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Intracytoplasmic Spermatocyte Injection (ICSI)

The number of infertile couples has increased in recent years and is now estimated at 1520% of all couples worldwide. Since its introduction into reproductive medicine in 1978 [1] , in vitro fertilization has been considered a successful method of treating tubal sterility. With increasingly intensive diagnosis of both partners, certain aspects of male subfertility are coming to the forefront. Nowadays almost 50% of insterility in couples can be attributed to male subfertility [2] . Intracytoplasmatic sperm injection (ICSI) has raised hopes that these couples can have children of their own. In the meantime this method of treating male subfertility has achieved a breakthrough [3] and is gaining in importance. It is establishing itself as the preferred method of treatment in the field of assisted reproduction, following numerous pregnancies and births [4] . Preparation for ICSI involves obtaining an oocyte by means of follicle aspiration, removal of the cumulus cells and of the corona radiata and preparation of semen in accordance with the Mini Swim-Up process [5] or other preparation procedures [6]. Thereafter, one sperm is injected into this oocyte with a thin glass capillary (pipette). If fertilization has occurred, the embryo is transferred into the uterus 48 hours after microinjection. For the microinjection process described, Eppendorf offers ideal tools for easing the workload: the micromanipulator TransferMan, used for moving the holding capillary, and a second micro-manipulator TransferMan for transferring the sperm.
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