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Insect Cell Culture Using a BioFlo 110 Benchtop Bioreactor

a high demand for oxygen when producing protein. Maximum growth rates and high cell densities are achieved by keeping the DO at a given setpoint.

Of course, factors such as substrate concentration and metabolite build-up can also be limiting, but these are often more controllable than the inherent physical limitations of the fermentor.

The BioFlo 110 system allowed for the growth of insect cells to high density: 26.4 x 106 cell/ml (91 g/L DCW).

Inclusion of a BioFlo 110 Gas Mix Controller, standard with our Advanced Cell Culture Kit, has the ability to mix the four gases according to culture needs and further enhances the final cell-density.


Conclusion
With the above in mind, we view the high final cell density of 26.4 x 106 cell/ml as proof of the very good fundamental capabilities of the BioFlo 110 system.

Temperature control was excellent using un-chilled (12.8C) tap water as the coolant.

DO and pH control was excellent and remained constant at setpoint.

Overall, with our protocol, the BioFlo 110 system performed extremely well. The BioFlo 110 Advanced Cell Culture Kit is a highly suitable instrument for growing insect cell cultures.



Appendix

More About Spin Filters

What is a Spin Filter?
A spin filter is a simple device that allows you to refresh media while retaining cells in the vessel.

Why should you use a Spin Filter?
When you are running a perfusion process, the object is to cultivate the highest yield possible. The spin filter is an efficient means to retain cells in the vessel, encouraging continued growth while media is exchanged.

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