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Integration of the LEADseeker Multimodality Imaging System with automated plate loading and incubation using Overlord Instrument software

f the process of robot position teaching and procedure development are beyond the scope of this application note. Further information can be obtained from the instrument suppliers (Caliper Technologies Inc) or from Process and Automation Analysis Ltd.

Kendro Heraeus Cytomat 6001 Incubator settings
A Cytomat 6001 Incubator was set to run at 30 °C. No changes were made to any other settings. The overall size of the Twister II grippers and the robot head is such that it cannot access the plate transfer station of the incubator. To facilitate entry of the robot to this position, the casing over the plate entry door was modified (raised) to give the necessary clearance. Similar modifications should be confirmed first with the instrument supplier.

LEADseeker instrument settings
The LEADseeker instrument was used in radiometric imaging mode to test reproducibility of a range of 384well [14C] Uniform 384-Well Reference Plates. For convenience, the exposure time on the system was set to 10 s. During acquisition, the LEADseeker computer displays the communication dialogue between the LEADseeker instrument and the Overlord computer in the Connect window (Fig 4).


Results
Typical data from reading a group of [14C]Uniform 384-Well Reference Plates (23 separate plates) is shown in Figure 5. The data shows that each plate was successfully loaded from the incubator into the LEADseeker instrument via the Zymark Twister II Robot and subsequently read using the protocol requested via the Overlord Instrument software. The exported data was then used to plot the mean signal ±3 SD (n = 384) from each 384-well plate.


Conclusion
The LEADseeker Multimodality Imaging System can be successfully integrated
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