... two forms: Notched Music and Notched White Noise. Now, AudioNotch ...
Breaking Biology Technology:Join the Bill Jacobs Auto blood drive and receive a free oil change 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 4Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 5
... MCLEAN, Va., Aug. 3, 2011 HumanTouch, LLC today ... 2011 Fast 50 Government Contractors list.   ... to adapt to ever-changing market conditions and build a ... companies that populate the Fast 50 range from resellers ...
... Omeros Corporation (NASDAQ: OMER ), a biopharmaceutical ... on inflammation, bleeding and disorders of the central nervous ... and chief executive officer, is scheduled to present at ... Genuity Growth Conference August 10, 2011 9:00 a.m. ...
... 2011 Cambrex Corporation (NYSE: CBM ) reports second ... Highlights Reported sales increased by ... compared to the second quarter of 2010. EBITDA was $13.2 ... compared to $12.1 million in the second quarter of 2010. ...
Cached Biology Technology:Cambrex Reports Second Quarter of 2011 Results 2Cambrex Reports Second Quarter of 2011 Results 3Cambrex Reports Second Quarter of 2011 Results 4Cambrex Reports Second Quarter of 2011 Results 5Cambrex Reports Second Quarter of 2011 Results 6Cambrex Reports Second Quarter of 2011 Results 7Cambrex Reports Second Quarter of 2011 Results 8
(Date:4/17/2014)... ALBUQUERQUE, N.M. A credit-card-sized anthrax detection cartridge developed ... small business makes testing safer, easier, faster and cheaper. ... causes anthrax, is commonly found in soils all over ... illness in both humans and animals. The bacteria can ... to B. anthracis may occur through skin ...
(Date:4/17/2014)... Kansas State University engineer has developed a patented ... improvised explosive devices. The same technique could help ... Steven M. and Kay L. Theede chair in ... engineering, and his research team have created a ... in car trunks. The distance detection method ...
(Date:4/17/2014)... professor Rajendra Singh Thursday as a "Champion of ... solar deployment in the residential, commercial and industrial ... of Electrical and Computer Engineering and director of ... local hero leading the charge across the country ... power and driving policy changes at the local ...
Breaking Biology News(10 mins):Pocket-sized anthrax detector aids global agriculture 2Pocket-sized anthrax detector aids global agriculture 3Patented research remotely detects nitrogen-rich explosives 2White House honors Clemson professor as 'Champion of Change' for solar deployment 2
... working with Professor Dr. Dr. Perikles Simon, head of the ... Germany, suggest in the light of recent analyses that German ... up to the age of five years, today,s children,s weight ... Then as now there are about 10 percent of the ...
... sitting on a goal post could attract a male moth on ... switched her scent over time, the male could still find her ... A team of researchers led by Montana State University entomologist ... even the slightest change in the chemicals female moths emit to ...
... of chemical attacks and help save lives, it,s vital ... deadly chemicalssuch as the nerve gas sarin and other ... a new chemical sensor that can simultaneously identify a ... paper describing the system has been published today in ...
Cached Biology News:When do German children gain weight? 2When do German children gain weight? 3Team discovers reason that male moths can keep finding females 2Team discovers reason that male moths can keep finding females 3Hearing the telltale sounds of dangerous chemicals 2Hearing the telltale sounds of dangerous chemicals 3