Introducing,,,GenElute HP,,,Endotoxin-Free Plasmid,,,Maxiprep Kit
An Innovative Technology for the Rapid Purification of Endotoxin-Free Plasmid DNA
Dave Cutter and Goodarz Goodarzi
The GenElute HP Endotoxin-Free Plasmid Maxiprep
Kit offers a novel and efficient method of isolating
endotoxin-free plasmid DNA. A patent-pending
endotoxin removal process has resulted in the fastest,
most streamlined protocol available for purifying
high-quality, endotoxin-free DNA (≤ 0.1 EU/g).
This product delivers significant time-savings, overall
higher yields, and better transfection efficiencies
compared to anion-exchange methods.
Endotoxins are a common contaminant in nucleic acid preparations, particularly when purifying plasmid DNA. The GenElute
HP Endotoxin-Free Plasmid Maxiprep Kit is a novel system that has been developed for the rapid purification of endotoxin-free
plasmid DNA from a bacterial culture. Comparisons among the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit, the
GenElute HP Maxiprep Kit and other plasmid purification systems were performed. The GenElute HP Endotoxin-Free Plasmid
Maxiprep Kit sets a new standard for endotoxin-free plasmid purification while saving time and delivering higher yields.
In typical plasmid DNA purification systems, high concentrations of endotoxins are released into
the lysate. Like DNA, endotoxins are negatively charged and co-purify with plasmid DNA in both
silica-based and anion-exchange-based systems. Endotoxins can also form large aggregates
resulting in co-purification in size exclusion columns and cesium chloride density gradients.
Therefore, both standard and traditional plasmid purification systems can result in high levels of
endotoxin contaminating the purified plasmid DNA.
While not every molecular biology technique requires the use of endotoxin-free plasmid DNA,
transfection of endotoxin-sensitive cell lines is a major appliPage: All 1 2 3 4 5 Related biology technology :1
. Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)2
. Choose the Right Transfection Agent for Your RNAi Experiment
(Date:1/15/2014)... (PRWEB) January 15, 2014 Two ... education have continued their sponsorship of an annual ... to stimulate interest in, and access to, innovative ... problems experienced by engineers. , TEAMS: Tests ... a program administered by the Technology Student Association ...
(Date:1/15/2014)... Freeslate, Inc ., the ... announced that Lupin Limited, one of India’s top ... CM Protégé PharmD System for high ... in Mumbai, India, is focused on a wide ... and Active Pharmaceutical Ingredients (APIs) for the developed ...
(Date:1/14/2014)... Carahsoft and CDS Federal Services ... 23, 2014 at 2pm EST (11am PST), “Natural ... The topic focuses on how technology can turn ... mission critical decisions for government agencies. The online ... Big Data has become an obstacle for government ...
(Date:1/14/2014)... York, NY (PRWEB) January 14, 2014 ... initiated coverage of Alliqua, Inc. (OTCQB: ALQA). Alliqua ... and marketing proprietary products to serve the wound ... , In late 2012, Alliqua was restructured ... launched the company’s new strategy to become a ... Breaking Biology Technology:Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 4Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 5Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3
... Emerging Nanotechnologies (PEN) and National Science Foundation (NSF) ... the television series Nanotechnology: The Power of Small ... onlywill include remarks by U.S. Senator Ron Wyden ... , The series three programs explore critical questions ...
... Beckman Coulter,Inc. (NYSE: BEC ), a leading ... and innovate complex biomedical testing,announced today that Paul ... Business Development, will present at the 2008 Bear ... at 4:00 a.m.,(ET)., (Logo: http://www.newscom.com/cgi-bin/prnh/20031202/BECLOGO ), ...
... Inc.,(Nasdaq: ONTY ) (TSX: ONY) (the "Company") today ... 31, 2007. All results are in U.S.,dollars and prepared ... ended December 31, 2007, was $20.3 million or,$1.04 per ... $2.38,per basic and diluted share in 2006. The substantial ...Cached Biology Technology:New nanotechnology television series does 'sweat the small stuff' 2Beckman Coulter to Present at the 2008 Bear Stearns London Healthcare Conference 2Oncothyreon reports full year and fourth quarter 2007 financial results 2Oncothyreon reports full year and fourth quarter 2007 financial results 3Oncothyreon reports full year and fourth quarter 2007 financial results 4Oncothyreon reports full year and fourth quarter 2007 financial results 5Oncothyreon reports full year and fourth quarter 2007 financial results 6Oncothyreon reports full year and fourth quarter 2007 financial results 7
(Date:3/10/2014)... found that oral glucosamine supplementation is not associated with ... chronic knee pain. Findings published in Arthritis & ... Rheumatology (ACR) journal, indicate that glucosamine does not decrease ... as bone bruises and thought to be a source ... According to the ACR 27 million Americans over 25 ...
(Date:3/10/2014)... Mo. As energy costs rise, more Americans are ... and workplaces. Bioenergy is renewable energy made from organic ... biomass power plants small enough to fit on a ... University of Missouri researchers have found that creating a ... in rural areas of the country as well as ...
(Date:3/10/2014)... the mildest form of a traumatic brain injury, better ... Now, an interdisciplinary team of researchers at the University ... the mechanisms at play in this kind of injury, ... long-term consequences. , Their recent findings, published in the ... mechanical properties of a critical brain protein and its ... Breaking Biology News(10 mins):Glucosamine fails to prevent deterioration of knee cartilage, decrease pain 2Small biomass power plants could help rural economies, stabilize national power grid, MU study finds 2Penn researchers model a key breaking point involved in traumatic brain injury 2Penn researchers model a key breaking point involved in traumatic brain injury 3Penn researchers model a key breaking point involved in traumatic brain injury 4
... ways in which fruit flies might react to extreme ... ("heat hardening") has been known to have negative effects ... can have advantages too. Flies exposed to heat hardening ... bait on very hot days than were the flies ...
... 'tree of life' tracing the history of all 4,500 mammals ... result of the death of the dinosaurs, says new research ... the UK by scientists at Imperial College London and the ... accepted theory that the Mass Extinction Event (MEE) that wiped ...
... (SLE), often simply called lupus, is a complex ... extreme fatigue, and depression, among other symptoms. Some ... most severe psychiatric manifestation, psychosis, and a protein ... P. , To investigate how an ...Cached Biology News:Dinosaur extinction didn't cause the rise of present-day mammals, claim researchers 2New insights into autoimmunity and depression 2
... is a full featured, two laser, six detector ... Cytometer, CFlow Software, and a PC for operation ... all the capabilities of the best selling flow ... C6 Flow Cytometer System has six detectors to ...
... capability allows detection of SYBR Green I ... a range of fluorophores in the second ... TAMRA for a multitude of applications ... Engine thermal cycler offers precision thermal control ...
... Fingerprinting II identification ... an optional module that ... similarity searches and identification ... library of entries of ...
Fingerprinting II comparative quantification and polymorphism analysis is an optional module that adds comparative quantification and polymorphism analysis functions to the Fingerprinting II Basic so...Biology Products: