Navigation Links
Integration of the LEADseeker Multimodality Imaging System with automated plate loading and incubation using Overlord Instrument software

Key words: LEADseeker • imaging • automation • integration


The LEADseeker™ Multimodality Imaging System is a CCD-based optical imaging instrument that efficiently detects and quantitates light from a range of radiometric, luminescence, and fluorescence assays. Throughput of over 100 plates per hour is possible due to the combination of format-free imaging (fluorescence assays can be read in as little as 10 s) and the instrument’s precise and rapid plate delivery mechanism. The instrument can be integrated with most automation devices via its open architecture software.

Here, we describe the integration of a LEADseeker instrument with a Kendro Heraeus™ Cytomat™ Incubator and a Zymark™ Twister™ II Robot, using Overlord™ Instrument software to control all the instruments.


Materials
Products used
LEADseeker Multimodality Imaging System 18-1140-71

[14C]Uniform 384-Well Reference Plate, CFQ11387
0.148 MBq, 4 µCi


Other material required
Overlord Instrument software (Process Analysis and Automation Ltd.)

Zymark Twister II Robot (Caliper Life Sciences Ltd.)

Cytomat 6001 Incubator (Kendro Laboratory Products PLC.)


Method
Overlord communication settings
Overlord Instrument software was installed on a PC and used to control a Zymark Twister II Robot, Cytomat 6001 Incubator, and the LEADseeker instrument. Connections to the robot and the incubator were made by conventional 9pin D connector RS-232 serial cables. The connection between the Overlord PC and the LEADseeker instrument PC was by a null modem cable* (Fig 1).

The LEADseeker instrument PC is supplied with a DIGI multiport serial card to allow connection of up to eight serial devices, although the LEADseeker instrument is controlled through COM1 and COM2 so these ports cannot be used for other equipment. The cables from the DIGI card are labeled P1 to P8 and their COM port equivalents are shown in Figure 1 and Figure 2.

Overlord program
Overlord Instrument software has a simple drag and drop interface for setting up communication between instruments. The software also permits a range of programming elements such as numeric and string variables, conditional statements (e.g., IF THEN, ELSE), and control loops (e.g., FORNEXT). A simple program typically used in this work is summarized in Figure 3.

The purpose of the Overlord program illustrated in Figure 3 was to take a number of microplates from storage in the Cytomat Incubator, use the Zymark Twister II Robot to transfer them to the LEADseeker instrument for data acquisition, and then return the microplates to the Cytomat Incubator for storage or further incubation. The program allows the user to enter the number of plates to be processed and the number of the plate with which the run starts.

Following data acquisition, the numeric file associated with the processed LEADseeker image was stored on the Overlord computer by transfer across the serial connection. The filename assigned for each file was generated by string concatenation shown in Figure 3.

Zymark Twister II Robot settings
The Zymark Twister II Robot was taught its positions and procedures using Overlord Instrument software. Robot procedures were given self-explanatory names such as GetPlateFromCytomat, PutPlateOnLEADseeker etc. Full descriptions of the process of robot position teaching and procedure development are beyond the scope of this application note. Further information can be obtained from the instrument suppliers (Caliper Technologies Inc) or from Process and Automation Analysis Ltd.

Kendro Heraeus Cytomat 6001 Incubator settings
A Cytomat 6001 Incubator was set to run at 30 °C. No changes were made to any other settings. The overall size of the Twister II grippers and the robot head is such that it cannot access the plate transfer station of the incubator. To facilitate entry of the robot to this position, the casing over the plate entry door was modified (raised) to give the necessary clearance. Similar modifications should be confirmed first with the instrument supplier.

LEADseeker instrument settings
The LEADseeker instrument was used in radiometric imaging mode to test reproducibility of a range of 384well [14C] Uniform 384-Well Reference Plates. For convenience, the exposure time on the system was set to 10 s. During acquisition, the LEADseeker computer displays the communication dialogue between the LEADseeker instrument and the Overlord computer in the Connect window (Fig 4).


Results
Typical data from reading a group of [14C]Uniform 384-Well Reference Plates (23 separate plates) is shown in Figure 5. The data shows that each plate was successfully loaded from the incubator into the LEADseeker instrument via the Zymark Twister II Robot and subsequently read using the protocol requested via the Overlord Instrument software. The exported data was then used to plot the mean signal ±3 SD (n = 384) from each 384-well plate.


Conclusion
The LEADseeker Multimodality Imaging System can be successfully integrated with the Zymark Twister II Robot and the Kendro Heraeus Cytomat Incubator and controlled using Overlord Instrument software.


* In situations where the LEADseeker instrument PC is required to drive the robot and any other equipment, a null modem cable is required to connect COM ports 5 and 6 together on the LEADseeker instrument PC. This is because the LEADseeker instrument can only accept instructions from an outside source via a serial cable, even though Overlord Instrument software is resident on the same PC.

† The LEADseeker instrument usually expects to receive input signals through COM5; this is configured in the MS Windows registry and can be confirmed using REGEDIT.EXE.



back to top
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
2. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
3. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
4. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
5. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
6. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
7. Performance of fluorescent dyes with LEADseeker Multimodality Imaging System
8. Use of the HitHunter EFC Chemiluminescent Detection Kits with LEADseeker Multimodality Imaging System
9. Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System
10. Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System
11. The development of a somatostatin sst4 receptor-binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
(Date:7/29/2015)... July 30, 2015 According to a ... Growth, Trends & Forecasts (2015-2020), , published by Mordor Intelligence, ... billion by the end of 2020, with North ... more than 40% of the global market size. The Global ... CAGR of 13.7% during the period of (2015-2020). ...
(Date:7/29/2015)... 29, 2015  HealthSouth Corporation (NYSE: ... of post-acute healthcare services, offering both facility-based and ... operations for the second quarter ended June 30, 2015. ... by strong volume growth in both segments and ... Jay Grinney, President and Chief Executive Officer of ...
(Date:7/29/2015)... , ... July 29, 2015 , ... ... today announced its GHS Label Guide . To align chemical container labeling ... this guide includes explanations of label components, an example of an accurate label, ...
(Date:7/29/2015)...  Indivior PLC (LON: INDV) today announced that the ... accepted and received Priority Review by the U.S. Food ... overdose. This naloxone nasal spray comes as a pre-filled ... into the nasal mucosa. 1 The device has ... be better equipped to help an opioid overdose victim. ...
Breaking Biology Technology:Asia Pacific to Dominate the Global Stem Cell Market - Vericel Corporation, Ocata Therpeutics, Athersys Inc., Cryo-Cell International, Geron Corporation, StemCells, Inc. Among the Major Players 2Asia Pacific to Dominate the Global Stem Cell Market - Vericel Corporation, Ocata Therpeutics, Athersys Inc., Cryo-Cell International, Geron Corporation, StemCells, Inc. Among the Major Players 3HealthSouth Reports Results for Second Quarter 2015 2HealthSouth Reports Results for Second Quarter 2015 3HealthSouth Reports Results for Second Quarter 2015 4HealthSouth Reports Results for Second Quarter 2015 5HealthSouth Reports Results for Second Quarter 2015 6HealthSouth Reports Results for Second Quarter 2015 7HealthSouth Reports Results for Second Quarter 2015 8HealthSouth Reports Results for Second Quarter 2015 9HealthSouth Reports Results for Second Quarter 2015 10HealthSouth Reports Results for Second Quarter 2015 11HealthSouth Reports Results for Second Quarter 2015 12HealthSouth Reports Results for Second Quarter 2015 13HealthSouth Reports Results for Second Quarter 2015 14HealthSouth Reports Results for Second Quarter 2015 15HealthSouth Reports Results for Second Quarter 2015 16HealthSouth Reports Results for Second Quarter 2015 17HealthSouth Reports Results for Second Quarter 2015 18HealthSouth Reports Results for Second Quarter 2015 19HealthSouth Reports Results for Second Quarter 2015 20HealthSouth Reports Results for Second Quarter 2015 21HealthSouth Reports Results for Second Quarter 2015 22HealthSouth Reports Results for Second Quarter 2015 23HealthSouth Reports Results for Second Quarter 2015 24HealthSouth Reports Results for Second Quarter 2015 25HealthSouth Reports Results for Second Quarter 2015 26HealthSouth Reports Results for Second Quarter 2015 27HealthSouth Reports Results for Second Quarter 2015 28HealthSouth Reports Results for Second Quarter 2015 29HealthSouth Reports Results for Second Quarter 2015 30HealthSouth Reports Results for Second Quarter 2015 31HealthSouth Reports Results for Second Quarter 2015 32HealthSouth Reports Results for Second Quarter 2015 33HealthSouth Reports Results for Second Quarter 2015 34
... , ... Feb. 9 Reportlinker.com announces that a new ... The Clinical Laboratory Services Market (Growth Opportunities, ... http://www.reportlinker.com/p0176223/The-Clinical-Laboratory-Services-Market-Growth-Opportunities-Competitive-Analysis-and-Competitor-Profiles.html , Clinical laboratories are an essential part ...
... , SAN ... www.endpointclinical.com ), a leader in Integrated Voice Response (IVR)/Integrated Web ... platform that allows users to design and deploy clinical trial ... development time of clinical trials. This time and cost savings ...
... , , , , , ... 9 /PRNewswire-Asia-FirstCall/ -- Tianyin,Pharmaceutical Co., Inc., (NYSE Amex: ... ("TCM") and Western Pharmaceuticals,based in Chengdu, China , today ... to Reform of Pricing Mechanism of Medicine,and Medical Services by ...
Cached Biology Technology:Reportlinker Adds the Clinical Laboratory Services Market (Growth Opportunities, Competitive Analysis and Competitor Profiles) 2Reportlinker Adds the Clinical Laboratory Services Market (Growth Opportunities, Competitive Analysis and Competitor Profiles) 3Reportlinker Adds the Clinical Laboratory Services Market (Growth Opportunities, Competitive Analysis and Competitor Profiles) 4Reportlinker Adds the Clinical Laboratory Services Market (Growth Opportunities, Competitive Analysis and Competitor Profiles) 5Reportlinker Adds the Clinical Laboratory Services Market (Growth Opportunities, Competitive Analysis and Competitor Profiles) 6Reportlinker Adds the Clinical Laboratory Services Market (Growth Opportunities, Competitive Analysis and Competitor Profiles) 7Reportlinker Adds the Clinical Laboratory Services Market (Growth Opportunities, Competitive Analysis and Competitor Profiles) 8Reportlinker Adds the Clinical Laboratory Services Market (Growth Opportunities, Competitive Analysis and Competitor Profiles) 9Reportlinker Adds the Clinical Laboratory Services Market (Growth Opportunities, Competitive Analysis and Competitor Profiles) 10endpoint Saves Money and Time for Medical Research Teams Through PULSE 2endpoint Saves Money and Time for Medical Research Teams Through PULSE 3Tianyin Pharmaceutical Co., Inc. Expects to Benefit from the Recently Published Guide to Pricing Reform for Medicine and Medical Services 2Tianyin Pharmaceutical Co., Inc. Expects to Benefit from the Recently Published Guide to Pricing Reform for Medicine and Medical Services 3
(Date:7/9/2015)... 9, 2015  Synaptics Inc. (NASDAQ: ... interface solutions, today announced a new Match-in-Sensor™ ... hardware encapsulated fingerprint sensor and matching solution. ... literally off the grid, isolating fingerprint image ... the fingerprint sensor to provide ultimate protection ...
(Date:7/7/2015)... 2015 Research and ... of the "Capacitive Fingerprint Sensors Patent Landscape" ... this date, fingerprint sensing technology is the most ... are well developed. This patent landscape focuses on ... The domain of capacitive fingerprint sensors is closely ...
(Date:7/2/2015)... Fingerprint Cards has received an order for its ... distributor World Peace Industrial Group (WPI), part of WPG Holdings. ... . Deliveries are planned to take place during Q3 ... manufacturers in Asia . The order value ... of approximately 2,200 MSEK for 2015. Jörgen Lantto, ...
Breaking Biology News(10 mins):Synaptics Announces World's First Match-in-Sensor Fingerprint Authentication Technology 2Synaptics Announces World's First Match-in-Sensor Fingerprint Authentication Technology 3Worldwide Capacitive Fingerprint Sensors Patent Landscape Market Report 2015-2020 - Main IP Holders include Seiko Epson, AuthenTec, Sony and STMicroelectronics 2FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2
... SANTA CRUZ, CA--Observations at submarine springs found along the ... preview of the possible fate of coral reef ecosystems ... pH (a measure of acidity) in the water around ... result from the widespread acidification of surface waters that ...
... Electronic infrastructures open new horizons for collaboration and acceleration ... such as scratchpads.eu , yield opportunities, ... newly collected data coming from different sources and working ... journal ZooKeys presenting the results ...
... FAIRFAX, Va.Researchers have published the largest single patient ... following patients with retrievable inferior vena cava (IVC) ... to the lungs. This study, which is important ... supports existing guidelines developed by the Society of ...
Cached Biology News:Submarine springs offer preview of ocean acidification effects on coral reefs 2Submarine springs offer preview of ocean acidification effects on coral reefs 3Electronic infrastructures accelerate biodiversity discoveries 2IVC filters: Society of Interventional Radiology leads in patient care, safety, research 2IVC filters: Society of Interventional Radiology leads in patient care, safety, research 3
Immunogen: Recombinant full length protein, corresponding to amino acids 1-435 of Human AP2M1. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Anti-Dynamin Monoclonal Antibody Description: 100 g Research Focus: neuroscience Storage: -20C Shipping Temperature: 4C...
cysteine and glycine-rich protein 2,...
BKBeta2 potassium channel Antigen: Fusion protein amino acids 1-41 (N-terminus) and 218-235 (C-terminus) of mouse BKBeta2 Accession Number: Q9CZM9...
Biology Products: