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Integration of the LEADseeker Multimodality Imaging System with automated plate loading and incubation using Overlord Instrument software

Key words: LEADseeker • imaging • automation • integration


The LEADseeker™ Multimodality Imaging System is a CCD-based optical imaging instrument that efficiently detects and quantitates light from a range of radiometric, luminescence, and fluorescence assays. Throughput of over 100 plates per hour is possible due to the combination of format-free imaging (fluorescence assays can be read in as little as 10 s) and the instrument’s precise and rapid plate delivery mechanism. The instrument can be integrated with most automation devices via its open architecture software.

Here, we describe the integration of a LEADseeker instrument with a Kendro Heraeus™ Cytomat™ Incubator and a Zymark™ Twister™ II Robot, using Overlord™ Instrument software to control all the instruments.


Materials
Products used
LEADseeker Multimodality Imaging System 18-1140-71

[14C]Uniform 384-Well Reference Plate, CFQ11387
0.148 MBq, 4 µCi


Other material required
Overlord Instrument software (Process Analysis and Automation Ltd.)

Zymark Twister II Robot (Caliper Life Sciences Ltd.)

Cytomat 6001 Incubator (Kendro Laboratory Products PLC.)


Method
Overlord communication settings
Overlord Instrument software was installed on a PC and used to control a Zymark Twister II Robot, Cytomat 6001 Incubator, and the LEADseeker instrument. Connections to the robot and the incubator were made by conventional 9pin D connector RS-232 serial cables. The connection between the Overlord PC and the LEADseeker instrument PC was by a null modem cabl
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