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InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C

>2. Resuspend cells in 1 ml of 1x PBS and centrifuge for 1 min at 10,00012,000 rpm.

3. Resuspend cells in autoclaved water at 2030 cells/l.

4. Add 20 l of this cell suspension to 200 l of InstaGene matrix. Incubate at 56C for 1530 min.

Note: InstaGene matrix should be mixed at moderate speed on a magnetic stirrer to maintain the matrix in suspension. The pipet tip used should have a large bore, such as on a 1,000 l pipet tip.

5. Follow the procedure for DNA preparation from whole blood, steps 68.

DNA Preparation From Bacteria
The protocol described below is for the preparation of genomic DNA or episomal DNA from bacteria.
1. Pick an isolated bacterial colony and resuspend it in 1 ml of autoclaved water in a microfuge tube.

2. Centrifuge for 1 min at 10,00012,000 rpm. Remove the supernatant.

3. Follow the DNA preparation procedure for whole blood, steps 58.


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