An Innovative Technology for the Rapid Purification of Endotoxin-Free Plasmid DNA
Dave Cutter and Goodarz Goodarzi
The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit offers a novel and efficient method of isolating endotoxin-free plasmid DNA. A patent-pending endotoxin removal process has resulted in the fastest, most streamlined protocol available for purifying high-quality, endotoxin-free DNA (≤ 0.1 EU/g). This product delivers significant time-savings, overall higher yields, and better transfection efficiencies compared to anion-exchange methods.
Endotoxins are a common contaminant in nucleic acid preparations, particularly when purifying plasmid DNA. The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit is a novel system that has been developed for the rapid purification of endotoxin-free plasmid DNA from a bacterial culture. Comparisons among the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit, the GenElute HP Maxiprep Kit and other plasmid purification systems were performed. The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit sets a new standard for endotoxin-free plasmid purification while saving time and delivering higher yields.
In typical plasmid DNA purification systems, high concentrations of endotoxins are released into the lysate. Like DNA, endotoxins are negatively charged and co-purify with plasmid DNA in both silica-based and anion-exchange-based systems. Endotoxins can also form large aggregates resulting in co-purification in size exclusion columns and cesium chloride density gradients. Therefore, both standard and traditional plasmid purification systems can result in high levels of endotoxin contaminating the purified plasmid DNA.
While not every molecular biology technique requires the use of endotoxin-free plasmid DNA,
transfection of endotoxin-sensitive cell lines is a major appli